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The map label for this gene is radB

Identifier: 148642671

GI number: 148642671

Start: 581649

End: 582353

Strand: Reverse

Name: radB

Synonym: Msm_0611

Alternate gene names: 148642671

Gene position: 582353-581649 (Counterclockwise)

Preceding gene: 148642672

Following gene: 148642670

Centisome position: 31.42

GC content: 33.05

Gene sequence:

>705_bases
ATGAAAGTCTTGGCTAATTTTGAGGATAATCATAAAATCCCAACTAATTCAGGTATTGATAATTTATTGGACGGGGGAGT
GGAAAAAGGAACTGTTACTCAGATATTCGGTCCGCCCGGTTCTGGTAAAAGTAATATCTCTTTGGTATTGGCAGTTAATG
TAGCTAAACAGGGTAAAAAAGTTGTTTATGTTGATACTGAAGGGGGAATTTCAATCAACAGAATTAAACAAATAGCAGGG
GAAGATTTTCCTAAAATAGTTAATAATATTATAGTTTTTGAACCGACTTCATTTTTAGAGCAAAATGAAAATCTTAAAAC
TATTGAGCTCTGGATTAGAAAGCATCATGATGATGTGGATTTATGTGTTTTGGATTCAGCTGTTGCTCTTTATCGTGTTG
ATGATATGAAATCTTCAAGACTTAATAAGGAATTAGGTAAACAGATGGGGATTTTAGCTAAAATAGCTCGTAATTATGAT
GTTGCAGTTGTACTTACCAATCAAATTTACAGTTCTTTTGATGATGATAACAAGGACGTTGTTAAAGCAGTCGGAGGAAC
TATTTTGCAGTACTGGAGTAAAACTATTATTCAGTTAGAGCGAAATGATGAGTTTAATAAAAGAGTAGCTACACTAAAAA
GACATAGAAGTATCGGTGAAGGTAAACAGGCTACTTTTAAAATAGTGGAAAGGGGAATTATTTAA

Upstream 100 bases:

>100_bases
TAAAAATCCATCAACAATTATTGATTTGACTGCTGAAGAACCTATTTTTGTTAAAAGGTAAGCTTAAAAATATATACTTT
GTATAACTAAATAATATATT

Downstream 100 bases:

>100_bases
ACATTAATTTTATAAATAACTTAAAATAAATAATTACTTTGGAATTTTATTAGTATTATGTGATTTTTATGAAAAATAAT
TTAAGCAGTTATGAGGAAGG

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKVLANFEDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKKVVYVDTEGGISINRIKQIAG
EDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVDLCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYD
VAVVLTNQIYSSFDDDNKDVVKAVGGTILQYWSKTIIQLERNDEFNKRVATLKRHRSIGEGKQATFKIVERGII

Sequences:

>Translated_234_residues
MKVLANFEDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKKVVYVDTEGGISINRIKQIAG
EDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVDLCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYD
VAVVLTNQIYSSFDDDNKDVVKAVGGTILQYWSKTIIQLERNDEFNKRVATLKRHRSIGEGKQATFKIVERGII
>Mature_234_residues
MKVLANFEDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKKVVYVDTEGGISINRIKQIAG
EDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVDLCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYD
VAVVLTNQIYSSFDDDNKDVVKAVGGTILQYWSKTIIQLERNDEFNKRVATLKRHRSIGEGKQATFKIVERGII

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily

Homologues:

Organism=Homo sapiens, GI23238219, Length=233, Percent_Identity=29.6137339055794, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI19924133, Length=200, Percent_Identity=29, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI10835029, Length=184, Percent_Identity=31.5217391304348, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI19924117, Length=184, Percent_Identity=31.5217391304348, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI46255039, Length=184, Percent_Identity=31.5217391304348, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI256017143, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI256017141, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71997301, Length=240, Percent_Identity=27.9166666666667, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71997295, Length=240, Percent_Identity=27.9166666666667, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321027, Length=232, Percent_Identity=31.4655172413793, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320942, Length=230, Percent_Identity=30, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24651285, Length=230, Percent_Identity=29.5652173913043, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17864108, Length=230, Percent_Identity=29.5652173913043, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RADB_METS3 (A5UKT8)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001273184.1
- ProteinModelPortal:   A5UKT8
- SMR:   A5UKT8
- STRING:   A5UKT8
- GeneID:   5216190
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0611
- eggNOG:   arNOG05079
- HOGENOM:   HBG413235
- OMA:   ITQIYGP
- ProtClustDB:   PRK09361
- HAMAP:   MF_00350
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588
- InterPro:   IPR004504
- PANTHER:   PTHR22942:SF9
- PIRSF:   PIRSF003336
- PRINTS:   PR01874
- SMART:   SM00382
- TIGRFAMs:   TIGR02237

Pfam domain/function: PF08423 Rad51

EC number: NA

Molecular weight: Translated: 26068; Mature: 26068

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLANFEDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKK
CCEECCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCCE
VVYVDTEGGISINRIKQIAGEDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVD
EEEEECCCCCCHHHHHHHHCCHHHHHHCCEEEECCHHHHHCCCCCCCEEEEEHHCCCCCE
LCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYDVAVVLTNQIYSSFDDDNKDV
EEEEHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCHHH
VKAVGGTILQYWSKTIIQLERNDEFNKRVATLKRHRSIGEGKQATFKIVERGII
HHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLANFEDNHKIPTNSGIDNLLDGGVEKGTVTQIFGPPGSGKSNISLVLAVNVAKQGKK
CCEECCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCCE
VVYVDTEGGISINRIKQIAGEDFPKIVNNIIVFEPTSFLEQNENLKTIELWIRKHHDDVD
EEEEECCCCCCHHHHHHHHCCHHHHHHCCEEEECCHHHHHCCCCCCCEEEEEHHCCCCCE
LCVLDSAVALYRVDDMKSSRLNKELGKQMGILAKIARNYDVAVVLTNQIYSSFDDDNKDV
EEEEHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCHHH
VKAVGGTILQYWSKTIIQLERNDEFNKRVATLKRHRSIGEGKQATFKIVERGII
HHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA