Click here to switch to the map view.

The map label for this gene is tig

Identifier: 148381181

GI number: 148381181

Start: 3442238

End: 3443530

Strand: Reverse

Name: tig

Synonym: CBO3232

Alternate gene names: 148381181

Gene position: 3443530-3442238 (Counterclockwise)

Preceding gene: 148381182

Following gene: 148381180

Centisome position: 88.59

GC content: 28.85

Gene sequence:

>1293_bases
ATGAACGTTAAAGTGGAAAATATAGAAAAGAATGTTGTAAAATTAGAAATAACAGTTGATAGTGAAAAATTCAATGAAGC
TGTTAAAAAATCATTTAAAAAGAATGCTAAAAGATTCAATGTACCAGGATTTAGAAAAGGTAAAGCACCTTTAAATATAA
TAAAAAAATACTATGGGGAAGGTGTTTTATTTGAAGATGCTATAAATTTCTGTTGTGAAGATACATATCCTAAAGCCATA
GAAGAAAATAACATAAAACCAGTAGATTATCCTCAAATAGATGTAGTTCAAATAGGAGAAGGAAAAGATTTCATATATAC
AGCAGAAGTTACAACTGTTCCTGAGGTTAAACTAGGAGAATATAAGGGCGTAGAAGTTAAAAAAGTTTCATATGAAGTAG
AAGATGAAGCTGTAGAAAATGAATTAAAATCCATGCAAGAAAAAAATGCAAGAGTATCTCTAAAAGAAGAAGGGGAAATT
GAAAAAGGAAACATAGCAATAATTGATTTTAAAGGATATGTAGATGGAAAAGCTTTTGAAGGTGGAGAGGCTAAGGATTA
TGAAATAGAAATAGGTTCAGGCACTTTCATAGGAGATTTTGAAGATCAATTAGTAGGTCTTAAAAAAGATGAATCAAAAG
AAGTAAATGTAAGTTTCCCAGAAGAATATGGTAGAGAAGATTTAAATGGAAAACCAGCTACTTTTGAAGTAACAATTAAA
GATATAAAGGTTAAAGAGCTTCCAGCATTAGATGATGAATTTGCAAAAGAAGTTTCAGAATTTGATACATTAGAGGAATT
AAAATCAGATATAAAGGATAGAATGAAAAAAGAACTATCTGAAAAAGCTAAAGCAGAATATGAAGAAGCAGTAGTTGAAG
CAGTTGGGGCAAATGCTGAAATAGAAATACCAAAAGTTATGATTGAAAAAGAAATAGAAAATATGGTAAGAGATTTAGAA
ATGAGATTAAAATACCAAGGCTTAGACCTAAAATCATATTATGAATTTACAAATAGTTCAGAAGAAAAAGTAAAAGAATA
TATGAGAGAAACAGCTGAAAAAAGAGTTAAAACAGATTTAATAATGCAAGAAATTGCTAAAGTAGAAGATATAAAAGCTA
CAGAAGAGGAATTAAAAGAAAAGGCTATGGAAGTAGCTAAACAATATGGTCAAAAAGATGTAGAAAAAACTGCTGAGTTA
ATAGCAAATGCTCAAAAAGCATACTTAGAAATTGATATAGTTAATGGAAAAGTTTTAGACCTATTGGTAGAGAGTAGCAA
AGAAATAGCTTAG

Upstream 100 bases:

>100_bases
TACTTTATGTTAGCGTAATGTATGTTAATAGTTCTAAATATATGATATAATGATATAGTTAAAATGAGCATATAACAAGG
AATTGGGAGGAAGAAGTATA

Downstream 100 bases:

>100_bases
TTCATTTATAATAATAGATAATGATATAGTTGCCAGGATATATTTTCTGGCAACTAAGTTTTAATAATTATAAATATCAC
ATAGGTATCATTAGTATCTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGEGVLFEDAINFCCEDTYPKAI
EENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPEVKLGEYKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEI
EKGNIAIIDFKGYVDGKAFEGGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAEIEIPKVMIEKEIENMVRDLE
MRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDLIMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAEL
IANAQKAYLEIDIVNGKVLDLLVESSKEIA

Sequences:

>Translated_430_residues
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGEGVLFEDAINFCCEDTYPKAI
EENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPEVKLGEYKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEI
EKGNIAIIDFKGYVDGKAFEGGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAEIEIPKVMIEKEIENMVRDLE
MRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDLIMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAEL
IANAQKAYLEIDIVNGKVLDLLVESSKEIA
>Mature_430_residues
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGEGVLFEDAINFCCEDTYPKAI
EENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPEVKLGEYKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEI
EKGNIAIIDFKGYVDGKAFEGGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAEIEIPKVMIEKEIENMVRDLE
MRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDLIMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAEL
IANAQKAYLEIDIVNGKVLDLLVESSKEIA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=417, Percent_Identity=31.89448441247, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CLOB1 (A7FYI3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385556.1
- ProteinModelPortal:   A7FYI3
- STRING:   A7FYI3
- GeneID:   5397214
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3269
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   CBOT441770:CLB_3269-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48925; Mature: 48925

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGE
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GVLFEDAINFCCEDTYPKAIEENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPEVKLGE
CEEHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCC
YKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEIEKGNIAIIDFKGYVDGKAFE
CCCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCC
GGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
CCCCCCEEEEECCCCEECCCHHHHCCCCCCCCCCEEEECCHHHCCCCCCCCCCEEEEEEE
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IEIPKVMIEKEIENMVRDLEMRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDL
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAELIANAQKAYLEIDIVNGKVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCHHHH
LLVESSKEIA
HHHHCCCCCC
>Mature Secondary Structure
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGE
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GVLFEDAINFCCEDTYPKAIEENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPEVKLGE
CEEHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCC
YKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEIEKGNIAIIDFKGYVDGKAFE
CCCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCC
GGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
CCCCCCEEEEECCCCEECCCHHHHCCCCCCCCCCEEEECCHHHCCCCCCCCCCEEEEEEE
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IEIPKVMIEKEIENMVRDLEMRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDL
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAELIANAQKAYLEIDIVNGKVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCHHHH
LLVESSKEIA
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA