Click here to switch to the map view.

The map label for this gene is metG

Identifier: 148642131

GI number: 148642131

Start: 69217

End: 71199

Strand: Reverse

Name: metG

Synonym: Msm_0071

Alternate gene names: 148642131

Gene position: 71199-69217 (Counterclockwise)

Preceding gene: 148642132

Following gene: 148642130

Centisome position: 3.84

GC content: 32.73

Gene sequence:

>1983_bases
ATGTCTAAAATTTTTATTTCATGTGCGCTTCCTTATGCAAATGGACCCTGTCATTTAGGTCATATTCGTTCTACTTACTT
GCCTGCTGATATTTATGCAAGATATAATCGTATGATTGATAATGATGTTCTGTTAGTTTGTGCTACTGATGAACACGGTA
CTCCAATTGCTGTTAAAGCGGATAAAGAAAACAAAAAACCGATTGAAATTGCTAAAAGATATCATGACATGATTGTTCGT
GATGTAGAGTCAATGAATATTTCCCTGGATAACTTTACAAGAACCACTGATGATTTACATTATGAAATTGCTCAGAATTT
CTTTTTAGAATTATATAATAAGGGGTTAATTTATAAAAAGGATATTCAGCAGCTGTACTGTGAAAAGTGTAATAAGTTTT
TACCTGACCGTTATGTTGAAGGTATATGTCCTGTTTGCGGATCTGAAGCACGCGGTGACCATTGTGAAAAATGCGGAAGG
GCTTTGGAACCTACTGAACTTGATGAACCTAAATGTTTAACCTGCGGTTCCACTCCTGTTATTAAGGATACTTATCAGTA
CTTCTTTAAGTTAAGCGAGTTTGAAGATGATTTGAAAGATTATATTGATAATAATGAAAATCTTCCTGCCAATGTTAGAA
ATTATGCTAAAAATTGGCTTAAAGAAGGATTAAATGACTGGGTTTTAACAAGAGATATGGATTGGGGAATACCTTTACCT
CTTGATGAAGCTAAAGGCAAAGTACTTTATGTTTGGGTTGAAGCATTTTTGGGTTATATCTCATCAGCTGCACAATGGTC
AAGGGAAACTGGCATTAAATGGGAAGATTATTGGAATGATACTGCAATCCATTTCATTGGTAAGGATATTATTTACCATC
ATTCCATCTTTTGGCCAGGTCTTCTTAAGGCATACGGATGTAAATTGCCTGATAACATATATGCTGGAGAATTTTTATCT
TTGGAAGGAGAAAAAATGTCCACCAGTAAAAATTGGGTTGTTTGGATAGCAGATTTTGTTGATAAATTTGACCCTGATTT
ACTTAGATATTACTTAACTATAAATGCACCTTTAAACAAAGATACTGATTTCTCATGGGATGATTTCCAGAGAAGAAACA
ATGATGAATTGGCAGATGTTATAGGTAACTTTTTACATAGAACTTTTACATTTACCCATAAATTCTTTGACGGTGAAATA
CCTGAATATAAAAATCCCTCTGCAGAAGATAACAAATTCAAAGAAATCATTGAAAAACTTCCTGATACTGTTGGAGAATA
TATTTCAGATTTTGAATTTAGAGACGGTTTGCTTGAGATTTATAGGGTAGCTAAAATTGGTAATAAATATTTCAATGATC
AGGAACCATGGAAAGCTGTTAAAGAAGATATGCAAAAAGCAGCCAACTGTTTATATTTATCAAATCAACTAGCTAAAACA
TTGGCTTATGTACTTAAACCTTATATTCCAAATAAAGCTGATGCTATAGCTAAAATTATTAATCTGACCACTCCTGATGA
GTGGAAAGATGCTAAAGTCCCATTAGCTGAAGGACACAAAATAAATAAAGCTAAACCGTTATTTACAAAAATTGAAGATG
ATGTAATTAACAAGCAAAAAGAAGAATTGCAGAAAAACTTAAAAGAAAGTGAGGATGAAAATATGAGTGATTTAATTAGT
ATTGATGATTTTGACAAAGTAGTTATTAAAATTGGACAGGTAAAAGAAGCTGAAAAAATAGAAAAATCCGATAAATTATT
AAAATTACAGGTGGATATTGGAGATGAAACAAGACAAATTGTAGCAGGTCTTGCAAAACAGTATGCTCCTGAAGAATTAA
TTGACAGAAAAGTGGCTGTTGTTGTTAACTTGCAACCTGCAAAATTATTCGGAACCTTATCTGAAGGAATGATTCTTGCA
ACTGGAGAAAGTGCAGCATTATTATCTCCAGACGAATGTGAAGTCGGTGAAAGAATACAATAA

Upstream 100 bases:

>100_bases
TTTTGTTTTAAAATATTTCTTAATTAACTTCGCATATATTAATTTTATATTATATGAATGACATAATTATTAATGCTTAA
AAAAGTTAGGAGAAAAATTA

Downstream 100 bases:

>100_bases
TCTTAATTTAATTCAGTGATTTTAATGGAAGAATCTGCTCTAGTTGCAAAGGCTGAAAAATATCTCAAAGAGATTTCAAA
TGACAGTATTAGATTGGATA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSKIFISCALPYANGPCHLGHIRSTYLPADIYARYNRMIDNDVLLVCATDEHGTPIAVKADKENKKPIEIAKRYHDMIVR
DVESMNISLDNFTRTTDDLHYEIAQNFFLELYNKGLIYKKDIQQLYCEKCNKFLPDRYVEGICPVCGSEARGDHCEKCGR
ALEPTELDEPKCLTCGSTPVIKDTYQYFFKLSEFEDDLKDYIDNNENLPANVRNYAKNWLKEGLNDWVLTRDMDWGIPLP
LDEAKGKVLYVWVEAFLGYISSAAQWSRETGIKWEDYWNDTAIHFIGKDIIYHHSIFWPGLLKAYGCKLPDNIYAGEFLS
LEGEKMSTSKNWVVWIADFVDKFDPDLLRYYLTINAPLNKDTDFSWDDFQRRNNDELADVIGNFLHRTFTFTHKFFDGEI
PEYKNPSAEDNKFKEIIEKLPDTVGEYISDFEFRDGLLEIYRVAKIGNKYFNDQEPWKAVKEDMQKAANCLYLSNQLAKT
LAYVLKPYIPNKADAIAKIINLTTPDEWKDAKVPLAEGHKINKAKPLFTKIEDDVINKQKEELQKNLKESEDENMSDLIS
IDDFDKVVIKIGQVKEAEKIEKSDKLLKLQVDIGDETRQIVAGLAKQYAPEELIDRKVAVVVNLQPAKLFGTLSEGMILA
TGESAALLSPDECEVGERIQ

Sequences:

>Translated_660_residues
MSKIFISCALPYANGPCHLGHIRSTYLPADIYARYNRMIDNDVLLVCATDEHGTPIAVKADKENKKPIEIAKRYHDMIVR
DVESMNISLDNFTRTTDDLHYEIAQNFFLELYNKGLIYKKDIQQLYCEKCNKFLPDRYVEGICPVCGSEARGDHCEKCGR
ALEPTELDEPKCLTCGSTPVIKDTYQYFFKLSEFEDDLKDYIDNNENLPANVRNYAKNWLKEGLNDWVLTRDMDWGIPLP
LDEAKGKVLYVWVEAFLGYISSAAQWSRETGIKWEDYWNDTAIHFIGKDIIYHHSIFWPGLLKAYGCKLPDNIYAGEFLS
LEGEKMSTSKNWVVWIADFVDKFDPDLLRYYLTINAPLNKDTDFSWDDFQRRNNDELADVIGNFLHRTFTFTHKFFDGEI
PEYKNPSAEDNKFKEIIEKLPDTVGEYISDFEFRDGLLEIYRVAKIGNKYFNDQEPWKAVKEDMQKAANCLYLSNQLAKT
LAYVLKPYIPNKADAIAKIINLTTPDEWKDAKVPLAEGHKINKAKPLFTKIEDDVINKQKEELQKNLKESEDENMSDLIS
IDDFDKVVIKIGQVKEAEKIEKSDKLLKLQVDIGDETRQIVAGLAKQYAPEELIDRKVAVVVNLQPAKLFGTLSEGMILA
TGESAALLSPDECEVGERIQ
>Mature_659_residues
SKIFISCALPYANGPCHLGHIRSTYLPADIYARYNRMIDNDVLLVCATDEHGTPIAVKADKENKKPIEIAKRYHDMIVRD
VESMNISLDNFTRTTDDLHYEIAQNFFLELYNKGLIYKKDIQQLYCEKCNKFLPDRYVEGICPVCGSEARGDHCEKCGRA
LEPTELDEPKCLTCGSTPVIKDTYQYFFKLSEFEDDLKDYIDNNENLPANVRNYAKNWLKEGLNDWVLTRDMDWGIPLPL
DEAKGKVLYVWVEAFLGYISSAAQWSRETGIKWEDYWNDTAIHFIGKDIIYHHSIFWPGLLKAYGCKLPDNIYAGEFLSL
EGEKMSTSKNWVVWIADFVDKFDPDLLRYYLTINAPLNKDTDFSWDDFQRRNNDELADVIGNFLHRTFTFTHKFFDGEIP
EYKNPSAEDNKFKEIIEKLPDTVGEYISDFEFRDGLLEIYRVAKIGNKYFNDQEPWKAVKEDMQKAANCLYLSNQLAKTL
AYVLKPYIPNKADAIAKIINLTTPDEWKDAKVPLAEGHKINKAKPLFTKIEDDVINKQKEELQKNLKESEDENMSDLISI
DDFDKVVIKIGQVKEAEKIEKSDKLLKLQVDIGDETRQIVAGLAKQYAPEELIDRKVAVVVNLQPAKLFGTLSEGMILAT
GESAALLSPDECEVGERIQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=610, Percent_Identity=32.7868852459016, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI24308436, Length=531, Percent_Identity=25.4237288135593, Blast_Score=172, Evalue=9e-43,
Organism=Escherichia coli, GI1788432, Length=675, Percent_Identity=33.7777777777778, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1786861, Length=221, Percent_Identity=26.6968325791855, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789455, Length=99, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=575, Percent_Identity=31.4782608695652, Blast_Score=298, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI212645096, Length=508, Percent_Identity=25.9842519685039, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71992781, Length=320, Percent_Identity=26.5625, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=551, Percent_Identity=33.9382940108893, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=23.5294117647059, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24655311, Length=587, Percent_Identity=35.0936967632027, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI21358483, Length=532, Percent_Identity=27.8195488721804, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_METS3 (A5UJ98)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272644.1
- ProteinModelPortal:   A5UJ98
- STRING:   A5UJ98
- GeneID:   5216784
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0071
- eggNOG:   arNOG04371
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75895; Mature: 75764

