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The map label for this gene is pyrG [H]

Identifier: 148642207

GI number: 148642207

Start: 140235

End: 141851

Strand: Reverse

Name: pyrG [H]

Synonym: Msm_0147

Alternate gene names: 148642207

Gene position: 141851-140235 (Counterclockwise)

Preceding gene: 148642208

Following gene: 148642206

Centisome position: 7.65

GC content: 36.12

Gene sequence:

>1617_bases
ATGGAGAGGATTTTTCTGACAAAGTATATTATTATAACTGGTGGGGTAGTTAGTTCTATTGGAAAAGGAATTACTTCTGC
ATCTATGGGTCGTATTTTAAGATCTTATGGTTTAAAAGTAGCGGCTATTAAAATAGATCCTTATTTGAACTGGGATTCCG
GAACATTAAACCCTTATCAGCATGGTGAAGTTTTTGTAACTAATGATGGAATGGAAACTGACTTGGATTTAGGTCATTAT
GAAAGATTTTTAGATGTTGAATTGCCTGGATTGTCCAATATCACTACAGGTAAAGTTTATAAATCTGTTATTTCTAAAGA
AAGGGAAGGCAAATTCTTGGGAGCTTGTGTACAGGTTATTCCACATATTACCAATGAAATTAAAGAAATGATTAGGTTAA
TCTCTGATAATGAGGATTATGATGTTGTTCTAATTGAGTTGGGTGGTACTGTTGGAGATATTGAAAGCCAGCCTTTCCTT
GAAGCTTTAAGGCAACTTAGAAATGAAGAAGGCCATGATAATGTCATGTTTGTTCATGTTACATTTATTCCATACTTAAA
TGCCGCCGGTGAATTCAAAACCAAACCGACACAACATTCTACAAAAGAATTAAGAAGTATGGGTATTAACCCTGATGTTA
TTGTATGCAGATCTCAGGAACCAATTGACGATGCTTTAGAAGGAAAAATAGCTCATTTCTGTGATGTGGATCAAAAAGCT
GTTGTAAACACTCCTGATGCAGGTACTATCTATGAAGTGCCACTGGTTTTAGAAGAAAATAACATTGGTGAATTGATAGT
TAATAGGATAGGACTGGATATTAAACCGGATTCCTCAAAACTTGATGACTGGCGTAAAATTGTAGAATCTCTTAAGATTG
AAAGTCCTGTTGTAAATATTGGAATTGTCGGTAAATATGTAGAGCTTGAAGATGCATATATCAGTATCAGGGAATCCCTG
CAGCATGCAGCAGCTAATGTTGGGGTAAAAGCCAACATCATCTACATCAGTTCTGATGTTGATAATTTGGATGAAGAGGT
TATGGGCAATCTCCATGGTATTCTTATTCCCGGAGGATTTGGAGAACGTGGATTTGAAGGAAAGCTTGATGCTGTAGATT
ATGCTATTGAACACAATGTTCCGTTGTTCGGTATCTGTTTGGGAATGCATTCTATTGTAACTCAGTTTGCAAGACGTAAC
GGATATCCTGAAGCTAACAGTTCAGAATTTGACAGCGAAAATCCTCACCCTGTTATTGACATGATGGAAGAACAGAAAAA
AGTTAAAAACATGGGCGGAACTATGCGTTTAGGTTCTTATGACTGTAAGCTTGTTAAAGGAACTAAAACATATGAGGCTT
ATAATGTGGAAGATATTGCAGAACGTCACAGACACAGATTTGAATTTAACAATGATTACAGGAAAGACCTGGAAGATAAA
GGTTTAGTTATTTCAGGAGTTAGTCCTGATGACTTCTTAGTGGAAATTGTTGAATTGCCAAATCATCCATGGGCTGTCGG
CTGCCAATTCCATCCGGAATTTAAATCAAGACCGAATAGGCCACATCCATTATTCAAATCATTTTTAGAAGCTATTTATG
AGTATTCTAAAAACTGA

Upstream 100 bases:

>100_bases
ACCCAAGTATAGCTACCAATTAATTTAATCTGATTTTTTCATAATTATATATAAGATGAACAACTAAATTATAAAGTATA
TAAATTTTATTTTTTATAAA

Downstream 100 bases:

>100_bases
TTCTCTTCATTTTTTTTATTTTAAAAACAATTTTACACTCTTATTTTCTAGCTAATTAATATATTTCTTAGTTTATACTA
GAATTATGGATTTTAGTATT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MERIFLTKYIIITGGVVSSIGKGITSASMGRILRSYGLKVAAIKIDPYLNWDSGTLNPYQHGEVFVTNDGMETDLDLGHY
ERFLDVELPGLSNITTGKVYKSVISKEREGKFLGACVQVIPHITNEIKEMIRLISDNEDYDVVLIELGGTVGDIESQPFL
EALRQLRNEEGHDNVMFVHVTFIPYLNAAGEFKTKPTQHSTKELRSMGINPDVIVCRSQEPIDDALEGKIAHFCDVDQKA
VVNTPDAGTIYEVPLVLEENNIGELIVNRIGLDIKPDSSKLDDWRKIVESLKIESPVVNIGIVGKYVELEDAYISIRESL
QHAAANVGVKANIIYISSDVDNLDEEVMGNLHGILIPGGFGERGFEGKLDAVDYAIEHNVPLFGICLGMHSIVTQFARRN
GYPEANSSEFDSENPHPVIDMMEEQKKVKNMGGTMRLGSYDCKLVKGTKTYEAYNVEDIAERHRHRFEFNNDYRKDLEDK
GLVISGVSPDDFLVEIVELPNHPWAVGCQFHPEFKSRPNRPHPLFKSFLEAIYEYSKN

Sequences:

>Translated_538_residues
MERIFLTKYIIITGGVVSSIGKGITSASMGRILRSYGLKVAAIKIDPYLNWDSGTLNPYQHGEVFVTNDGMETDLDLGHY
ERFLDVELPGLSNITTGKVYKSVISKEREGKFLGACVQVIPHITNEIKEMIRLISDNEDYDVVLIELGGTVGDIESQPFL
EALRQLRNEEGHDNVMFVHVTFIPYLNAAGEFKTKPTQHSTKELRSMGINPDVIVCRSQEPIDDALEGKIAHFCDVDQKA
VVNTPDAGTIYEVPLVLEENNIGELIVNRIGLDIKPDSSKLDDWRKIVESLKIESPVVNIGIVGKYVELEDAYISIRESL
QHAAANVGVKANIIYISSDVDNLDEEVMGNLHGILIPGGFGERGFEGKLDAVDYAIEHNVPLFGICLGMHSIVTQFARRN
GYPEANSSEFDSENPHPVIDMMEEQKKVKNMGGTMRLGSYDCKLVKGTKTYEAYNVEDIAERHRHRFEFNNDYRKDLEDK
GLVISGVSPDDFLVEIVELPNHPWAVGCQFHPEFKSRPNRPHPLFKSFLEAIYEYSKN
>Mature_538_residues
MERIFLTKYIIITGGVVSSIGKGITSASMGRILRSYGLKVAAIKIDPYLNWDSGTLNPYQHGEVFVTNDGMETDLDLGHY
ERFLDVELPGLSNITTGKVYKSVISKEREGKFLGACVQVIPHITNEIKEMIRLISDNEDYDVVLIELGGTVGDIESQPFL
EALRQLRNEEGHDNVMFVHVTFIPYLNAAGEFKTKPTQHSTKELRSMGINPDVIVCRSQEPIDDALEGKIAHFCDVDQKA
VVNTPDAGTIYEVPLVLEENNIGELIVNRIGLDIKPDSSKLDDWRKIVESLKIESPVVNIGIVGKYVELEDAYISIRESL
QHAAANVGVKANIIYISSDVDNLDEEVMGNLHGILIPGGFGERGFEGKLDAVDYAIEHNVPLFGICLGMHSIVTQFARRN
GYPEANSSEFDSENPHPVIDMMEEQKKVKNMGGTMRLGSYDCKLVKGTKTYEAYNVEDIAERHRHRFEFNNDYRKDLEDK
GLVISGVSPDDFLVEIVELPNHPWAVGCQFHPEFKSRPNRPHPLFKSFLEAIYEYSKN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=546, Percent_Identity=44.8717948717949, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=45.1553930530165, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=45.1553930530165, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=45.1553930530165, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=545, Percent_Identity=48.9908256880734, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI25148299, Length=599, Percent_Identity=39.2320534223706, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6322563, Length=554, Percent_Identity=41.6967509025271, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=550, Percent_Identity=40.1818181818182, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24664469, Length=551, Percent_Identity=44.1016333938294, Blast_Score=453, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21357815, Length=508, Percent_Identity=41.9291338582677, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60308; Mature: 60308

