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The map label for this gene is murD [H]

Identifier: 148642178

GI number: 148642178

Start: 113929

End: 115209

Strand: Reverse

Name: murD [H]

Synonym: Msm_0118

Alternate gene names: 148642178

Gene position: 115209-113929 (Counterclockwise)

Preceding gene: 148642180

Following gene: 148642177

Centisome position: 6.22

GC content: 33.26

Gene sequence:

>1281_bases
ATGAAAGCAGCAGTTATAGGTTTAGGTGTGGAAGGAAAAAAGGCAGTCAATTCTCTTTTAAGTCATGGTTGGGAGGTTTA
TGCCACTGATTTAAATAGTAATGTTGATTTATCAGATTTGGAATTGCCTATGATTTCAATGAATTTGGTCAGTGGTGGTG
AAACTGTTTCCATTGTTTCTGATAATTTAACTGTTGATTTAGGTTTTTCAAATTCAAGTGCAATTGAAGAATGTGATGCA
ATAGCTATTAGTCCCAGTATGTACGGAGGAGCTTTTGCAGATAAATTGCTTGAAAAAGGCAAACTGTTAAGCAATGTTGT
AACTAAACATAAAGACATTTTTACTATTGGCATTACAGGAACTAACGGTAAAACTACAAGTGTCCATATGTTAAAAAGTA
TTTTGGAAAATGCGGGCAAAAAAGTTTTAGTTGGCGGAAATGGTGGTGGAGGCTTTTCAGGTTACTATGATTTAATTTTA
GAAGCGTCAAATGATGATTATGATATTTTACTTGTTGAAGTTTGTGATATGACACTTGATTTTTGCAATTATTGCTTTGA
TTTCGATATGGTGGGCCTTACCAATATTGGCAATGATCATATGGATGTTCATAAGACAATAGCCAATTATAAAAATACTT
TGGTAAGATTCTTTGAAGGCAAAACGGCTTTTACAGCTTTTAATCAGGATTTCAATGCTGATTTTAAAGAAGTGGCCAGT
AAAGCTATTCCTTTCTTTGAATATGATGAAAAATTGCAAGTTTGGGGCAGATTCAATGCATTAAATGCTGGTTTGGTTGC
AGCTATAGCTACTGAGCTTAAAATACCTAAGGACATTATCATGGGATCTTTAGCTGATTTTAAAGCAGTTGAAGGAAGAA
TGAATGTTTATAAAATTAATAATGCTTCAATTTTTGTAGGTAAAACTGATAATTCAGATGCATTAACTTCAGTTTTAAAT
GAGAATGATTTCTATGCTTTATTTATAGGTACTCCAAGGGCTAATGAAGAACATAGATTAAGTATTTTGGATGAAGCGGT
TAAATATAATCCTGAAGTTATTGTTCTGTTTCCGGGATTAGATGATACTGTTGATATGGGACTTTATCGTTTAAACAGAC
TTGGTTATGAGGGTAGAATTGAGGTAGCTAACTCATTAGATGAAATAATTGCTCTTATCGCTGAATTTTCTCATGAAGAT
GCACTTTTCATTGGAGGCAACGGTCAGGAAGCTATTATTGAGATTCAAGAAAGAATAAGGCTCTTGTCTGAAAACCTTTA
G

Upstream 100 bases:

>100_bases
CAAACATTATTTCACCTTTTAAATGAGTTATGAATGTTATAATATTTATACTTGAATAACAATAGTTTATACTGTTATAA
AATATAAAGGTATTTTTTTT

Downstream 100 bases:

>100_bases
TTTGTATTCATTAGAATAAAAATATATAAATATAATAGTAACTAAATATACTATTGTTATATTATTATTGAAGGTGTTAA
ATAATATGAGTTATGAATGG

Product: peptide ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKAAVIGLGVEGKKAVNSLLSHGWEVYATDLNSNVDLSDLELPMISMNLVSGGETVSIVSDNLTVDLGFSNSSAIEECDA
IAISPSMYGGAFADKLLEKGKLLSNVVTKHKDIFTIGITGTNGKTTSVHMLKSILENAGKKVLVGGNGGGGFSGYYDLIL
EASNDDYDILLVEVCDMTLDFCNYCFDFDMVGLTNIGNDHMDVHKTIANYKNTLVRFFEGKTAFTAFNQDFNADFKEVAS
KAIPFFEYDEKLQVWGRFNALNAGLVAAIATELKIPKDIIMGSLADFKAVEGRMNVYKINNASIFVGKTDNSDALTSVLN
ENDFYALFIGTPRANEEHRLSILDEAVKYNPEVIVLFPGLDDTVDMGLYRLNRLGYEGRIEVANSLDEIIALIAEFSHED
ALFIGGNGQEAIIEIQERIRLLSENL

Sequences:

>Translated_426_residues
MKAAVIGLGVEGKKAVNSLLSHGWEVYATDLNSNVDLSDLELPMISMNLVSGGETVSIVSDNLTVDLGFSNSSAIEECDA
IAISPSMYGGAFADKLLEKGKLLSNVVTKHKDIFTIGITGTNGKTTSVHMLKSILENAGKKVLVGGNGGGGFSGYYDLIL
EASNDDYDILLVEVCDMTLDFCNYCFDFDMVGLTNIGNDHMDVHKTIANYKNTLVRFFEGKTAFTAFNQDFNADFKEVAS
KAIPFFEYDEKLQVWGRFNALNAGLVAAIATELKIPKDIIMGSLADFKAVEGRMNVYKINNASIFVGKTDNSDALTSVLN
ENDFYALFIGTPRANEEHRLSILDEAVKYNPEVIVLFPGLDDTVDMGLYRLNRLGYEGRIEVANSLDEIIALIAEFSHED
ALFIGGNGQEAIIEIQERIRLLSENL
>Mature_426_residues
MKAAVIGLGVEGKKAVNSLLSHGWEVYATDLNSNVDLSDLELPMISMNLVSGGETVSIVSDNLTVDLGFSNSSAIEECDA
IAISPSMYGGAFADKLLEKGKLLSNVVTKHKDIFTIGITGTNGKTTSVHMLKSILENAGKKVLVGGNGGGGFSGYYDLIL
EASNDDYDILLVEVCDMTLDFCNYCFDFDMVGLTNIGNDHMDVHKTIANYKNTLVRFFEGKTAFTAFNQDFNADFKEVAS
KAIPFFEYDEKLQVWGRFNALNAGLVAAIATELKIPKDIIMGSLADFKAVEGRMNVYKINNASIFVGKTDNSDALTSVLN
ENDFYALFIGTPRANEEHRLSILDEAVKYNPEVIVLFPGLDDTVDMGLYRLNRLGYEGRIEVANSLDEIIALIAEFSHED
ALFIGGNGQEAIIEIQERIRLLSENL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46551; Mature: 46551

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAVIGLGVEGKKAVNSLLSHGWEVYATDLNSNVDLSDLELPMISMNLVSGGETVSIVS
CCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEE
DNLTVDLGFSNSSAIEECDAIAISPSMYGGAFADKLLEKGKLLSNVVTKHKDIFTIGITG
CCEEEEEECCCCCHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
TNGKTTSVHMLKSILENAGKKVLVGGNGGGGFSGYYDLILEASNDDYDILLVEVCDMTLD
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHH
FCNYCFDFDMVGLTNIGNDHMDVHKTIANYKNTLVRFFEGKTAFTAFNQDFNADFKEVAS
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
KAIPFFEYDEKLQVWGRFNALNAGLVAAIATELKIPKDIIMGSLADFKAVEGRMNVYKIN
HCCCCEECCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCEEEEEEC
NASIFVGKTDNSDALTSVLNENDFYALFIGTPRANEEHRLSILDEAVKYNPEVIVLFPGL
CCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCC
DDTVDMGLYRLNRLGYEGRIEVANSLDEIIALIAEFSHEDALFIGGNGQEAIIEIQERIR
CCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
LLSENL
HHHCCC
>Mature Secondary Structure
MKAAVIGLGVEGKKAVNSLLSHGWEVYATDLNSNVDLSDLELPMISMNLVSGGETVSIVS
CCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEE
DNLTVDLGFSNSSAIEECDAIAISPSMYGGAFADKLLEKGKLLSNVVTKHKDIFTIGITG
CCEEEEEECCCCCHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
TNGKTTSVHMLKSILENAGKKVLVGGNGGGGFSGYYDLILEASNDDYDILLVEVCDMTLD
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHH
FCNYCFDFDMVGLTNIGNDHMDVHKTIANYKNTLVRFFEGKTAFTAFNQDFNADFKEVAS
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
KAIPFFEYDEKLQVWGRFNALNAGLVAAIATELKIPKDIIMGSLADFKAVEGRMNVYKIN
HCCCCEECCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCEEEEEEC
NASIFVGKTDNSDALTSVLNENDFYALFIGTPRANEEHRLSILDEAVKYNPEVIVLFPGL
CCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCC
DDTVDMGLYRLNRLGYEGRIEVANSLDEIIALIAEFSHEDALFIGGNGQEAIIEIQERIR
CCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
LLSENL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA