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The map label for this gene is 148381540

Identifier: 148381540

GI number: 148381540

Start: 3845069

End: 3845995

Strand: Reverse

Name: 148381540

Synonym: CBO3602

Alternate gene names: NA

Gene position: 3845995-3845069 (Counterclockwise)

Preceding gene: 148381541

Following gene: 148381539

Centisome position: 98.95

GC content: 34.95

Gene sequence:

>927_bases
ATGAAGAAATCCATATTTTCTCATATGGTTGGAATAAAATATCCTATTATTCAAGGTGGAATGGCATGGATTGCTGATAG
TTCACTAGCAGCGGCAGTTTCTAATGCAGGAGGTCTTGGAATAATAACAGGAAATGCTCCAGTAGAATGGGTTAGACAAG
AAATAAGAAAGACAAAAGAATTAACAGATAAGCCCTTTGGAGTAAATATAATGCTTTTAGCAGAAACAGCAGATGAAATA
GCCCAAATGGTTTGTGATGAGGGAGTAAAAGTAGTTACTACTGGAGCAGGTAATCCAGGAAAGTATATAAAAAAATGGAA
AGAACATGGCATAATCGTAATACCTGTAGTTCCTTCTGTAGCTTTGGCTAAGAGAATGGAAAAATCAGGGGCAGATGCCA
TAATAGCAGAAGGTTGTGAATCTGGTGGTCATGTAGGGGAATTGACAACAATGACATTGATACCACAGGTAGTTGATGCT
GTAGATATACCTGTTATAGCAGCTGGAGGAATAGGTGATGGAAGAGGTATAGCAGCTAGTTTTATGTTAGGAGCAGATGC
TGTTCAAGTAGGTACTAGATTTTTAGTAGCAAAAGAATGTACTGTTCATCAAAATTATAAAAATAAAGTTATGAAAGCTA
AGGATATAGATACACAAGTTACAGGTAGACCTACAGGACACCCTGTTAGAATTATAAGAAATAGGTTATCTAGAAAGTTT
CAAATACTAGAAAAAGAAGGAGCTCCTTTAGAAGAATTTGAAAAATTAGGTAGGGGAGCATTAAGCAAAGCTGTAATAGA
GGGAGATATTGATAATGGTTCTGTTATGGCAGGTCAAATTGCAGGACTTATAAATAAGGAACAAACTTGTAGTGAAATTA
TAAATGAAATATTTGATGAAGCATATACACTATTAGATTATAAATAA

Upstream 100 bases:

>100_bases
AAAATGGTCCACAAAATTAAATTTTAAAAACAAATTAATTATAGCACATAAACATTTAATAATAAACTATCGATTTAAGA
TTCTAGGAGGAATTAAAACT

Downstream 100 bases:

>100_bases
TATTTATTTTGGAGGATATTATGTCAAAGATAGCTTTTATATTCTCAGGTCAAGGAGCTCAATATGTAGGTATGGGGAAA
GATTTATATAAAAATATTCC

Product: oxidoreductase, 2-nitropropane dioxygenase family

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKKSIFSHMVGIKYPIIQGGMAWIADSSLAAAVSNAGGLGIITGNAPVEWVRQEIRKTKELTDKPFGVNIMLLAETADEI
AQMVCDEGVKVVTTGAGNPGKYIKKWKEHGIIVIPVVPSVALAKRMEKSGADAIIAEGCESGGHVGELTTMTLIPQVVDA
VDIPVIAAGGIGDGRGIAASFMLGADAVQVGTRFLVAKECTVHQNYKNKVMKAKDIDTQVTGRPTGHPVRIIRNRLSRKF
QILEKEGAPLEEFEKLGRGALSKAVIEGDIDNGSVMAGQIAGLINKEQTCSEIINEIFDEAYTLLDYK

Sequences:

>Translated_308_residues
MKKSIFSHMVGIKYPIIQGGMAWIADSSLAAAVSNAGGLGIITGNAPVEWVRQEIRKTKELTDKPFGVNIMLLAETADEI
AQMVCDEGVKVVTTGAGNPGKYIKKWKEHGIIVIPVVPSVALAKRMEKSGADAIIAEGCESGGHVGELTTMTLIPQVVDA
VDIPVIAAGGIGDGRGIAASFMLGADAVQVGTRFLVAKECTVHQNYKNKVMKAKDIDTQVTGRPTGHPVRIIRNRLSRKF
QILEKEGAPLEEFEKLGRGALSKAVIEGDIDNGSVMAGQIAGLINKEQTCSEIINEIFDEAYTLLDYK
>Mature_308_residues
MKKSIFSHMVGIKYPIIQGGMAWIADSSLAAAVSNAGGLGIITGNAPVEWVRQEIRKTKELTDKPFGVNIMLLAETADEI
AQMVCDEGVKVVTTGAGNPGKYIKKWKEHGIIVIPVVPSVALAKRMEKSGADAIIAEGCESGGHVGELTTMTLIPQVVDA
VDIPVIAAGGIGDGRGIAASFMLGADAVQVGTRFLVAKECTVHQNYKNKVMKAKDIDTQVTGRPTGHPVRIIRNRLSRKF
QILEKEGAPLEEFEKLGRGALSKAVIEGDIDNGSVMAGQIAGLINKEQTCSEIINEIFDEAYTLLDYK

Specific function: Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=166, Percent_Identity=29.5180722891566, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 32913; Mature: 32913

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSIFSHMVGIKYPIIQGGMAWIADSSLAAAVSNAGGLGIITGNAPVEWVRQEIRKTKE
CCHHHHHHHHCCCCHHHHCCHHHHCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
LTDKPFGVNIMLLAETADEIAQMVCDEGVKVVTTGAGNPGKYIKKWKEHGIIVIPVVPSV
HCCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCEEEEECCCHH
ALAKRMEKSGADAIIAEGCESGGHVGELTTMTLIPQVVDAVDIPVIAAGGIGDGRGIAAS
HHHHHHHHCCCCCEEHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHH
FMLGADAVQVGTRFLVAKECTVHQNYKNKVMKAKDIDTQVTGRPTGHPVRIIRNRLSRKF
HHHCCHHHHHHHHHEEEHHHHHHCCHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHH
QILEKEGAPLEEFEKLGRGALSKAVIEGDIDNGSVMAGQIAGLINKEQTCSEIINEIFDE
HHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHH
AYTLLDYK
HHHHHCCC
>Mature Secondary Structure
MKKSIFSHMVGIKYPIIQGGMAWIADSSLAAAVSNAGGLGIITGNAPVEWVRQEIRKTKE
CCHHHHHHHHCCCCHHHHCCHHHHCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHH
LTDKPFGVNIMLLAETADEIAQMVCDEGVKVVTTGAGNPGKYIKKWKEHGIIVIPVVPSV
HCCCCCCEEEEEEECCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCEEEEECCCHH
ALAKRMEKSGADAIIAEGCESGGHVGELTTMTLIPQVVDAVDIPVIAAGGIGDGRGIAAS
HHHHHHHHCCCCCEEHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHH
FMLGADAVQVGTRFLVAKECTVHQNYKNKVMKAKDIDTQVTGRPTGHPVRIIRNRLSRKF
HHHCCHHHHHHHHHEEEHHHHHHCCHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHHH
QILEKEGAPLEEFEKLGRGALSKAVIEGDIDNGSVMAGQIAGLINKEQTCSEIINEIFDE
HHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHH
AYTLLDYK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]