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD; PS00018 EF_HAND_1

Important sites: BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFISCALPYANGPCHLGHIRSTYLPADIYARYNRMIDNDVLLVCATDEHGTPIAVKA
CCEEEEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEC
DKENKKPIEIAKRYHDMIVRDVESMNISLDNFTRTTDDLHYEIAQNFFLELYNKGLIYKK
CCCCCCHHHHHHHHHHHHHHHHHHCEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEHH
DIQQLYCEKCNKFLPDRYVEGICPVCGSEARGDHCEKCGRALEPTELDEPKCLTCGSTPV
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCC
IKDTYQYFFKLSEFEDDLKDYIDNNENLPANVRNYAKNWLKEGLNDWVLTRDMDWGIPLP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
LDEAKGKVLYVWVEAFLGYISSAAQWSRETGIKWEDYWNDTAIHFIGKDIIYHHSIFWPG
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHH
LLKAYGCKLPDNIYAGEFLSLEGEKMSTSKNWVVWIADFVDKFDPDLLRYYLTINAPLNK
HHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHCCCCHHHEEEEEEEECCCCC
DTDFSWDDFQRRNNDELADVIGNFLHRTFTFTHKFFDGEIPEYKNPSAEDNKFKEIIEKL
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
PDTVGEYISDFEFRDGLLEIYRVAKIGNKYFNDQEPWKAVKEDMQKAANCLYLSNQLAKT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAYVLKPYIPNKADAIAKIINLTTPDEWKDAKVPLAEGHKINKAKPLFTKIEDDVINKQK
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
EELQKNLKESEDENMSDLISIDDFDKVVIKIGQVKEAEKIEKSDKLLKLQVDIGDETRQI
HHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCHHHHH
VAGLAKQYAPEELIDRKVAVVVNLQPAKLFGTLSEGMILATGESAALLSPDECEVGERIQ
HHHHHHHCCCHHHHCCEEEEEEECCHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
SKIFISCALPYANGPCHLGHIRSTYLPADIYARYNRMIDNDVLLVCATDEHGTPIAVKA
CEEEEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEC
DKENKKPIEIAKRYHDMIVRDVESMNISLDNFTRTTDDLHYEIAQNFFLELYNKGLIYKK
CCCCCCHHHHHHHHHHHHHHHHHHCEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEHH
DIQQLYCEKCNKFLPDRYVEGICPVCGSEARGDHCEKCGRALEPTELDEPKCLTCGSTPV
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCC
IKDTYQYFFKLSEFEDDLKDYIDNNENLPANVRNYAKNWLKEGLNDWVLTRDMDWGIPLP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
LDEAKGKVLYVWVEAFLGYISSAAQWSRETGIKWEDYWNDTAIHFIGKDIIYHHSIFWPG
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHH
LLKAYGCKLPDNIYAGEFLSLEGEKMSTSKNWVVWIADFVDKFDPDLLRYYLTINAPLNK
HHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHCCCCHHHEEEEEEEECCCCC
DTDFSWDDFQRRNNDELADVIGNFLHRTFTFTHKFFDGEIPEYKNPSAEDNKFKEIIEKL
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
PDTVGEYISDFEFRDGLLEIYRVAKIGNKYFNDQEPWKAVKEDMQKAANCLYLSNQLAKT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAYVLKPYIPNKADAIAKIINLTTPDEWKDAKVPLAEGHKINKAKPLFTKIEDDVINKQK
HHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
EELQKNLKESEDENMSDLISIDDFDKVVIKIGQVKEAEKIEKSDKLLKLQVDIGDETRQI
HHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCHHHHH
VAGLAKQYAPEELIDRKVAVVVNLQPAKLFGTLSEGMILATGESAALLSPDECEVGERIQ
HHHHHHHCCCHHHHCCEEEEEEECCHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA