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The map label for this gene is proS1

Identifier: 148381286

GI number: 148381286

Start: 3574592

End: 3576028

Strand: Reverse

Name: proS1

Synonym: CBO3344

Alternate gene names: 148381286

Gene position: 3576028-3574592 (Counterclockwise)

Preceding gene: 148381287

Following gene: 148381285

Centisome position: 92.0

GC content: 33.96

Gene sequence:

>1437_bases
ATGGCAGGAGATAAAAAATTTGTTGAAGATATTACACCAATGGATGAAGATTTTGCACAATGGTATACGGATATAGTAAA
AAAGGCAGAGCTTGCTGATTACTCAAGTATCAGAGGATGTATGATTATTCGTCCTAATGGCTATGGAATTTGGGAAAATA
TACAGAAATATGTAGATACAAAACTTAAAGAGTATGGACATGAAAATGTATCTATGCCAATATTTATACCTGAAAACTTA
TTACAAAAAGAAAAAGATCACGTTGAAGGATTTGCACCTGAAGTTGCATGGGTAACTCATGGTGGAGATGACGAATTAGC
TGAAAGATTATGTGTTCGTCCAACATCAGAAACATTGTTCTGTGAACACTATGCTAAGATTGTTCAATCTTATAAAGATT
TACCAAAGCTTTATAACCAATGGTGTTCAGTTGTAAGATGGGAAAAGACAACTAGACCTTTTTTAAGAACAACTGAATTC
TTATGGCAAGAAGGTCATACAATTCATGAAACAAAAGAAGAAGCAGAATCTCATTCACTTAAGATACTAAACATGTATTC
TAGATTATGTGAAGATATGTTAGCAATGCCAGTGGTTATGGGTAAAAAGACAGATAAGGAAAAATTTGCTGGTGCAGATG
ATACGTACACAATTGAAAGTTTAATGCATGATGGAAAGGCACTGCAAGCAGGTACTTCACACTATCTTGGACAAAATTTC
TCAAAGGCATTTGCAATTCAGTTCTCAGATAGAAATGGGAAGTTAGATTATCCACACTATACTACTTGGGCAGTAACTAC
AAGATTAATTGGAGCAATAATAATGGTCCATGGTGATAATAGTGGTCTAAAATTACCACCAAGAATTGCACCAACTCAAG
CGGTGATTATTCCTGTTGCTCAACATAAAGAAGGAGTACTGGAAAAAGCGAAAGAATTAAAGGAAAAATTGGCTAAGGTT
GTAAGAGTTAAACTTGATGATAGCGATAAGATGCCAGGTTGGAAGTATAGTGAATATGAAATGAAGGGTATCCCACTAAG
AATAGAAATTGGGCCAAAGGATATAGAAAAAAACCAAGCTGTGTTAGTAAGAAGAGACAATAGAGAAAAAACAATAGTTT
CATTGGACGAAATTGAAATAAAGGTTCAAGAGATGTTAGATATTATCCATAATTCAATGCTAGAAGAAGCTAAAAAAACT
AGAGATGAAAAAACTTATGTAGCAACAAATATGGAAGAATTTGAAGATACTATTGAAAATAAACCAGGTTTTATTAAAGC
AATGTGGTGTGGAGATAGAGCTTGTGAAGATAAGATAAGAGAGGTAACTGGCGCTACATCTCGTTGTATGCCATTTGAAC
AAGAGGTTGTTTCAGACACTTGTGTATGTTGTGGTAAAAAAGCTAAAAATTTAGTTTACTGGGGAAGAGCATATTAA

Upstream 100 bases:

>100_bases
ATGTTAACAAGGGTGGTACCACCGACACAACTTCGGTCCCTGTATAGGGATTAGGAGTTTTTTTATTTACAAAAAAATAA
ATTTTAGGGAGGAATTTAAT

Downstream 100 bases:

>100_bases
TTACAAGTTATTTAATTAATAGATTTTATAAAATAAATATTAGAAAATTAAAGGGTATAGGGGAATCAATGATTTTTCTA
TACCTTTTATATAGTGGGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYGIWENIQKYVDTKLKEYGHENVSMPIFIPENL
LQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEF
LWQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAKELKEKLAKV
VRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKT
RDEKTYVATNMEEFEDTIENKPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY

Sequences:

>Translated_478_residues
MAGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYGIWENIQKYVDTKLKEYGHENVSMPIFIPENL
LQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEF
LWQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAKELKEKLAKV
VRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKT
RDEKTYVATNMEEFEDTIENKPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
>Mature_477_residues
AGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYGIWENIQKYVDTKLKEYGHENVSMPIFIPENLL
QKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFL
WQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNFS
KAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAKELKEKLAKVV
RVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTR
DEKTYVATNMEEFEDTIENKPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=44.2190669371197, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI1786392, Length=159, Percent_Identity=27.6729559748428, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=39.7959183673469, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71991346, Length=358, Percent_Identity=43.854748603352, Blast_Score=343, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6321808, Length=497, Percent_Identity=45.4728370221328, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24649468, Length=494, Percent_Identity=42.7125506072874, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=494, Percent_Identity=42.7125506072874, Blast_Score=400, Evalue=1e-112,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_CLOB1 (A7FYU0)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385663.1
- ProteinModelPortal:   A7FYU0
- SMR:   A7FYU0
- STRING:   A7FYU0
- GeneID:   5394593
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3402
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   CBOT441770:CLB_3402-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54921; Mature: 54790

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYGIWENIQKYVDT
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHHH
KLKEYGHENVSMPIFIPENLLQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLF
HHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
CEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETKEEAESHSL
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHCCCHHHHCCCC
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVA
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEEEEECH
QHKEGVLEKAKELKEKLAKVVRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQA
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCE
VLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTRDEKTYVATNMEEFEDTIEN
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCC
KPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
CCCEEEEEECCCHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHCCCEEEECCCC
>Mature Secondary Structure 
AGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYGIWENIQKYVDT
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCHHHHHHHHHHH
KLKEYGHENVSMPIFIPENLLQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLF
HHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
CEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETKEEAESHSL
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHCCCHHHHCCCC
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVA
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEEEEECH
QHKEGVLEKAKELKEKLAKVVRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQA
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCE
VLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTRDEKTYVATNMEEFEDTIEN
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCC
KPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
CCCEEEEEECCCHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA