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The map label for this gene is truA [H]

Identifier: 148381387

GI number: 148381387

Start: 3688696

End: 3689436

Strand: Reverse

Name: truA [H]

Synonym: CBO3447

Alternate gene names: 148381387

Gene position: 3689436-3688696 (Counterclockwise)

Preceding gene: 148381388

Following gene: 148381386

Centisome position: 94.92

GC content: 28.07

Gene sequence:

>741_bases
GTGAAAAATATAAAATTGAAACTTGAGTATGATGGAGCAAATTATTGTGGATGGCAAAAACAAAAAAATGAAAAGTTTAT
TACGTTACAAGGTACTTTAGAAAAAGCTATAAGTAATATAACTAAAGAAAAAATAGAAGTTATAGGAGCCAGTAGAACAG
ATTCGGGGGTCCATGCTAGAGGATATATATGTAATTTTTTTACAAACACTAAAATACCACCTGAAAACTTACAAAAAGTA
ATTAATAATATTTTACCACCAGATATAGTAGTTTTAAGTTCAGAGGAAGCTAGTAGTGAGTTCCATTCTAGATTTTGTAG
TAAGGGCAAGACTTATGAATATACAGTTCTAAATAGCTCTAAGCCTATAGCCATAGGCAGAAATTATATTTTTCAGTTTA
AAGATAAACTCAATATAGATAATATGAAGATGGCATCAAGATATTTTATAGGAACACATGATTTCTCTGCTTTTAAAACA
AAGGGAAGTAGTATAAAAACTTCAGTAAGAACTATAAGTAAATTTGAAATAAATAAAAAGGGTGATTTTATAAAGTTTAT
AATTACAGGAGATGGTTTTCTTTATAACATGGTAAGAATAATAGTAGGTACATTGATAGAAATTGGACTAAATAAAAGAG
AGCCAGAATATATAAAGTATGTTATAAAATCAAAGGATAGAACAAAAGCAGGGAAATGTGTGCCTTCATCTGGACTGTGT
TTAAAGGAAGTATTTTATTAA

Upstream 100 bases:

>100_bases
CTAGAATGAAAGAACTTTCTCTTTCGTCAAGAGATTTTGTAGCAGCAGCATTTACTTCTATTTTAGTTATACTATCTATT
TGGAGTAGGATGTGGACTTA

Downstream 100 bases:

>100_bases
TACTATTGACACACCGGGCAGATTGTATTATAATAACTTTGTTTGTATAGTATAATAGATTCACTAGCCCCGGATCTTTA
TATAGAAGAACGTTTTATGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHARGYICNFFTNTKIPPENLQKV
INNILPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSSKPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKT
KGSSIKTSVRTISKFEINKKGDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRTKAGKCVPSSGLC
LKEVFY

Sequences:

>Translated_246_residues
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHARGYICNFFTNTKIPPENLQKV
INNILPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSSKPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKT
KGSSIKTSVRTISKFEINKKGDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRTKAGKCVPSSGLC
LKEVFY
>Mature_246_residues
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHARGYICNFFTNTKIPPENLQKV
INNILPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSSKPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKT
KGSSIKTSVRTISKFEINKKGDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRTKAGKCVPSSGLC
LKEVFY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=27.7153558052434, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI199559785, Length=261, Percent_Identity=24.5210727969349, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=31.6872427983539, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27967; Mature: 27967

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAR
CCCEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
GYICNFFTNTKIPPENLQKVINNILPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSS
CEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCC
KPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKTKGSSIKTSVRTISKFEINKK
CCEEECCCEEEEECCCCCCCCEEHHHEEEEECCCCHHHHCCCCCHHHHHHHHHHEEECCC
GDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRTKAGKCVPSSGLC
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
LKEVFY
HHHHCC
>Mature Secondary Structure
MKNIKLKLEYDGANYCGWQKQKNEKFITLQGTLEKAISNITKEKIEVIGASRTDSGVHAR
CCCEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
GYICNFFTNTKIPPENLQKVINNILPPDIVVLSSEEASSEFHSRFCSKGKTYEYTVLNSS
CEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEECCC
KPIAIGRNYIFQFKDKLNIDNMKMASRYFIGTHDFSAFKTKGSSIKTSVRTISKFEINKK
CCEEECCCEEEEECCCCCCCCEEHHHEEEEECCCCHHHHCCCCCHHHHHHHHHHEEECCC
GDFIKFIITGDGFLYNMVRIIVGTLIEIGLNKREPEYIKYVIKSKDRTKAGKCVPSSGLC
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
LKEVFY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]