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIFLTKYIIITGGVVSSIGKGITSASMGRILRSYGLKVAAIKIDPYLNWDSGTLNPYQ
CCCEEEEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCEECCCCCCCCCCC
HGEVFVTNDGMETDLDLGHYERFLDVELPGLSNITTGKVYKSVISKEREGKFLGACVQVI
CCEEEEECCCCCCCCCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
PHITNEIKEMIRLISDNEDYDVVLIELGGTVGDIESQPFLEALRQLRNEEGHDNVMFVHV
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEE
TFIPYLNAAGEFKTKPTQHSTKELRSMGINPDVIVCRSQEPIDDALEGKIAHFCDVDQKA
EEECHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHCCCHHHHCCCCCCC
VVNTPDAGTIYEVPLVLEENNIGELIVNRIGLDIKPDSSKLDDWRKIVESLKIESPVVNI
EECCCCCCCEEEEEEEEECCCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCCCEEEE
GIVGKYVELEDAYISIRESLQHAAANVGVKANIIYISSDVDNLDEEVMGNLHGILIPGGF
EEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHCCCCEEEEECCC
GERGFEGKLDAVDYAIEHNVPLFGICLGMHSIVTQFARRNGYPEANSSEFDSENPHPVID
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
MMEEQKKVKNMGGTMRLGSYDCKLVKGTKTYEAYNVEDIAERHRHRFEFNNDYRKDLEDK
HHHHHHHHHHCCCEEEECCCCEEEEECCCCEECCCHHHHHHHHHHCCCCCCCHHHCCCCC
GLVISGVSPDDFLVEIVELPNHPWAVGCQFHPEFKSRPNRPHPLFKSFLEAIYEYSKN
CEEEECCCCHHHHHHHHHCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERIFLTKYIIITGGVVSSIGKGITSASMGRILRSYGLKVAAIKIDPYLNWDSGTLNPYQ
CCCEEEEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCEECCCCCCCCCCC
HGEVFVTNDGMETDLDLGHYERFLDVELPGLSNITTGKVYKSVISKEREGKFLGACVQVI
CCEEEEECCCCCCCCCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
PHITNEIKEMIRLISDNEDYDVVLIELGGTVGDIESQPFLEALRQLRNEEGHDNVMFVHV
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEE
TFIPYLNAAGEFKTKPTQHSTKELRSMGINPDVIVCRSQEPIDDALEGKIAHFCDVDQKA
EEECHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHCCCHHHHCCCCCCC
VVNTPDAGTIYEVPLVLEENNIGELIVNRIGLDIKPDSSKLDDWRKIVESLKIESPVVNI
EECCCCCCCEEEEEEEEECCCHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCCCEEEE
GIVGKYVELEDAYISIRESLQHAAANVGVKANIIYISSDVDNLDEEVMGNLHGILIPGGF
EEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHCCCCEEEEECCC
GERGFEGKLDAVDYAIEHNVPLFGICLGMHSIVTQFARRNGYPEANSSEFDSENPHPVID
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
MMEEQKKVKNMGGTMRLGSYDCKLVKGTKTYEAYNVEDIAERHRHRFEFNNDYRKDLEDK
HHHHHHHHHHCCCEEEECCCCEEEEECCCCEECCCHHHHHHHHHHCCCCCCCHHHCCCCC
GLVISGVSPDDFLVEIVELPNHPWAVGCQFHPEFKSRPNRPHPLFKSFLEAIYEYSKN
CEEEECCCCHHHHHHHHHCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]