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The map label for this gene is thrC [H]

Identifier: 148642274

GI number: 148642274

Start: 200420

End: 201616

Strand: Reverse

Name: thrC [H]

Synonym: Msm_0214

Alternate gene names: 148642274

Gene position: 201616-200420 (Counterclockwise)

Preceding gene: 148642275

Following gene: 148642273

Centisome position: 10.88

GC content: 36.59

Gene sequence:

>1197_bases
ATGATTAGATGTGTTTCATGCGGAGAAGAATATGATAATGATGAGATAATTTACACTTGTGAAAAATGTGGATCTGTTCT
CGAACTTGTTAGTGATTTTGATGTTTCTAAAGATATCTTTGACGGCAGAAAAGATACTTTATGGAAATTTAAAGAATGCA
TACCTGTTGATGAATCTAAAATTGTTTCACTCAACGAAGGTGGAACTCCATTTTGCAAATGCGACAAATTGGGGGATGAA
TTAGGTATCAATTTATATGTTAAAGTTGAAGGGTCAAATCCGACAGGAAGTTTCAAGGACAGGGGAATGACTGTTGGTAT
GACCAAAGCTATGGAACTTGGAGTTCATACTGTTGGCTGTGCTTCAACAGGAAATACATCAGCATCTCTTGCAGCATATG
CAGCTAGAGCAGGTTTACGTTGTATTGTATTTTTACCTTCCGGAAAAGTAGCTTTAGGAAAATTGGCACAGGCTATGTTC
CACGGTGCTGAAGTAATGTCCATTAACGGAAACTTTGATGAAGCACTTGAAGCTATGACTGCTCTTGCATTAGAAAAACA
TCTTTACTTATTAAACTCAATTAACCCTTACAGATTAGAAGGTCAAAAAACAATCGGATATGAAATATTAAGGGATTTAG
GATGGCAATCTCCAGACAGGATTATTCTGCCTGTTGGAAATGCAGGAAATATTTCAGCTATCTGGAAAGGTGTTAAAGAG
TTTTACGAAGCAGGTTTTGTAGATAGTGTACCTATGATGACCGGAATTCAGGCTGAAGGTGCATGTCCAGTTACAAATGC
ATTTAAAAAACATGCTGATAGGGTTGTTCCAGTAGAAAATCCGGAAACAATAGCTACTGCAATTCGTATAGGTGCTCCTG
TAAGTGACATTAAAGCTTTAAGGGCTATTTATGAGTCTGACGGATATTCTGAAACTGTAAGTGATGAAGAGATATTGGAT
GCTCAAAAATTATTAGCTAGAAAAGAGGGAATAGGTGTTGAACCGGCTTCTGCAGCTTCAATTGCAGGTCTTAAAAAATT
ATGTGACGAAGGAGTTGTAGATAATGGAGAAACTGTAACCTGTGTTGTTACCGGTCATTTACTTAAAGATCCGAATACTG
CAATTGATGCATGTACTAAACCAAAACAAGTGGATGCAGATATTGACACTTTAAGAAAAATTTTAATGAATAAATAG

Upstream 100 bases:

>100_bases
TTGAAGTTTATTTAACTGTACCTATTGCAAATTTTATTCTTTCAATTTTTGGTTAAATTTTTATATTGATGATTATTAAA
ATAATATGTAGTGATTTTTT

Downstream 100 bases:

>100_bases
TTTTATAGCTATTTTTAATCTTTTTTCTCTTTTTTTATTTTTAATTTTATTATAAATACTTTATATTTTCATATTTTCAT
CTGATTTTTTATTTTGGTTA

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MIRCVSCGEEYDNDEIIYTCEKCGSVLELVSDFDVSKDIFDGRKDTLWKFKECIPVDESKIVSLNEGGTPFCKCDKLGDE
LGINLYVKVEGSNPTGSFKDRGMTVGMTKAMELGVHTVGCASTGNTSASLAAYAARAGLRCIVFLPSGKVALGKLAQAMF
HGAEVMSINGNFDEALEAMTALALEKHLYLLNSINPYRLEGQKTIGYEILRDLGWQSPDRIILPVGNAGNISAIWKGVKE
FYEAGFVDSVPMMTGIQAEGACPVTNAFKKHADRVVPVENPETIATAIRIGAPVSDIKALRAIYESDGYSETVSDEEILD
AQKLLARKEGIGVEPASAASIAGLKKLCDEGVVDNGETVTCVVTGHLLKDPNTAIDACTKPKQVDADIDTLRKILMNK

Sequences:

>Translated_398_residues
MIRCVSCGEEYDNDEIIYTCEKCGSVLELVSDFDVSKDIFDGRKDTLWKFKECIPVDESKIVSLNEGGTPFCKCDKLGDE
LGINLYVKVEGSNPTGSFKDRGMTVGMTKAMELGVHTVGCASTGNTSASLAAYAARAGLRCIVFLPSGKVALGKLAQAMF
HGAEVMSINGNFDEALEAMTALALEKHLYLLNSINPYRLEGQKTIGYEILRDLGWQSPDRIILPVGNAGNISAIWKGVKE
FYEAGFVDSVPMMTGIQAEGACPVTNAFKKHADRVVPVENPETIATAIRIGAPVSDIKALRAIYESDGYSETVSDEEILD
AQKLLARKEGIGVEPASAASIAGLKKLCDEGVVDNGETVTCVVTGHLLKDPNTAIDACTKPKQVDADIDTLRKILMNK
>Mature_398_residues
MIRCVSCGEEYDNDEIIYTCEKCGSVLELVSDFDVSKDIFDGRKDTLWKFKECIPVDESKIVSLNEGGTPFCKCDKLGDE
LGINLYVKVEGSNPTGSFKDRGMTVGMTKAMELGVHTVGCASTGNTSASLAAYAARAGLRCIVFLPSGKVALGKLAQAMF
HGAEVMSINGNFDEALEAMTALALEKHLYLLNSINPYRLEGQKTIGYEILRDLGWQSPDRIILPVGNAGNISAIWKGVKE
FYEAGFVDSVPMMTGIQAEGACPVTNAFKKHADRVVPVENPETIATAIRIGAPVSDIKALRAIYESDGYSETVSDEEILD
AQKLLARKEGIGVEPASAASIAGLKKLCDEGVVDNGETVTCVVTGHLLKDPNTAIDACTKPKQVDADIDTLRKILMNK

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790207, Length=304, Percent_Identity=26.9736842105263, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 42832; Mature: 42832

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRCVSCGEEYDNDEIIYTCEKCGSVLELVSDFDVSKDIFDGRKDTLWKFKECIPVDESK
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCE
IVSLNEGGTPFCKCDKLGDELGINLYVKVEGSNPTGSFKDRGMTVGMTKAMELGVHTVGC
EEEECCCCCCCEEHHHCCHHCCCEEEEEEECCCCCCCCCCCCEEEEHHHHHHHCCEEEEC
ASTGNTSASLAAYAARAGLRCIVFLPSGKVALGKLAQAMFHGAEVMSINGNFDEALEAMT
CCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
ALALEKHLYLLNSINPYRLEGQKTIGYEILRDLGWQSPDRIILPVGNAGNISAIWKGVKE
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHH
FYEAGFVDSVPMMTGIQAEGACPVTNAFKKHADRVVPVENPETIATAIRIGAPVSDIKAL
HHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHH
RAIYESDGYSETVSDEEILDAQKLLARKEGIGVEPASAASIAGLKKLCDEGVVDNGETVT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
CVVTGHLLKDPNTAIDACTKPKQVDADIDTLRKILMNK
EEEEECEECCCCHHHHHCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MIRCVSCGEEYDNDEIIYTCEKCGSVLELVSDFDVSKDIFDGRKDTLWKFKECIPVDESK
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCE
IVSLNEGGTPFCKCDKLGDELGINLYVKVEGSNPTGSFKDRGMTVGMTKAMELGVHTVGC
EEEECCCCCCCEEHHHCCHHCCCEEEEEEECCCCCCCCCCCCEEEEHHHHHHHCCEEEEC
ASTGNTSASLAAYAARAGLRCIVFLPSGKVALGKLAQAMFHGAEVMSINGNFDEALEAMT
CCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
ALALEKHLYLLNSINPYRLEGQKTIGYEILRDLGWQSPDRIILPVGNAGNISAIWKGVKE
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHH
FYEAGFVDSVPMMTGIQAEGACPVTNAFKKHADRVVPVENPETIATAIRIGAPVSDIKAL
HHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHH
RAIYESDGYSETVSDEEILDAQKLLARKEGIGVEPASAASIAGLKKLCDEGVVDNGETVT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
CVVTGHLLKDPNTAIDACTKPKQVDADIDTLRKILMNK
EEEEECEECCCCHHHHHCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]