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The map label for this gene is glmU

Identifier: 148381485

GI number: 148381485

Start: 3790641

End: 3792014

Strand: Reverse

Name: glmU

Synonym: CBO3545

Alternate gene names: 148381485

Gene position: 3792014-3790641 (Counterclockwise)

Preceding gene: 148381486

Following gene: 148381484

Centisome position: 97.56

GC content: 31.22

Gene sequence:

>1374_bases
ATGTATAATTGTGCTATAATATTGGCTGCAGGCAAAGGAAAAAGAATGAAATCTTCTATGCCTAAAGTCGTACACAAGGT
TTGTGGAAAAGAAATGGTAAACCACGTCATTGATAACGTTAGAAAAGCTAATATAAAGGATGTAAACTTGGTAATAGGAA
AAGGATCGGAAACAGTTAAAGAGCATACAAAAGATAGAAATGTTACTTATTCTATGCAAGAAGAACAACTAGGAACTGGC
CATGCTGTTATTTGTGCAGAGGAATTTTTAAAAGATAAAAAAGGTACAGTAGCTATATTTACAGGAGATGCTCCTTTAAT
CACCAATGAAACTATACAACAATTATTTGAATTTCATAATAGTGGGAAATATGCAGCTACTTTAATTTCTTCTACTGTAC
AAGATCCAACAGGATATGGGCGAATTATAAGAGAAGCATCTGGGGAAGTAAAGAAAATAGTTGAACATAAAGATTGCAAT
GAAGAAGAGCTTAAGGTAAATGAAATTAACAGTGGAATGTATTGCTTTGATATAGAAGTTTTATTAAATAGTTTAAAAAA
TCTTAATAATGATAATTCTCAAGGAGAATATTATTTAACAGATGTTATAGAGATAACAAAAAAATCTGGAGATAAAGTTG
GTGCTATAGTTGTACCTTATGAAGAGATCATGGGTGTAAATTCAAGAGTTCAACTTTCAGAAGCAGAGATAGTTATGAGA
AAAAGAATAAATCATAAACATATGGTAAATGGAGTAACATTTATAGACTGTGAAAGTACATATATAGATGTTGATGTAGA
AATAGGAAATGATACTATAATATATCCAGGATGCGTTATCCAGGGAAATACAAGAATAAAAGAAGAATGTACGTTATATT
CTAATTCAAGAATATGTAATAGCGTAATAGGATCAGGAGTTATAGTTGAGAATTCTGTCATATTAGAAAGTCATGTGGGA
GAAGGTACTACTGTAGGACCATTTGCTTATATAAGGCCAGAAACTAAAATAGGTAAATCTGCAAGAATAGGGGATTTCGT
AGAAATAAAAAAATCAACTATTGGAGACAACACAAAGGTATCACACCTTACTTATATAGGGGATGCAGAAGTAGGCAGCA
AGTGCAATTTTGGATGTGGAACTGTAGTAGTAAATTATGATGGACAAAAGAAACAAAAAACTATTATTGGCAACAATGCT
TTTATAGGATGTAACACAAATTTGATTTCACCAGTTAAAGTTAATGACAATACATATATTGCAGCAGGATCTACAATTAC
TAAGGAAGTACCAGAAGGTTCTTTAGCAATTGCTAGATCAAAGCAGATCAATAAAGAAGGCTGGTTAGATAAAAAAGGAT
TATTAAAGAAATAA

Upstream 100 bases:

>100_bases
AATAGGTTGAAAATAATTTTTATATAAAATATAATATTATAAGATGTTTTATTAAATGAAATAAAAGTATATCATAATTA
AACCATAAAGAGGTGCTATA

Downstream 100 bases:

>100_bases
AATTTAAGGAGGACTATATAATGATAACCCATGGAAAAGGTATTAAAATTTTTTCAGGTAATTCTCACCCGAAATTAGCT
CAAGATATTGCTAGTATTTT

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MYNCAIILAAGKGKRMKSSMPKVVHKVCGKEMVNHVIDNVRKANIKDVNLVIGKGSETVKEHTKDRNVTYSMQEEQLGTG
HAVICAEEFLKDKKGTVAIFTGDAPLITNETIQQLFEFHNSGKYAATLISSTVQDPTGYGRIIREASGEVKKIVEHKDCN
EEELKVNEINSGMYCFDIEVLLNSLKNLNNDNSQGEYYLTDVIEITKKSGDKVGAIVVPYEEIMGVNSRVQLSEAEIVMR
KRINHKHMVNGVTFIDCESTYIDVDVEIGNDTIIYPGCVIQGNTRIKEECTLYSNSRICNSVIGSGVIVENSVILESHVG
EGTTVGPFAYIRPETKIGKSARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGSKCNFGCGTVVVNYDGQKKQKTIIGNNA
FIGCNTNLISPVKVNDNTYIAAGSTITKEVPEGSLAIARSKQINKEGWLDKKGLLKK

Sequences:

>Translated_457_residues
MYNCAIILAAGKGKRMKSSMPKVVHKVCGKEMVNHVIDNVRKANIKDVNLVIGKGSETVKEHTKDRNVTYSMQEEQLGTG
HAVICAEEFLKDKKGTVAIFTGDAPLITNETIQQLFEFHNSGKYAATLISSTVQDPTGYGRIIREASGEVKKIVEHKDCN
EEELKVNEINSGMYCFDIEVLLNSLKNLNNDNSQGEYYLTDVIEITKKSGDKVGAIVVPYEEIMGVNSRVQLSEAEIVMR
KRINHKHMVNGVTFIDCESTYIDVDVEIGNDTIIYPGCVIQGNTRIKEECTLYSNSRICNSVIGSGVIVENSVILESHVG
EGTTVGPFAYIRPETKIGKSARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGSKCNFGCGTVVVNYDGQKKQKTIIGNNA
FIGCNTNLISPVKVNDNTYIAAGSTITKEVPEGSLAIARSKQINKEGWLDKKGLLKK
>Mature_457_residues
MYNCAIILAAGKGKRMKSSMPKVVHKVCGKEMVNHVIDNVRKANIKDVNLVIGKGSETVKEHTKDRNVTYSMQEEQLGTG
HAVICAEEFLKDKKGTVAIFTGDAPLITNETIQQLFEFHNSGKYAATLISSTVQDPTGYGRIIREASGEVKKIVEHKDCN
EEELKVNEINSGMYCFDIEVLLNSLKNLNNDNSQGEYYLTDVIEITKKSGDKVGAIVVPYEEIMGVNSRVQLSEAEIVMR
KRINHKHMVNGVTFIDCESTYIDVDVEIGNDTIIYPGCVIQGNTRIKEECTLYSNSRICNSVIGSGVIVENSVILESHVG
EGTTVGPFAYIRPETKIGKSARIGDFVEIKKSTIGDNTKVSHLTYIGDAEVGSKCNFGCGTVVVNYDGQKKQKTIIGNNA
FIGCNTNLISPVKVNDNTYIAAGSTITKEVPEGSLAIARSKQINKEGWLDKKGLLKK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=446, Percent_Identity=41.2556053811659, Blast_Score=337, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI133931050, Length=380, Percent_Identity=24.7368421052632, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=358, Percent_Identity=24.5810055865922, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_CLOB1 (A7FPK2)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385860.1
- ProteinModelPortal:   A7FPK2
- SMR:   A7FPK2
- STRING:   A7FPK2
- GeneID:   5397709
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3626
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14354
- BioCyc:   CBOT441770:CLB_3626-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR001451
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00132 Hexapep; PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 50131; Mature: 50131

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 362-362 BINDING 73-73 BINDING 140-140 BINDING 155-155 BINDING 170-170 BINDING 386-386 BINDING 422-422 BINDING 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNCAIILAAGKGKRMKSSMPKVVHKVCGKEMVNHVIDNVRKANIKDVNLVIGKGSETVK
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHH
EHTKDRNVTYSMQEEQLGTGHAVICAEEFLKDKKGTVAIFTGDAPLITNETIQQLFEFHN
HHHCCCCEEEEECHHHCCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHC
SGKYAATLISSTVQDPTGYGRIIREASGEVKKIVEHKDCNEEELKVNEINSGMYCFDIEV
CCCEEEHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHEEEEECCCCEEEEEHHH
LLNSLKNLNNDNSQGEYYLTDVIEITKKSGDKVGAIVVPYEEIMGVNSRVQLSEAEIVMR
HHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEEECHHHHHCCCCEEEEHHHHHHHH
KRINHKHMVNGVTFIDCESTYIDVDVEIGNDTIIYPGCVIQGNTRIKEECTLYSNSRICN
HHCCHHHHHCCEEEEECCCEEEEEEEEECCCEEEECCEEEECCCCCCCCEEEECCCHHHH
SVIGSGVIVENSVILESHVGEGTTVGPFAYIRPETKIGKSARIGDFVEIKKSTIGDNTKV
HHHCCCEEEECCEEEEECCCCCCEECCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEE
SHLTYIGDAEVGSKCNFGCGTVVVNYDGQKKQKTIIGNNAFIGCNTNLISPVKVNDNTYI
EEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCEEEECCCCEECEEEECCCEEE
AAGSTITKEVPEGSLAIARSKQINKEGWLDKKGLLKK
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYNCAIILAAGKGKRMKSSMPKVVHKVCGKEMVNHVIDNVRKANIKDVNLVIGKGSETVK
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHH
EHTKDRNVTYSMQEEQLGTGHAVICAEEFLKDKKGTVAIFTGDAPLITNETIQQLFEFHN
HHHCCCCEEEEECHHHCCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHC
SGKYAATLISSTVQDPTGYGRIIREASGEVKKIVEHKDCNEEELKVNEINSGMYCFDIEV
CCCEEEHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHEEEEECCCCEEEEEHHH
LLNSLKNLNNDNSQGEYYLTDVIEITKKSGDKVGAIVVPYEEIMGVNSRVQLSEAEIVMR
HHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEEECHHHHHCCCCEEEEHHHHHHHH
KRINHKHMVNGVTFIDCESTYIDVDVEIGNDTIIYPGCVIQGNTRIKEECTLYSNSRICN
HHCCHHHHHCCEEEEECCCEEEEEEEEECCCEEEECCEEEECCCCCCCCEEEECCCHHHH
SVIGSGVIVENSVILESHVGEGTTVGPFAYIRPETKIGKSARIGDFVEIKKSTIGDNTKV
HHHCCCEEEECCEEEEECCCCCCEECCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEE
SHLTYIGDAEVGSKCNFGCGTVVVNYDGQKKQKTIIGNNAFIGCNTNLISPVKVNDNTYI
EEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCEEEECCCCEECEEEECCCEEE
AAGSTITKEVPEGSLAIARSKQINKEGWLDKKGLLKK
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA