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The map label for this gene is cysS [H]

Identifier: 148642328

GI number: 148642328

Start: 256306

End: 257652

Strand: Reverse

Name: cysS [H]

Synonym: Msm_0268

Alternate gene names: 148642328

Gene position: 257652-256306 (Counterclockwise)

Preceding gene: 148642329

Following gene: 148642326

Centisome position: 13.9

GC content: 30.14

Gene sequence:

>1347_bases
ATGGAAATTTATTCTACTTTAACTCGTAGTAAAGAAACTTTTGAAACCATGAATGATAATAGAATTAATTTATTTGTTTG
TGGACCTACTGTTTATGATGATGCTCATATTGGTCATGGAAGAACTTACATTTCTTTTGATACAATAAAAAGATACTGGG
AATATAAAGGTTATACAGTATTTTATATTCAGAACATTACTGATGTTGATGATAAGATAATTAAAAGATCAAAAGAAACT
GGAATTCCTGCAGGTGATATAGCTAAGAAGTTTGAAAAAAGATATATTGAAGACATGGCTAAATTAAATGTCACAGGAGT
TAATTTATTTGCAAGAGCTACTGACCACATGGGCGAAATAATTGATCAGATTCAAAGATTAATTGATAAAGGTTTTGCTT
ATGAAACTGAAGACGGAGTCTATTTTGGAATAGACAATTTTAAGGAATTTGGAAAATTATCAAATAGAAACATTGAAGAA
TTGGAATCCCATAGGGAAATAGCTAAAACTACTAAGAAAAATCCGATTGATTTTGCATTATGGAAAAAAAGAGAAAATGT
AGATGAACCTGTATGGAACTCTCCATGGGGTGAAGGAAGACCTGGATGGCATATTGAAGATACAGCTATTACTGAATACT
ACTTCGGACCGCAATATGATGTTCATGGAGGGGGGCTTGACTTAATATTCCCTCATCATGAAGCAGAAATCACCCAAATG
GAAGCAGTTAGCGGAAAAAGTCCTATGGTAAATTACTGGTTACACACCGGATTTTTAAATGTTTCAGGAGAAAAAATGTC
CAAATCTTTAAATAATTTCATTACGATTCGTGAATTACTTAAAGAATATGATGCTGATGTATTCAGGTTCTTTGTATTGT
CTACTCATTACAGAAGTCCAATTGACTTTTCAAAAGAGTCACTTCATCAGTCTGAAAAAAGTTTAGATAAAATTAAAAAA
TATTATGAATCATTAGAATGTGAAATCATAGAAAATGCCACTTCTGATTATGCTCCATTAAAAATTGCTAAAGAAGAATT
CTTTAAAAGTATGGATGATGATTTCAACACTCCAAAAGCTATTGCAGCTATTTTCGGATTAATAAATGATTCCAAAAGTG
AAATTGATTCTTTAGCTGATGAAGATGTTTCAGCTATTCGTGAATTTTTAGATGATGTTGGTTTTATTTTAGGTATTGAT
TTTGTTTTAAAAGATACAGGATCTTCTGATGAATTATTAACTTTAATTTCAGATGTTAGACAAGAATTAAGAAACAACAA
ACAATATGAATTGTCTGATAAAATAAGAAATCAGCTAACTGATTTAGGTTATGAAATAAGTGATTAG

Upstream 100 bases:

>100_bases
TGACCGAGCGGCTTAGGTGCGTGGCTGCAGACCATGATACTGGGGTTCAAATCCCTAGCTTGCCTTCATTTATTAATTAT
TGCTTTTTTTGAGGTATTTT

Downstream 100 bases:

>100_bases
AGGAGATTTATAATTTATCTCCTATTTTTTCTATTTGTGCAATAGCTTCTTCTAATCCGTCACCTTTTCCAGCAGCTCCT
TTTGCAACAATGGTATCGAT

Product: cysteinyl-tRNA synthetase, CysS

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MEIYSTLTRSKETFETMNDNRINLFVCGPTVYDDAHIGHGRTYISFDTIKRYWEYKGYTVFYIQNITDVDDKIIKRSKET
GIPAGDIAKKFEKRYIEDMAKLNVTGVNLFARATDHMGEIIDQIQRLIDKGFAYETEDGVYFGIDNFKEFGKLSNRNIEE
LESHREIAKTTKKNPIDFALWKKRENVDEPVWNSPWGEGRPGWHIEDTAITEYYFGPQYDVHGGGLDLIFPHHEAEITQM
EAVSGKSPMVNYWLHTGFLNVSGEKMSKSLNNFITIRELLKEYDADVFRFFVLSTHYRSPIDFSKESLHQSEKSLDKIKK
YYESLECEIIENATSDYAPLKIAKEEFFKSMDDDFNTPKAIAAIFGLINDSKSEIDSLADEDVSAIREFLDDVGFILGID
FVLKDTGSSDELLTLISDVRQELRNNKQYELSDKIRNQLTDLGYEISD

Sequences:

>Translated_448_residues
MEIYSTLTRSKETFETMNDNRINLFVCGPTVYDDAHIGHGRTYISFDTIKRYWEYKGYTVFYIQNITDVDDKIIKRSKET
GIPAGDIAKKFEKRYIEDMAKLNVTGVNLFARATDHMGEIIDQIQRLIDKGFAYETEDGVYFGIDNFKEFGKLSNRNIEE
LESHREIAKTTKKNPIDFALWKKRENVDEPVWNSPWGEGRPGWHIEDTAITEYYFGPQYDVHGGGLDLIFPHHEAEITQM
EAVSGKSPMVNYWLHTGFLNVSGEKMSKSLNNFITIRELLKEYDADVFRFFVLSTHYRSPIDFSKESLHQSEKSLDKIKK
YYESLECEIIENATSDYAPLKIAKEEFFKSMDDDFNTPKAIAAIFGLINDSKSEIDSLADEDVSAIREFLDDVGFILGID
FVLKDTGSSDELLTLISDVRQELRNNKQYELSDKIRNQLTDLGYEISD
>Mature_448_residues
MEIYSTLTRSKETFETMNDNRINLFVCGPTVYDDAHIGHGRTYISFDTIKRYWEYKGYTVFYIQNITDVDDKIIKRSKET
GIPAGDIAKKFEKRYIEDMAKLNVTGVNLFARATDHMGEIIDQIQRLIDKGFAYETEDGVYFGIDNFKEFGKLSNRNIEE
LESHREIAKTTKKNPIDFALWKKRENVDEPVWNSPWGEGRPGWHIEDTAITEYYFGPQYDVHGGGLDLIFPHHEAEITQM
EAVSGKSPMVNYWLHTGFLNVSGEKMSKSLNNFITIRELLKEYDADVFRFFVLSTHYRSPIDFSKESLHQSEKSLDKIKK
YYESLECEIIENATSDYAPLKIAKEEFFKSMDDDFNTPKAIAAIFGLINDSKSEIDSLADEDVSAIREFLDDVGFILGID
FVLKDTGSSDELLTLISDVRQELRNNKQYELSDKIRNQLTDLGYEISD

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=496, Percent_Identity=35.2822580645161, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI303304987, Length=248, Percent_Identity=38.3064516129032, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI62240992, Length=248, Percent_Identity=38.3064516129032, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI21269877, Length=248, Percent_Identity=38.3064516129032, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI10835051, Length=248, Percent_Identity=38.3064516129032, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786737, Length=404, Percent_Identity=40.5940594059406, Blast_Score=320, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17509797, Length=246, Percent_Identity=38.6178861788618, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17509795, Length=246, Percent_Identity=38.6178861788618, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=338, Percent_Identity=32.5443786982249, Blast_Score=186, Evalue=8e-48,
Organism=Drosophila melanogaster, GI19922218, Length=393, Percent_Identity=39.440203562341, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19922364, Length=349, Percent_Identity=33.5243553008596, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 51751; Mature: 51751

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYSTLTRSKETFETMNDNRINLFVCGPTVYDDAHIGHGRTYISFDTIKRYWEYKGYTV
CCHHHHHHCCHHHHHHCCCCEEEEEEECCCEECCCCCCCCCEEEEHHHHHHHHHCCCEEE
FYIQNITDVDDKIIKRSKETGIPAGDIAKKFEKRYIEDMAKLNVTGVNLFARATDHMGEI
EEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IDQIQRLIDKGFAYETEDGVYFGIDNFKEFGKLSNRNIEELESHREIAKTTKKNPIDFAL
HHHHHHHHHCCCCEECCCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHH
WKKRENVDEPVWNSPWGEGRPGWHIEDTAITEYYFGPQYDVHGGGLDLIFPHHEAEITQM
HHCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCEEECCCCEEEEECCCCHHHHHH
EAVSGKSPMVNYWLHTGFLNVSGEKMSKSLNNFITIRELLKEYDADVFRFFVLSTHYRSP
HHHCCCCCCEEHHHECCEEECCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
IDFSKESLHQSEKSLDKIKKYYESLECEIIENATSDYAPLKIAKEEFFKSMDDDFNTPKA
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
IAAIFGLINDSKSEIDSLADEDVSAIREFLDDVGFILGIDFVLKDTGSSDELLTLISDVR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
QELRNNKQYELSDKIRNQLTDLGYEISD
HHHHCCCCEEHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEIYSTLTRSKETFETMNDNRINLFVCGPTVYDDAHIGHGRTYISFDTIKRYWEYKGYTV
CCHHHHHHCCHHHHHHCCCCEEEEEEECCCEECCCCCCCCCEEEEHHHHHHHHHCCCEEE
FYIQNITDVDDKIIKRSKETGIPAGDIAKKFEKRYIEDMAKLNVTGVNLFARATDHMGEI
EEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IDQIQRLIDKGFAYETEDGVYFGIDNFKEFGKLSNRNIEELESHREIAKTTKKNPIDFAL
HHHHHHHHHCCCCEECCCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHH
WKKRENVDEPVWNSPWGEGRPGWHIEDTAITEYYFGPQYDVHGGGLDLIFPHHEAEITQM
HHCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEECCCCEEECCCCEEEEECCCCHHHHHH
EAVSGKSPMVNYWLHTGFLNVSGEKMSKSLNNFITIRELLKEYDADVFRFFVLSTHYRSP
HHHCCCCCCEEHHHECCEEECCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
IDFSKESLHQSEKSLDKIKKYYESLECEIIENATSDYAPLKIAKEEFFKSMDDDFNTPKA
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
IAAIFGLINDSKSEIDSLADEDVSAIREFLDDVGFILGIDFVLKDTGSSDELLTLISDVR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
QELRNNKQYELSDKIRNQLTDLGYEISD
HHHHCCCCEEHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]