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The map label for this gene is gltB [C]

Identifier: 148642428

GI number: 148642428

Start: 349434

End: 350930

Strand: Reverse

Name: gltB [C]

Synonym: Msm_0368

Alternate gene names: 148642428

Gene position: 350930-349434 (Counterclockwise)

Preceding gene: 148642429

Following gene: 148642426

Centisome position: 18.94

GC content: 38.94

Gene sequence:

>1497_bases
ATGCCATATAGAGTAGAAAGAAATCCGGTTTTATGTAAGAAAAACTTTGGAAGGCCTGGTTGTTGTTGGTATTTGTGTGA
TGACAGGGATGAAAAAATCTGTGGAAGATGTTTCTCATGTTATAATAACTGTCCTCACGGAGTTTATGAAATTATTCAGG
GTGAACCGTACCCATTAAATCAGGAAAAATGTGTCGGGTGCAGAATTTGTTTGGAAATGTGTCCAAATAGGGCTATTGAA
GTAAATGCAATTCCGCAGGATGCAAGAGAAGCATGGGGATTCCCTGATGTTGTGGAAATAGTAAGAAAAGCTCAGTCTGC
ATCATATAAAATCAGAAGTACTGGAGCTTTAAGAAAAATTCCTGACTTTGATGATTTGGTAGTAATACCTGCACAGGTAT
CAAGACCTCCTATTGATAAATATAGGGAACCATGCGGAACAGATGTTGTATTGGGAGATAGATATGCTGAAAATCCTTTA
AAATTAGATACTCCTGTAATGATTGGAGCAATGTCTTTTGGTGCGTTAAGTAAAGAAGCAAAAATGGCATTAGCTATTGG
TTCTTCACTTGCAGGAACTGTAACAAACACTGGGGAAGGGGGAATGCTTCCTGAAGAACGTGAATTGGCTGATAAACTTA
TAGCACAGTATGCATCTGGAAGATTTGGTGTTTCTGCAGATTATCTTAAACAGGGAGATGCAGTTGAAATCAAAATAGGT
CAGGGGGCAAAATCCGGAATGGGTGGTCACTTGCTTGGTGAAAAAGTAACTGCAGAAGTTTCCAGAATCAGAAAAATTCC
TGTAGGGTCTGATGCTCTATCACCTGCAAGACACATGGATATTGTAGGTCCTGAAGATTTAAGTATGAAGATTTCTCAAT
TACGTGAAATTACTGACTGGAAAGTTCCGATCATTGTTAAATTTGCTTCAGGTAAAGTAGCTTCTGATGTAAAAATCGCA
GCTAAGGGTGGAGCAGATATAATTGTTGTAGACGGTATGCAGGGAGGAACTGGAGCGGGACCTGATGTTATTATGGAACA
TTCAGGTATTCCATCACTTGCAGCTATTGTGGAAGCAGATCAGGCATTAAAGGAAATTAATTTAAGAGAAGATGTAAGTT
TGGTAGCTGCTGGAGGAATTCGTTCTGGAGCAGATTTAGCTAAAGCATTGGCTCTTGGAGCAGATGCAGTATATATTGCA
ACAGCTGCATTGATTTCTATAGGCTGCAGGGTCTGTCAAATGTGTTATAAAGGTAACTGTAGTAAAGGAATAGCTACACA
GGATCTGTCCTTAAGACACAGACTTGATTACAAGGAAATGGGTAAAGCAGTAGCTAATTATATAAATGCAATGACTGATG
AGGCATGTATGCTTGTTCAACAGGCAGGAAACACCCATATTACAAAATTGGAAAAACAAAACTTAAGGGCCTTAACAATG
GAAGCATCAGCTTTAACCGGTGTTCCAATGGCAGGTTCTGAAAACAGAAAAAATTAA

Upstream 100 bases:

>100_bases
GATTTTGATTTAAAGGAAGATGAGTACGAAGAATTTAAAAAAATAGTTCCGGAAAGTGCAAGACCATTTTACGGTCATTA
ATTGAATAGGAGGGAAGTGT

Downstream 100 bases:

>100_bases
ATTCTGGGGGTTTGTTCAGCTAGACATAAATTGCAGATAGCATTTTCATTGTCTTTTTGTTTGTCTTTAACTGGACAGTA
AAACTCCCCATTTTTTTCTT

Product: glutamate synthase (NADPH), subunit 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MPYRVERNPVLCKKNFGRPGCCWYLCDDRDEKICGRCFSCYNNCPHGVYEIIQGEPYPLNQEKCVGCRICLEMCPNRAIE
VNAIPQDAREAWGFPDVVEIVRKAQSASYKIRSTGALRKIPDFDDLVVIPAQVSRPPIDKYREPCGTDVVLGDRYAENPL
KLDTPVMIGAMSFGALSKEAKMALAIGSSLAGTVTNTGEGGMLPEERELADKLIAQYASGRFGVSADYLKQGDAVEIKIG
QGAKSGMGGHLLGEKVTAEVSRIRKIPVGSDALSPARHMDIVGPEDLSMKISQLREITDWKVPIIVKFASGKVASDVKIA
AKGGADIIVVDGMQGGTGAGPDVIMEHSGIPSLAAIVEADQALKEINLREDVSLVAAGGIRSGADLAKALALGADAVYIA
TAALISIGCRVCQMCYKGNCSKGIATQDLSLRHRLDYKEMGKAVANYINAMTDEACMLVQQAGNTHITKLEKQNLRALTM
EASALTGVPMAGSENRKN

Sequences:

>Translated_498_residues
MPYRVERNPVLCKKNFGRPGCCWYLCDDRDEKICGRCFSCYNNCPHGVYEIIQGEPYPLNQEKCVGCRICLEMCPNRAIE
VNAIPQDAREAWGFPDVVEIVRKAQSASYKIRSTGALRKIPDFDDLVVIPAQVSRPPIDKYREPCGTDVVLGDRYAENPL
KLDTPVMIGAMSFGALSKEAKMALAIGSSLAGTVTNTGEGGMLPEERELADKLIAQYASGRFGVSADYLKQGDAVEIKIG
QGAKSGMGGHLLGEKVTAEVSRIRKIPVGSDALSPARHMDIVGPEDLSMKISQLREITDWKVPIIVKFASGKVASDVKIA
AKGGADIIVVDGMQGGTGAGPDVIMEHSGIPSLAAIVEADQALKEINLREDVSLVAAGGIRSGADLAKALALGADAVYIA
TAALISIGCRVCQMCYKGNCSKGIATQDLSLRHRLDYKEMGKAVANYINAMTDEACMLVQQAGNTHITKLEKQNLRALTM
EASALTGVPMAGSENRKN
>Mature_497_residues
PYRVERNPVLCKKNFGRPGCCWYLCDDRDEKICGRCFSCYNNCPHGVYEIIQGEPYPLNQEKCVGCRICLEMCPNRAIEV
NAIPQDAREAWGFPDVVEIVRKAQSASYKIRSTGALRKIPDFDDLVVIPAQVSRPPIDKYREPCGTDVVLGDRYAENPLK
LDTPVMIGAMSFGALSKEAKMALAIGSSLAGTVTNTGEGGMLPEERELADKLIAQYASGRFGVSADYLKQGDAVEIKIGQ
GAKSGMGGHLLGEKVTAEVSRIRKIPVGSDALSPARHMDIVGPEDLSMKISQLREITDWKVPIIVKFASGKVASDVKIAA
KGGADIIVVDGMQGGTGAGPDVIMEHSGIPSLAAIVEADQALKEINLREDVSLVAAGGIRSGADLAKALALGADAVYIAT
AALISIGCRVCQMCYKGNCSKGIATQDLSLRHRLDYKEMGKAVANYINAMTDEACMLVQQAGNTHITKLEKQNLRALTME
ASALTGVPMAGSENRKN

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=307, Percent_Identity=39.413680781759, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=329, Percent_Identity=39.209726443769, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320030, Length=337, Percent_Identity=35.6083086053413, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI28574881, Length=293, Percent_Identity=38.9078498293515, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24665539, Length=293, Percent_Identity=38.9078498293515, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53413; Mature: 53282

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYRVERNPVLCKKNFGRPGCCWYLCDDRDEKICGRCFSCYNNCPHGVYEIIQGEPYPLN
CCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
QEKCVGCRICLEMCPNRAIEVNAIPQDAREAWGFPDVVEIVRKAQSASYKIRSTGALRKI
CCHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCC
PDFDDLVVIPAQVSRPPIDKYREPCGTDVVLGDRYAENPLKLDTPVMIGAMSFGALSKEA
CCCCCEEEECCCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEHHHCCCCHHH
KMALAIGSSLAGTVTNTGEGGMLPEERELADKLIAQYASGRFGVSADYLKQGDAVEIKIG
HEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEC
QGAKSGMGGHLLGEKVTAEVSRIRKIPVGSDALSPARHMDIVGPEDLSMKISQLREITDW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCC
KVPIIVKFASGKVASDVKIAAKGGADIIVVDGMQGGTGAGPDVIMEHSGIPSLAAIVEAD
CCEEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHH
QALKEINLREDVSLVAAGGIRSGADLAKALALGADAVYIATAALISIGCRVCQMCYKGNC
HHHHHCCCHHCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SKGIATQDLSLRHRLDYKEMGKAVANYINAMTDEACMLVQQAGNTHITKLEKQNLRALTM
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCEEEEE
EASALTGVPMAGSENRKN
EHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PYRVERNPVLCKKNFGRPGCCWYLCDDRDEKICGRCFSCYNNCPHGVYEIIQGEPYPLN
CCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
QEKCVGCRICLEMCPNRAIEVNAIPQDAREAWGFPDVVEIVRKAQSASYKIRSTGALRKI
CCHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCC
PDFDDLVVIPAQVSRPPIDKYREPCGTDVVLGDRYAENPLKLDTPVMIGAMSFGALSKEA
CCCCCEEEECCCCCCCCHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEHHHCCCCHHH
KMALAIGSSLAGTVTNTGEGGMLPEERELADKLIAQYASGRFGVSADYLKQGDAVEIKIG
HEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEC
QGAKSGMGGHLLGEKVTAEVSRIRKIPVGSDALSPARHMDIVGPEDLSMKISQLREITDW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCC
KVPIIVKFASGKVASDVKIAAKGGADIIVVDGMQGGTGAGPDVIMEHSGIPSLAAIVEAD
CCEEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHH
QALKEINLREDVSLVAAGGIRSGADLAKALALGADAVYIATAALISIGCRVCQMCYKGNC
HHHHHCCCHHCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SKGIATQDLSLRHRLDYKEMGKAVANYINAMTDEACMLVQQAGNTHITKLEKQNLRALTM
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCEEEEE
EASALTGVPMAGSENRKN
EHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]