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The map label for this gene is cysS

Identifier: 148381443

GI number: 148381443

Start: 3728614

End: 3730011

Strand: Reverse

Name: cysS

Synonym: CBO3501

Alternate gene names: 148381443

Gene position: 3730011-3728614 (Counterclockwise)

Preceding gene: 148381444

Following gene: 148381442

Centisome position: 95.96

GC content: 28.18

Gene sequence:

>1398_bases
ATGAAGGTTTACAATACTTTAACTAATAAAAAAGAAGAGTTTCTTACGTTAGTACCAGGAGAAGTTAAAATGTATGTTTG
TGGTCCTACGGTATATAATTTTTTCCATATAGGGAATGCTAGAACTTTTGTAGTTTTTGATACTATAAGAAGGTATTTAG
AATATAGGGGTTATAAAGTGAAATTTATTCAAAATTTTACTGACATAGATGATAAGATGATAAAAAGAGCTAATGAAGAA
GGATCGACGGTAAAAGAATTAGGAGATAGATTTATAAAAGAATATTATAAAGATGCAGATGATTTAAATATTGAAAGGGC
AACTAAAAATCCAAGAGCAACTGAATTCATGGAGGAAATAATAAAATTTGTGAGTGACCTAATAGAAAAAGGGTATGCTT
ATGAAATAGATGGTGACGTATATTTTAGTACTAAAAAATTTAATAGTTATGGTAAATTATCAGGACAAAATTTAGAAGAA
TTACAATTAGGAGCAAGAATAAATGTAGATGAAAGAAAAAAAGATCCTATGGACTTCGCTATATGGAAAAGCCAAAAGCC
GGGAGAACCTGCTTGGGAAAGTCCTTGGGGAATGGGAAGACCAGGTTGGCATATAGAATGCTCATGCATGGCTTATAATT
TGTTAGGAGAGACAATAGATATACATGCAGGAGGATCAGATTTATCATTTCCACATCATGAAAATGAAATAGCTCAAAGT
GAGGCAAGAACAGGAAAGCAATTTGCAAAGTATTGGTTACATTCAGCTTTTGTTAATGTAAATAATCAAAAGATGTCTAA
ATCATTAAATAATTTCTTTACAGCAAGAGAAATATTAGAAAAATATGATGCAGATGTTTTAAGAATGTTTATGTTATCAG
GACATTATAGAACTCAAATAAACTTTAGTATGGAGTTATTAGATTCAACTAAAGCAGCTTTAGATAGATTGTATAATTCT
ATAAATAATTTAGAAAATTTATTAGATGAAGTAAAGAATGAAGAATTAAGAGATGAAGAATTAGAATACAAAAATGAATT
ACAAAAATATAAAGAAAAATATATAGAAAAAATGGATGATGATTTTAATACAGCAGATGCTATATCTATAATATTTGATT
TAATAAGAGATGTTAATACAAATGTTACTATAGAATCATCAAAAGAACTAGTTAAATATACATTAGATTTAATAAGAGAA
TTAGGAAGCCCTTTAGGAATATTACAGGAATCAACTAAAGCTAGTTTAGAAGAAGAGATTGAAAAACTTATAGAAGAAAG
ACAAAAAGCTAGAAAAGAAAAAAATTGGGCATTAGCAGATAAAATTAGAGATAATTTGAAGGAAAGAGGCATTGTTTTGG
AAGATACACCACAGGGAATAAGATGGAAGCAAATATAA

Upstream 100 bases:

>100_bases
GAACAAGGAGATAAGTTATTGAAACAACTTAGCGAAAAAGGGTATAATATACAAACAAATATATGTATGTAATAAATTTA
GTAAAATAGGAGGTAAATAG

Downstream 100 bases:

>100_bases
GATATAAGTACTAGTGGATTAATAAAAATAGAATGAGTGGAGAGTAATATGGATTTTAGTTTATTTCAAAATAAGTTTAC
TATTAAGGATGGAAAAACAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKVYNTLTNKKEEFLTLVPGEVKMYVCGPTVYNFFHIGNARTFVVFDTIRRYLEYRGYKVKFIQNFTDIDDKMIKRANEE
GSTVKELGDRFIKEYYKDADDLNIERATKNPRATEFMEEIIKFVSDLIEKGYAYEIDGDVYFSTKKFNSYGKLSGQNLEE
LQLGARINVDERKKDPMDFAIWKSQKPGEPAWESPWGMGRPGWHIECSCMAYNLLGETIDIHAGGSDLSFPHHENEIAQS
EARTGKQFAKYWLHSAFVNVNNQKMSKSLNNFFTAREILEKYDADVLRMFMLSGHYRTQINFSMELLDSTKAALDRLYNS
INNLENLLDEVKNEELRDEELEYKNELQKYKEKYIEKMDDDFNTADAISIIFDLIRDVNTNVTIESSKELVKYTLDLIRE
LGSPLGILQESTKASLEEEIEKLIEERQKARKEKNWALADKIRDNLKERGIVLEDTPQGIRWKQI

Sequences:

>Translated_465_residues
MKVYNTLTNKKEEFLTLVPGEVKMYVCGPTVYNFFHIGNARTFVVFDTIRRYLEYRGYKVKFIQNFTDIDDKMIKRANEE
GSTVKELGDRFIKEYYKDADDLNIERATKNPRATEFMEEIIKFVSDLIEKGYAYEIDGDVYFSTKKFNSYGKLSGQNLEE
LQLGARINVDERKKDPMDFAIWKSQKPGEPAWESPWGMGRPGWHIECSCMAYNLLGETIDIHAGGSDLSFPHHENEIAQS
EARTGKQFAKYWLHSAFVNVNNQKMSKSLNNFFTAREILEKYDADVLRMFMLSGHYRTQINFSMELLDSTKAALDRLYNS
INNLENLLDEVKNEELRDEELEYKNELQKYKEKYIEKMDDDFNTADAISIIFDLIRDVNTNVTIESSKELVKYTLDLIRE
LGSPLGILQESTKASLEEEIEKLIEERQKARKEKNWALADKIRDNLKERGIVLEDTPQGIRWKQI
>Mature_465_residues
MKVYNTLTNKKEEFLTLVPGEVKMYVCGPTVYNFFHIGNARTFVVFDTIRRYLEYRGYKVKFIQNFTDIDDKMIKRANEE
GSTVKELGDRFIKEYYKDADDLNIERATKNPRATEFMEEIIKFVSDLIEKGYAYEIDGDVYFSTKKFNSYGKLSGQNLEE
LQLGARINVDERKKDPMDFAIWKSQKPGEPAWESPWGMGRPGWHIECSCMAYNLLGETIDIHAGGSDLSFPHHENEIAQS
EARTGKQFAKYWLHSAFVNVNNQKMSKSLNNFFTAREILEKYDADVLRMFMLSGHYRTQINFSMELLDSTKAALDRLYNS
INNLENLLDEVKNEELRDEELEYKNELQKYKEKYIEKMDDDFNTADAISIIFDLIRDVNTNVTIESSKELVKYTLDLIRE
LGSPLGILQESTKASLEEEIEKLIEERQKARKEKNWALADKIRDNLKERGIVLEDTPQGIRWKQI

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=480, Percent_Identity=33.9583333333333, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI21269877, Length=412, Percent_Identity=32.0388349514563, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI303304987, Length=412, Percent_Identity=32.0388349514563, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI62240992, Length=412, Percent_Identity=32.0388349514563, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI10835051, Length=412, Percent_Identity=32.0388349514563, Blast_Score=199, Evalue=6e-51,
Organism=Escherichia coli, GI1786737, Length=477, Percent_Identity=48.4276729559748, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17509797, Length=358, Percent_Identity=34.9162011173184, Blast_Score=181, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17509795, Length=358, Percent_Identity=34.9162011173184, Blast_Score=181, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=361, Percent_Identity=37.1191135734072, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI19922218, Length=500, Percent_Identity=34, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI19922364, Length=344, Percent_Identity=34.593023255814, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_CLOB1 (A7FZ91)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385818.1
- ProteinModelPortal:   A7FZ91
- SMR:   A7FZ91
- STRING:   A7FZ91
- GeneID:   5395808
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3561
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   CBOT441770:CLB_3561-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54316; Mature: 54316

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 267-267

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYNTLTNKKEEFLTLVPGEVKMYVCGPTVYNFFHIGNARTFVVFDTIRRYLEYRGYKV
CCCCHHHCCCHHHHEEECCCCEEEEEECCHHHHHEEECCCCEEEEHHHHHHHHHHCCCEE
KFIQNFTDIDDKMIKRANEEGSTVKELGDRFIKEYYKDADDLNIERATKNPRATEFMEEI
EEECCHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
IKFVSDLIEKGYAYEIDGDVYFSTKKFNSYGKLSGQNLEELQLGARINVDERKKDPMDFA
HHHHHHHHHCCCEEEECCCEEEEEHHCCCCCCCCCCCHHHHHCCCCCCCHHCCCCCCCEE
IWKSQKPGEPAWESPWGMGRPGWHIECSCMAYNLLGETIDIHAGGSDLSFPHHENEIAQS
EECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHH
EARTGKQFAKYWLHSAFVNVNNQKMSKSLNNFFTAREILEKYDADVLRMFMLSGHYRTQI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NFSMELLDSTKAALDRLYNSINNLENLLDEVKNEELRDEELEYKNELQKYKEKYIEKMDD
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DFNTADAISIIFDLIRDVNTNVTIESSKELVKYTLDLIRELGSPLGILQESTKASLEEEI
CCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EKLIEERQKARKEKNWALADKIRDNLKERGIVLEDTPQGIRWKQI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKVYNTLTNKKEEFLTLVPGEVKMYVCGPTVYNFFHIGNARTFVVFDTIRRYLEYRGYKV
CCCCHHHCCCHHHHEEECCCCEEEEEECCHHHHHEEECCCCEEEEHHHHHHHHHHCCCEE
KFIQNFTDIDDKMIKRANEEGSTVKELGDRFIKEYYKDADDLNIERATKNPRATEFMEEI
EEECCHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
IKFVSDLIEKGYAYEIDGDVYFSTKKFNSYGKLSGQNLEELQLGARINVDERKKDPMDFA
HHHHHHHHHCCCEEEECCCEEEEEHHCCCCCCCCCCCHHHHHCCCCCCCHHCCCCCCCEE
IWKSQKPGEPAWESPWGMGRPGWHIECSCMAYNLLGETIDIHAGGSDLSFPHHENEIAQS
EECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHH
EARTGKQFAKYWLHSAFVNVNNQKMSKSLNNFFTAREILEKYDADVLRMFMLSGHYRTQI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NFSMELLDSTKAALDRLYNSINNLENLLDEVKNEELRDEELEYKNELQKYKEKYIEKMDD
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DFNTADAISIIFDLIRDVNTNVTIESSKELVKYTLDLIRELGSPLGILQESTKASLEEEI
CCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EKLIEERQKARKEKNWALADKIRDNLKERGIVLEDTPQGIRWKQI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA