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The map label for this gene is pth

Identifier: 148381480

GI number: 148381480

Start: 3785499

End: 3786068

Strand: Reverse

Name: pth

Synonym: CBO3540

Alternate gene names: 148381480

Gene position: 3786068-3785499 (Counterclockwise)

Preceding gene: 148381481

Following gene: 148381479

Centisome position: 97.41

GC content: 27.37

Gene sequence:

>570_bases
ATGTATTTAGTAGTTGGATTAGGAAATATAGGAAAAGAATATAAAAAGACTAGACATAATATAGGTTTTGATGTAGTTGA
CATTATAGCTGAGAAGTATAATATTGAAATAAATAGGCAAAAATTTAAAGGAAGTTATGGTGAAGGAAGAATAGGAAATG
AAAAGATAATATTATTAAAACCTAGTACATATATGAATTTAAGTGGAGAAAGTGTTATAGAAGCAGCTAATTTTTATAAA
ATAGATAAAGAAAATATAATAGTGATATATGATGATATGAGTATAGACATAGGAAAATTAAGAGTTAGAAGCAAGGGAAG
TGCAGGTGGACATAATGGTATAAAAAATATTATACAACATTTAAATTCAGATATTTTTCCTAGAGTTAGAGTAGGCATTG
GACAGCCTGATGAAAATGTAGTAAACTATGTTTTGGGAAAATTTTCAAAAGATGAAAGGGAAATTATAGAAAAAGTACTA
GCTATGTCAGCTAAAGCATGTATAAGTATAGTAGAAGATGGAGTGACAGAGGCTATGAATAAGTACAATGGAGTAAAAAT
AGAAGTATAA

Upstream 100 bases:

>100_bases
TATAATAAATAGTAAAGGGACCTAATAAGTCTCTTTATTATTTATTTATTATAGTATAATATTCTTTAGTGATATTAATA
TTATAATTAGGAGTGAAGGT

Downstream 100 bases:

>100_bases
AACAATAATTATAGGGTGGTTGTTAATATGAGACTAGAAGGTTTAGTATCACCTTTGAGTTTAAGTGAAGATTTTAAAAG
TTTATTAAATAATATAAATT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MYLVVGLGNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEGRIGNEKIILLKPSTYMNLSGESVIEAANFYK
IDKENIIVIYDDMSIDIGKLRVRSKGSAGGHNGIKNIIQHLNSDIFPRVRVGIGQPDENVVNYVLGKFSKDEREIIEKVL
AMSAKACISIVEDGVTEAMNKYNGVKIEV

Sequences:

>Translated_189_residues
MYLVVGLGNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEGRIGNEKIILLKPSTYMNLSGESVIEAANFYK
IDKENIIVIYDDMSIDIGKLRVRSKGSAGGHNGIKNIIQHLNSDIFPRVRVGIGQPDENVVNYVLGKFSKDEREIIEKVL
AMSAKACISIVEDGVTEAMNKYNGVKIEV
>Mature_189_residues
MYLVVGLGNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEGRIGNEKIILLKPSTYMNLSGESVIEAANFYK
IDKENIIVIYDDMSIDIGKLRVRSKGSAGGHNGIKNIIQHLNSDIFPRVRVGIGQPDENVVNYVLGKFSKDEREIIEKVL
AMSAKACISIVEDGVTEAMNKYNGVKIEV

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=164, Percent_Identity=34.1463414634146, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1787455, Length=190, Percent_Identity=38.9473684210526, Blast_Score=121, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321983, Length=177, Percent_Identity=26.5536723163842, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_CLOB1 (A7FPJ7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385855.1
- ProteinModelPortal:   A7FPJ7
- SMR:   A7FPJ7
- STRING:   A7FPJ7
- GeneID:   5398121
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3621
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   CBOT441770:CLB_3621-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21113; Mature: 21113

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLVVGLGNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEGRIGNEKIILLK
CEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEHHHHCCCCCCCCCCCCEEEEEC
PSTYMNLSGESVIEAANFYKIDKENIIVIYDDMSIDIGKLRVRSKGSAGGHNGIKNIIQH
CCCEECCCCHHHHHHHHHEEECCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHH
LNSDIFPRVRVGIGQPDENVVNYVLGKFSKDEREIIEKVLAMSAKACISIVEDGVTEAMN
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYNGVKIEV
HCCCEEEEC
>Mature Secondary Structure
MYLVVGLGNIGKEYKKTRHNIGFDVVDIIAEKYNIEINRQKFKGSYGEGRIGNEKIILLK
CEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCEEEHHHHCCCCCCCCCCCCEEEEEC
PSTYMNLSGESVIEAANFYKIDKENIIVIYDDMSIDIGKLRVRSKGSAGGHNGIKNIIQH
CCCEECCCCHHHHHHHHHEEECCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHH
LNSDIFPRVRVGIGQPDENVVNYVLGKFSKDEREIIEKVLAMSAKACISIVEDGVTEAMN
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYNGVKIEV
HCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA