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The map label for this gene is adeK

Identifier: 148381553

GI number: 148381553

Start: 3857195

End: 3858481

Strand: Reverse

Name: adeK

Synonym: CBO3616

Alternate gene names: 148381553

Gene position: 3858481-3857195 (Counterclockwise)

Preceding gene: 148381554

Following gene: 148381552

Centisome position: 99.27

GC content: 31.7

Gene sequence:

>1287_bases
ATGTCAGCTTTTATAGTATTAGGGGCCCAATGGGGCGATGAAGGTAAAGGAAAAATGACTGATTATTTAGCTGAAAATGC
AGATGTAGTTGTAAGATTTCAAGGTGGTAATAACGCAGGTCATACAGTAGTTGTAGGAGAAAAGGAATATAAATTACACT
TAATACCTTCAGGTATACTGTATAATGACAAACTAAATGTAATAGGAAATGGAGTAGTTTTAGATCCAAAGGCTTTATTT
GAAGAAATAAACTATTTAGAATCTTTAGGAGTAGAAATAACACCAGATAGATTAATAATAAGTGATAGAGCTCATGTAAT
AATGCCATATCATAGGATATTAGATGGAATAAAAGAAAGAGCTAGAGGAAATAAAGATATAGGAACTACAGGAAAGGGTA
TAGGACCAAGTTATACGGATAAAATGGAAAGAAGCGGTATAAGGGTTTGCGATCTTATACATAAAGAAGTTTTTGAAGAA
AATTTGAAAGAAACTTTAGAAGTAAAAAATAAAATAATAACAGAAATATTTGGTGGAAGGGCTTTAGACTATAATGAAAT
CTATAATGAATATTTAGGATATGCAGAAAAATTAAGACCTTTTGTAAAAGATATATCTGTTATAGTTAACAAAAAAATAA
AAGATGGTAAAGAAGTGTTATTTGAAGGAGCTCAAGGAACATTACTTGATATAGATTATGGAACATATCCTTATGTAACA
TCTTCAAGCACAATAGCTGGAGGAGTTTGCATAGGAGCAGGTGTAGGGCCAACAGCTATAACTAATGCAGTAGGTATAGC
AAAAGCATATACAACAAGAGTAGGAAAAGGTCCTTTCCCAACAGAGCTTTTAGATAGCACTGGAGACTGGGTAAGAGAAA
AGGGACATGAGTTTGGAGTTACTACAGGGAGAGCAAGAAGATGTGGATGGTTAGATTTAGTTATATTAAAAACTTCAGCG
AGAATATCAGGACTTACAAGCTTTGCAGTAACAAAAATTGATACATTAGCAGGATTAGATACATTAAAAGTATGTACAGG
ATATAGACTAAATGGAGAAATTATAGATTATGTTCCTGCTAGCTTAGAAGATTTAGCAAAATGTGAACCAATATATGAAG
AATTTGAAGGTTGGGATGATAGTATAGCTAATGCTAGATGTTATGAAGATTTACCTGAAAATGCTATAAAATATTTAAAG
AAAATAGAAGATTTCACAGAAACAAAGGTATCTATAGTTTCTGTAGGTCCAAAAAGAGATCAAACTATGATGATATCAGA
AATTTAA

Upstream 100 bases:

>100_bases
TATATATTGTAGAAGTATTTTATTTAAAGCTAAAATTACATAGCATATAAATTATATTTAAATATAATAATTTTCTTAAA
ATGAAAGTGAGGAAAATAAT

Downstream 100 bases:

>100_bases
TGTATATTATAGAGCATAGTAATAAAGAATATTAATATATATAAAAAATAAATATAATATCTTTAAAGAATACTAATTTA
TTGATATAATAATAATTAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGILYNDKLNVIGNGVVLDPKALF
EEINYLESLGVEITPDRLIISDRAHVIMPYHRILDGIKERARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEE
NLKETLEVKNKIITEIFGGRALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGVTTGRARRCGWLDLVILKTSA
RISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPASLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLK
KIEDFTETKVSIVSVGPKRDQTMMISEI

Sequences:

>Translated_428_residues
MSAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGILYNDKLNVIGNGVVLDPKALF
EEINYLESLGVEITPDRLIISDRAHVIMPYHRILDGIKERARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEE
NLKETLEVKNKIITEIFGGRALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGVTTGRARRCGWLDLVILKTSA
RISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPASLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLK
KIEDFTETKVSIVSVGPKRDQTMMISEI
>Mature_427_residues
SAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGILYNDKLNVIGNGVVLDPKALFE
EINYLESLGVEITPDRLIISDRAHVIMPYHRILDGIKERARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEEN
LKETLEVKNKIITEIFGGRALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVTS
SSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGVTTGRARRCGWLDLVILKTSAR
ISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPASLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLKK
IEDFTETKVSIVSVGPKRDQTMMISEI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=46.3700234192037, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=45.5399061032864, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=43.8287153652393, Blast_Score=332, Evalue=5e-91,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=47.877358490566, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=46.1538461538462, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=46.1538461538462, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=45.852534562212, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=44.2080378250591, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CLOB1 (A7FZF9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385930.1
- ProteinModelPortal:   A7FZF9
- SMR:   A7FZF9
- STRING:   A7FZF9
- GeneID:   5397042
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3709
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   CBOT441770:CLB_3709-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47128; Mature: 46997

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGIL
CCEEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCEE
YNDKLNVIGNGVVLDPKALFEEINYLESLGVEITPDRLIISDRAHVIMPYHRILDGIKER
ECCCEEEEECCEEECHHHHHHHHHHHHHCCCEECCCEEEEECCCCEEECHHHHHHHHHHH
ARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEENLKETLEVKNKIITEIFGGR
HCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHCCCCCCC
TTGRARRCGWLDLVILKTSARISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPA
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCEEECCCC
SLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLKKIEDFTETKVSIVSVGPKRD
CHHHHHHCCHHHHHHCCCCHHHCCCHHHHHCHHHHHHHHHHHHHHHHCEEEEEEECCCCC
QTMMISEI
CEEEEECC
>Mature Secondary Structure 
SAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGIL
CEEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCEE
YNDKLNVIGNGVVLDPKALFEEINYLESLGVEITPDRLIISDRAHVIMPYHRILDGIKER
ECCCEEEEECCEEECHHHHHHHHHHHHHCCCEECCCEEEEECCCCEEECHHHHHHHHHHH
ARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEENLKETLEVKNKIITEIFGGR
HCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHCCCCCCC
TTGRARRCGWLDLVILKTSARISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPA
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCEEECCCC
SLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLKKIEDFTETKVSIVSVGPKRD
CHHHHHHCCHHHHHHCCCCHHHCCCHHHHHCHHHHHHHHHHHHHHHHCEEEEEEECCCCC
QTMMISEI
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA