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The map label for this gene is fldB [H]

Identifier: 148381233

GI number: 148381233

Start: 3496590

End: 3497813

Strand: Reverse

Name: fldB [H]

Synonym: CBO3290

Alternate gene names: 148381233

Gene position: 3497813-3496590 (Counterclockwise)

Preceding gene: 148381234

Following gene: 148381232

Centisome position: 89.99

GC content: 33.91

Gene sequence:

>1224_bases
ATGAGTGATAGAAATAAGGAAGTAAAAGAAAAAAAAGCAAAGCATTATCTTAGAGAGATTACTGCAAAGCATTATAAAGA
GGCTCTCGAAGCAAAAGAAAGGGGAGAAAAAGTTGGTTGGTGTGCATCCAACTTCCCACAAGAAATAGCTACAACATTGG
GGATAAAGGTTGTTTATCCAGAAAATCATGCAGCAGCTGTAGCAGCTAGAGGAAATGGACAAAACATGTGTGAACATGCT
GAGGCTATGGGATTTTCTAATGATGTATGTGGTTATGCAAGAGTAAATTTAGCTGTTATGGACATAGGTCATAGTGAAGA
TCAACCAATACCTATGCCAGATTTTGTACTTTGCTGTAATAACATTTGTAATCAAATGATTAAATGGTATGAACATATAG
CAAAAACTTTAGATATACCAATGATTCTTATAGATATACCATATAATACTGAAGACACTATTTCACAGGATAGAATTAAA
TATATAAGAGCTCAGTTTGATAATGCAATAAAACAGTTAGAAGAAATAACAGGGAAAAAATGGGATGAAGATAAATTTGA
AGAAGTTATGAAGATATCTCAAGAGAGTGCAAAACAATGGCTAAGAGCAGCATCCTATGCAAAATATAAACCTTCACCAT
TTAGTGGATTCGATTTATTTAACCATATGGCTGTAGCAGTTTGTGCAAGGGGTACACAAGAAGCTGCAGATGCATTTAAG
ATGTTAGCAGATGAATATGAGGAAAATGTAAAGACTGGAAAGTCCACTTATAGAGGAGAAGAAAAACAACGTATATTATT
TGAAGGAATTGCCTGTTGGCCATATTTGAGACATAAATTAACTAAACTTAGTGAATATGGCATGAATGTAACTGCAACTG
TGTATGCTGAAGCTTTTGGTGTTATATATGAAAATACAGATGAATTAATGGCTGCTTACAATAAAGTTCCTAATTCAATT
AGTTTTGAAAATGCGTTAAAGATGAGATTAAATGCTGTTACAAGCACTAATACAGAAGGTGCTGTTATTCATATAAACAG
AAGCTGTAAACTGTGGAGTGGATTTTTATATGAGCTAGGAAGAAGATTAGAAAAGGAGACCGGAATTCCTGTAGTATCAT
TTGATGGGGATCAAGCAGACCCAAGAAATTTCTCAGAAGCACAATATGATACTAGAATTCAAGGACTTAATGAAGTAATG
GTTGCTAAAAAGGAGGCTGAATAA

Upstream 100 bases:

>100_bases
GGAAAAGACATTTATATATCTGAAAATTCCCAATTGAATGGAGCATTGGGAGCAAGTCTATACGCTTATGAAAGTTTTCA
AAAAGAAAGGAGCTAAAAAC

Downstream 100 bases:

>100_bases
GATGTCAAATTCAGATAAATTCTTTAATGACTTTAAGGATGTTATAGACCATCCTAAAAAATATATAATGAAGCATATAG
AAAAAACTGGGCAAAAGGCT

Product: R-phenyllactate dehydratase medium subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSDRNKEVKEKKAKHYLREITAKHYKEALEAKERGEKVGWCASNFPQEIATTLGIKVVYPENHAAAVAARGNGQNMCEHA
EAMGFSNDVCGYARVNLAVMDIGHSEDQPIPMPDFVLCCNNICNQMIKWYEHIAKTLDIPMILIDIPYNTEDTISQDRIK
YIRAQFDNAIKQLEEITGKKWDEDKFEEVMKISQESAKQWLRAASYAKYKPSPFSGFDLFNHMAVAVCARGTQEAADAFK
MLADEYEENVKTGKSTYRGEEKQRILFEGIACWPYLRHKLTKLSEYGMNVTATVYAEAFGVIYENTDELMAAYNKVPNSI
SFENALKMRLNAVTSTNTEGAVIHINRSCKLWSGFLYELGRRLEKETGIPVVSFDGDQADPRNFSEAQYDTRIQGLNEVM
VAKKEAE

Sequences:

>Translated_407_residues
MSDRNKEVKEKKAKHYLREITAKHYKEALEAKERGEKVGWCASNFPQEIATTLGIKVVYPENHAAAVAARGNGQNMCEHA
EAMGFSNDVCGYARVNLAVMDIGHSEDQPIPMPDFVLCCNNICNQMIKWYEHIAKTLDIPMILIDIPYNTEDTISQDRIK
YIRAQFDNAIKQLEEITGKKWDEDKFEEVMKISQESAKQWLRAASYAKYKPSPFSGFDLFNHMAVAVCARGTQEAADAFK
MLADEYEENVKTGKSTYRGEEKQRILFEGIACWPYLRHKLTKLSEYGMNVTATVYAEAFGVIYENTDELMAAYNKVPNSI
SFENALKMRLNAVTSTNTEGAVIHINRSCKLWSGFLYELGRRLEKETGIPVVSFDGDQADPRNFSEAQYDTRIQGLNEVM
VAKKEAE
>Mature_406_residues
SDRNKEVKEKKAKHYLREITAKHYKEALEAKERGEKVGWCASNFPQEIATTLGIKVVYPENHAAAVAARGNGQNMCEHAE
AMGFSNDVCGYARVNLAVMDIGHSEDQPIPMPDFVLCCNNICNQMIKWYEHIAKTLDIPMILIDIPYNTEDTISQDRIKY
IRAQFDNAIKQLEEITGKKWDEDKFEEVMKISQESAKQWLRAASYAKYKPSPFSGFDLFNHMAVAVCARGTQEAADAFKM
LADEYEENVKTGKSTYRGEEKQRILFEGIACWPYLRHKLTKLSEYGMNVTATVYAEAFGVIYENTDELMAAYNKVPNSIS
FENALKMRLNAVTSTNTEGAVIHINRSCKLWSGFLYELGRRLEKETGIPVVSFDGDQADPRNFSEAQYDTRIQGLNEVMV
AKKEAE

Specific function: Catalyzes the reversible dehydration of 2- hydroxyglutaryl-CoA to glutaconyl-CoA. This is a key step in the fermentation of glutamate. It is activated by hgdC [H]

COG id: COG1775

COG function: function code E; Benzoyl-CoA reductase/2-hydroxyglutaryl-CoA dehydratase subunit, BcrC/BadD/HgdB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010327 [H]

Pfam domain/function: PF06050 HGD-D [H]

EC number: NA

Molecular weight: Translated: 46223; Mature: 46092

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRNKEVKEKKAKHYLREITAKHYKEALEAKERGEKVGWCASNFPQEIATTLGIKVVYP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCEEEEEC
ENHAAAVAARGNGQNMCEHAEAMGFSNDVCGYARVNLAVMDIGHSEDQPIPMPDFVLCCN
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHEEEEEEEECCCCCCCCCCCHHHHHHHH
NICNQMIKWYEHIAKTLDIPMILIDIPYNTEDTISQDRIKYIRAQFDNAIKQLEEITGKK
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
WDEDKFEEVMKISQESAKQWLRAASYAKYKPSPFSGFDLFNHMAVAVCARGTQEAADAFK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
MLADEYEENVKTGKSTYRGEEKQRILFEGIACWPYLRHKLTKLSEYGMNVTATVYAEAFG
HHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
VIYENTDELMAAYNKVPNSISFENALKMRLNAVTSTNTEGAVIHINRSCKLWSGFLYELG
HHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
RRLEKETGIPVVSFDGDQADPRNFSEAQYDTRIQGLNEVMVAKKEAE
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDRNKEVKEKKAKHYLREITAKHYKEALEAKERGEKVGWCASNFPQEIATTLGIKVVYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCEEEEEC
ENHAAAVAARGNGQNMCEHAEAMGFSNDVCGYARVNLAVMDIGHSEDQPIPMPDFVLCCN
CCCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHEEEEEEEECCCCCCCCCCCHHHHHHHH
NICNQMIKWYEHIAKTLDIPMILIDIPYNTEDTISQDRIKYIRAQFDNAIKQLEEITGKK
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
WDEDKFEEVMKISQESAKQWLRAASYAKYKPSPFSGFDLFNHMAVAVCARGTQEAADAFK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
MLADEYEENVKTGKSTYRGEEKQRILFEGIACWPYLRHKLTKLSEYGMNVTATVYAEAFG
HHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
VIYENTDELMAAYNKVPNSISFENALKMRLNAVTSTNTEGAVIHINRSCKLWSGFLYELG
HHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
RRLEKETGIPVVSFDGDQADPRNFSEAQYDTRIQGLNEVMVAKKEAE
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2659350; 3691501; 8365476; 11106419 [H]