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The map label for this gene is buk2 [H]

Identifier: 148381368

GI number: 148381368

Start: 3667528

End: 3668622

Strand: Reverse

Name: buk2 [H]

Synonym: CBO3428

Alternate gene names: 148381368

Gene position: 3668622-3667528 (Counterclockwise)

Preceding gene: 148381369

Following gene: 148381367

Centisome position: 94.38

GC content: 30.96

Gene sequence:

>1095_bases
ATGAGTGGTAATGATCTTATATTAGTAATTAATCCAGGATCTACATCTACTAAAATTGCTTTATTTAATAAAGAAAATCC
TATAATTACAGATAATTTATTTCATTCACTAGAGGAAATAAATAAATATGATTCAATATATGAACAAAAAGGTATGAGAG
AAAAAATAATAATGAAATGGCTAGAAGAGAAGGGTGTGGATTTAAATAAACTTGTTGCTATAGTAGGAAGAGGTGGCTTA
CTACGCCCTATGCCAGGTGGTACTTATAAGGTAACAAAAAAAATGCTAGAAGATTTAAAAATAGGTTATCAAGGACAGCA
TGCTTCTAATCTTGGAGGGGTTATAGCCTATGATATTTCTAAAAAATTAAATATACCTTCGTTTATAGTGGATCCAGTAG
CAGTAGACGAAATATTAGATGAAGCTAGAATATCTGGTATGCCTGAAATAAAAAGAAGGTCTTTAGTTCATGCCTTAAAT
ATAAAGGCTGTAACAAGAAAAGTTTGTAAGAAGATTGATAAAAACTTTTATAATAGTTCCTTTGTAGTAGCACACTTGGG
TGGAGGTATATCAGTATGTCCAATTGAAAATGGAAAAATATTAGATGTAAATAATGCTAATGAAGAAGGACCATTTTCTC
CAGAAAGGACAGGAAGTTTACCTGTAGGAGATTTAATAAAAATAGCTTATAGCAGTAAATATCCTTATAAAGAACTAAAG
AAAAAGATTATAGGTAAAGGTGGGCTAATAGCATACCTAGATACAAATGATGGAAGACTTGTAGATAGGATGATTGAGGA
AGGTAATAAAAAAGCTGAACTTATTTTAGAAGCTATGGCGTATCAAATTGCAAAGGAAATAGGTTCTATGGCCACAGTAT
TAAATGGTAATGTAGATGCTATAATCCTTACAGGAGGCCTAGCTTATAATAAAAGATTGACTACCTGGATAAAAGAAAGA
GTTGAGTTTATATCTCCAATTGAATTGGTACCTGGAGAAGAGGAGATGTTAGCTTTAGTTGAAGGGGCAATAAGAATTTT
AAATAAAGAAGAAAATGCTAAAATTTATGAGGAAGAGGTGTGTTTTAATGATTAA

Upstream 100 bases:

>100_bases
AAACTTTTAAAAAAAATATTGCGTTGTTCATGAATTTTGATAAAATATAAGTTAAAGAATAGTATAAGCTAAAGAATAAT
ATAAATATGGGAGATTTAAA

Downstream 100 bases:

>100_bases
AAGTTTTGATGAAATACTACAAAAGGCTAAGAGCCAAGAAAGAAGAACTATATCTGTAGCGGTTGCTCAAGATAAACATG
TATTAGAAGCTATAAAGGAT

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSGNDLILVINPGSTSTKIALFNKENPIITDNLFHSLEEINKYDSIYEQKGMREKIIMKWLEEKGVDLNKLVAIVGRGGL
LRPMPGGTYKVTKKMLEDLKIGYQGQHASNLGGVIAYDISKKLNIPSFIVDPVAVDEILDEARISGMPEIKRRSLVHALN
IKAVTRKVCKKIDKNFYNSSFVVAHLGGGISVCPIENGKILDVNNANEEGPFSPERTGSLPVGDLIKIAYSSKYPYKELK
KKIIGKGGLIAYLDTNDGRLVDRMIEEGNKKAELILEAMAYQIAKEIGSMATVLNGNVDAIILTGGLAYNKRLTTWIKER
VEFISPIELVPGEEEMLALVEGAIRILNKEENAKIYEEEVCFND

Sequences:

>Translated_364_residues
MSGNDLILVINPGSTSTKIALFNKENPIITDNLFHSLEEINKYDSIYEQKGMREKIIMKWLEEKGVDLNKLVAIVGRGGL
LRPMPGGTYKVTKKMLEDLKIGYQGQHASNLGGVIAYDISKKLNIPSFIVDPVAVDEILDEARISGMPEIKRRSLVHALN
IKAVTRKVCKKIDKNFYNSSFVVAHLGGGISVCPIENGKILDVNNANEEGPFSPERTGSLPVGDLIKIAYSSKYPYKELK
KKIIGKGGLIAYLDTNDGRLVDRMIEEGNKKAELILEAMAYQIAKEIGSMATVLNGNVDAIILTGGLAYNKRLTTWIKER
VEFISPIELVPGEEEMLALVEGAIRILNKEENAKIYEEEVCFND
>Mature_363_residues
SGNDLILVINPGSTSTKIALFNKENPIITDNLFHSLEEINKYDSIYEQKGMREKIIMKWLEEKGVDLNKLVAIVGRGGLL
RPMPGGTYKVTKKMLEDLKIGYQGQHASNLGGVIAYDISKKLNIPSFIVDPVAVDEILDEARISGMPEIKRRSLVHALNI
KAVTRKVCKKIDKNFYNSSFVVAHLGGGISVCPIENGKILDVNNANEEGPFSPERTGSLPVGDLIKIAYSSKYPYKELKK
KIIGKGGLIAYLDTNDGRLVDRMIEEGNKKAELILEAMAYQIAKEIGSMATVLNGNVDAIILTGGLAYNKRLTTWIKERV
EFISPIELVPGEEEMLALVEGAIRILNKEENAKIYEEEVCFND

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=340, Percent_Identity=23.5294117647059, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 40327; Mature: 40196

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNDLILVINPGSTSTKIALFNKENPIITDNLFHSLEEINKYDSIYEQKGMREKIIMKW
CCCCCEEEEECCCCCCEEEEEEECCCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LEEKGVDLNKLVAIVGRGGLLRPMPGGTYKVTKKMLEDLKIGYQGQHASNLGGVIAYDIS
HHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
KKLNIPSFIVDPVAVDEILDEARISGMPEIKRRSLVHALNIKAVTRKVCKKIDKNFYNSS
CCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVVAHLGGGISVCPIENGKILDVNNANEEGPFSPERTGSLPVGDLIKIAYSSKYPYKELK
EEEEEECCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KKIIGKGGLIAYLDTNDGRLVDRMIEEGNKKAELILEAMAYQIAKEIGSMATVLNGNVDA
HHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IILTGGLAYNKRLTTWIKERVEFISPIELVPGEEEMLALVEGAIRILNKEENAKIYEEEV
EEEECCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHH
CFND
CCCC
>Mature Secondary Structure 
SGNDLILVINPGSTSTKIALFNKENPIITDNLFHSLEEINKYDSIYEQKGMREKIIMKW
CCCCEEEEECCCCCCEEEEEEECCCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LEEKGVDLNKLVAIVGRGGLLRPMPGGTYKVTKKMLEDLKIGYQGQHASNLGGVIAYDIS
HHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
KKLNIPSFIVDPVAVDEILDEARISGMPEIKRRSLVHALNIKAVTRKVCKKIDKNFYNSS
CCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVVAHLGGGISVCPIENGKILDVNNANEEGPFSPERTGSLPVGDLIKIAYSSKYPYKELK
EEEEEECCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
KKIIGKGGLIAYLDTNDGRLVDRMIEEGNKKAELILEAMAYQIAKEIGSMATVLNGNVDA
HHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IILTGGLAYNKRLTTWIKERVEFISPIELVPGEEEMLALVEGAIRILNKEENAKIYEEEV
EEEECCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHH
CFND
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]