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The map label for this gene is aegA [H]

Identifier: 148381247

GI number: 148381247

Start: 3514866

End: 3516251

Strand: Reverse

Name: aegA [H]

Synonym: CBO3304

Alternate gene names: 148381247

Gene position: 3516251-3514866 (Counterclockwise)

Preceding gene: 148381248

Following gene: 148381246

Centisome position: 90.46

GC content: 35.64

Gene sequence:

>1386_bases
ATGGATAGAATGAAAAGGGTGTCAGTAAAAGAACAAGATCCTAATAAGAGAATTACAAACTTTGATGAGGTTTGTTTAGG
ATATACAGAAGAAGACGCTATAGAAGAAGCCAAAAGATGTCTTCATTGTAAAAAGCCTATGTGTGTTGGAAAGTGTCCTG
TAAGCGTTAATATACCAGAATTTATAGAACATATTAAAGAAGGAGAGTTTGAAAAAGCAGCTAAGGCAATAGCAAAAGAT
AGTGCATTGCCAGCAGTTTGTGGAAGAGTGTGTCCTCAAGAAACTCAATGTGAAGGTAAATGTGTTCTTGGAATAAAGGG
AGAACCAGTAGCTATAGGCAAATTGGAAAGGTTTGTAGCAGATTGGTCAAGAAAAAATAACGTGGATTTAAGTAGTACAG
AAGAAAAGAAAAATAAAAAAGTAGCTGTAATAGGAAGTGGTCCTGCAGGGCTTACCTGTGCAGGAGATTTGGCTAAAAAA
GGATATGATGTTACTATATTTGAAGCTTTACACGAACCAGGTGGCGTATTAGTCTATGGTATACCAGAATTTAGATTACC
CAAGGATACAGTAGTAAAGCATGAAATAGACAATGTTAAAAAATTAGGAGTTAAAATAGAAACTAATGTAATAGTTGGAA
GAACTGTAACTATTGATGATTTAGTAGAAGAAGAAGATTTTCAAGCTATATTTATAGGTTCCGGAGCAGGACTCCCAAAA
TTTATGGGGATACCAGGAGAAAATTTAAACGGAGTATTCTCTGCTAATGAATTTTTAACAAGAACTAATCTTATGAAAGC
CTTTAAAGAAGAATATGATACGCCTATTAAAGTAGGAGAAAAAGTTGCTGTAGTTGGTGGTGGAAATGTTGCTATGGATG
CTGCAAGAACTGCCAAAAGACTTGGAGCCGAAGTATATATAGTTTATAGAAGATCAGAATCAGAACTACCAGCTAGAGTA
GAAGAGGTACATCATGCAAAGGAAGAAGGAATAAAACTTAATTTGCTAACTAATCCTGTAGAGATTTTAGGGGATGAAAA
TGGTTGGGTAAAAGGAATGAAGTGTATTAAAATGGAACTAGGAGAGCCAGATCAATCAGGAAGAAGAAGACCTATTGAAA
TACCAGGTTCAGAATTTACTATAGAGTTAGATAGTGTTATTATGTCTTTAGGTACATCTCCAAATCCACTTATAGCATCC
ACAACAGAGGGATTAGAAAGTACAAAGCGAGGCTGTATAGTAGCTGAGGAAGAAACAGGAGCTACTACAAAGGTAGGAGT
ATTTGCAGGTGGAGATGCAGTAACAGGTGCTGCTACAGTTATATTGGCTATGGGAGCAGGTAAAAAAGCTGCTAAAGCTA
TAGATGAATATTTAAGTGATAAATAA

Upstream 100 bases:

>100_bases
GACAACAAATGTATAAAACTGAAGAAGGAAAGAAATTATTAGAAGATCAAGAAAAAGCAGAAGGACATGTATGCCATGTA
GGATTGGATGGTGATAAATA

Downstream 100 bases:

>100_bases
TATTATCTTATAATAAATATTGACAAATATGTATAATTAGTTATAACCTATAAGCTGTACTTTATATTTAAAGTACAGCT
TATTTATTTTCAAATTTTAG

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MDRMKRVSVKEQDPNKRITNFDEVCLGYTEEDAIEEAKRCLHCKKPMCVGKCPVSVNIPEFIEHIKEGEFEKAAKAIAKD
SALPAVCGRVCPQETQCEGKCVLGIKGEPVAIGKLERFVADWSRKNNVDLSSTEEKKNKKVAVIGSGPAGLTCAGDLAKK
GYDVTIFEALHEPGGVLVYGIPEFRLPKDTVVKHEIDNVKKLGVKIETNVIVGRTVTIDDLVEEEDFQAIFIGSGAGLPK
FMGIPGENLNGVFSANEFLTRTNLMKAFKEEYDTPIKVGEKVAVVGGGNVAMDAARTAKRLGAEVYIVYRRSESELPARV
EEVHHAKEEGIKLNLLTNPVEILGDENGWVKGMKCIKMELGEPDQSGRRRPIEIPGSEFTIELDSVIMSLGTSPNPLIAS
TTEGLESTKRGCIVAEEETGATTKVGVFAGGDAVTGAATVILAMGAGKKAAKAIDEYLSDK

Sequences:

>Translated_461_residues
MDRMKRVSVKEQDPNKRITNFDEVCLGYTEEDAIEEAKRCLHCKKPMCVGKCPVSVNIPEFIEHIKEGEFEKAAKAIAKD
SALPAVCGRVCPQETQCEGKCVLGIKGEPVAIGKLERFVADWSRKNNVDLSSTEEKKNKKVAVIGSGPAGLTCAGDLAKK
GYDVTIFEALHEPGGVLVYGIPEFRLPKDTVVKHEIDNVKKLGVKIETNVIVGRTVTIDDLVEEEDFQAIFIGSGAGLPK
FMGIPGENLNGVFSANEFLTRTNLMKAFKEEYDTPIKVGEKVAVVGGGNVAMDAARTAKRLGAEVYIVYRRSESELPARV
EEVHHAKEEGIKLNLLTNPVEILGDENGWVKGMKCIKMELGEPDQSGRRRPIEIPGSEFTIELDSVIMSLGTSPNPLIAS
TTEGLESTKRGCIVAEEETGATTKVGVFAGGDAVTGAATVILAMGAGKKAAKAIDEYLSDK
>Mature_461_residues
MDRMKRVSVKEQDPNKRITNFDEVCLGYTEEDAIEEAKRCLHCKKPMCVGKCPVSVNIPEFIEHIKEGEFEKAAKAIAKD
SALPAVCGRVCPQETQCEGKCVLGIKGEPVAIGKLERFVADWSRKNNVDLSSTEEKKNKKVAVIGSGPAGLTCAGDLAKK
GYDVTIFEALHEPGGVLVYGIPEFRLPKDTVVKHEIDNVKKLGVKIETNVIVGRTVTIDDLVEEEDFQAIFIGSGAGLPK
FMGIPGENLNGVFSANEFLTRTNLMKAFKEEYDTPIKVGEKVAVVGGGNVAMDAARTAKRLGAEVYIVYRRSESELPARV
EEVHHAKEEGIKLNLLTNPVEILGDENGWVKGMKCIKMELGEPDQSGRRRPIEIPGSEFTIELDSVIMSLGTSPNPLIAS
TTEGLESTKRGCIVAEEETGATTKVGVFAGGDAVTGAATVILAMGAGKKAAKAIDEYLSDK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=470, Percent_Identity=32.3404255319149, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI237757300, Length=106, Percent_Identity=38.6792452830189, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788811, Length=455, Percent_Identity=38.4615384615385, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1789606, Length=464, Percent_Identity=36.8534482758621, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI87082180, Length=448, Percent_Identity=37.7232142857143, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1788468, Length=441, Percent_Identity=34.0136054421769, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789243, Length=417, Percent_Identity=33.0935251798561, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=477, Percent_Identity=28.7211740041929, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI71984108, Length=450, Percent_Identity=31.5555555555556, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17543860, Length=178, Percent_Identity=31.4606741573034, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=30.625, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28574881, Length=483, Percent_Identity=30.4347826086957, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665539, Length=483, Percent_Identity=30.4347826086957, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665547, Length=483, Percent_Identity=30.4347826086957, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665543, Length=483, Percent_Identity=30.4347826086957, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24640763, Length=449, Percent_Identity=32.0712694877506, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI18858217, Length=449, Percent_Identity=32.0712694877506, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 49797; Mature: 49797

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRMKRVSVKEQDPNKRITNFDEVCLGYTEEDAIEEAKRCLHCKKPMCVGKCPVSVNIPE
CCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHH
FIEHIKEGEFEKAAKAIAKDSALPAVCGRVCPQETQCEGKCVLGIKGEPVAIGKLERFVA
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHHHH
DWSRKNNVDLSSTEEKKNKKVAVIGSGPAGLTCAGDLAKKGYDVTIFEALHEPGGVLVYG
HHCCCCCCCCCCCHHCCCCEEEEEECCCCCCEECCHHHHCCCCEEEEHHHCCCCCEEEEC
IPEFRLPKDTVVKHEIDNVKKLGVKIETNVIVGRTVTIDDLVEEEDFQAIFIGSGAGLPK
CCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEHHHHHCCCCCCEEEEECCCCCCH
FMGIPGENLNGVFSANEFLTRTNLMKAFKEEYDTPIKVGEKVAVVGGGNVAMDAARTAKR
HCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEHHHHHHHHH
LGAEVYIVYRRSESELPARVEEVHHAKEEGIKLNLLTNPVEILGDENGWVKGMKCIKMEL
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCHHEECCCCCCCCCEEEEEEEC
GEPDQSGRRRPIEIPGSEFTIELDSVIMSLGTSPNPLIASTTEGLESTKRGCIVAEEETG
CCCCCCCCCCCEECCCCEEEEEHHHHHHHHCCCCCCEEEECHHHHHHHCCCEEEEECCCC
ATTKVGVFAGGDAVTGAATVILAMGAGKKAAKAIDEYLSDK
CEEEEEEEECCCCHHHHEEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRMKRVSVKEQDPNKRITNFDEVCLGYTEEDAIEEAKRCLHCKKPMCVGKCPVSVNIPE
CCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHH
FIEHIKEGEFEKAAKAIAKDSALPAVCGRVCPQETQCEGKCVLGIKGEPVAIGKLERFVA
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCEEHHHHHHHHH
DWSRKNNVDLSSTEEKKNKKVAVIGSGPAGLTCAGDLAKKGYDVTIFEALHEPGGVLVYG
HHCCCCCCCCCCCHHCCCCEEEEEECCCCCCEECCHHHHCCCCEEEEHHHCCCCCEEEEC
IPEFRLPKDTVVKHEIDNVKKLGVKIETNVIVGRTVTIDDLVEEEDFQAIFIGSGAGLPK
CCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEHHHHHCCCCCCEEEEECCCCCCH
FMGIPGENLNGVFSANEFLTRTNLMKAFKEEYDTPIKVGEKVAVVGGGNVAMDAARTAKR
HCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEHHHHHHHHH
LGAEVYIVYRRSESELPARVEEVHHAKEEGIKLNLLTNPVEILGDENGWVKGMKCIKMEL
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCEEEEEECCCHHEECCCCCCCCCEEEEEEEC
GEPDQSGRRRPIEIPGSEFTIELDSVIMSLGTSPNPLIASTTEGLESTKRGCIVAEEETG
CCCCCCCCCCCEECCCCEEEEEHHHHHHHHCCCCCCEEEECHHHHHHHCCCEEEEECCCC
ATTKVGVFAGGDAVTGAATVILAMGAGKKAAKAIDEYLSDK
CEEEEEEEECCCCHHHHEEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]