BacMap

Definition	Clostridium botulinum A str. ATCC 3502, complete genome.
Accession	NC_009495
Length	3,886,916

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The map label for this gene is crt [H]

Identifier: 148381150

GI number: 148381150

Start: 3410980

End: 3411762

Strand: Reverse

Name: crt [H]

Synonym: CBO3202

Alternate gene names: 148381150

Gene position: 3411762-3410980 (Counterclockwise)

Preceding gene: 148381151

Following gene: 148381149

Centisome position: 87.78

GC content: 32.31

Gene sequence:

>783_bases
GTGGAAAGTAAAAATATCTTATTAGATAAAAAAAATCATATTGCTATTTTAAGTTTAAATAGAGAATCCGTACTTAATGC
TTTAAACACTCAGGTATTAAAAGAGATTAATATAATTTTAGATGATATAGAAAATGATGAAGATATTTATGTAGTTATAG
TAACTGGAAAAGGAAAGGCCTTTATAGCTGGAGCTGATATCTCAGAAATGAAGGATAAAACTCCTAAGGAAGGAAGAATA
TTTGGAGAATTAGGACAGAACATATGTAGAAAGATAGAAACCATGGAAAAGCCGACTATAGCAGCCGTAAATGGATATGC
TTTAGGTGGTGGATGCGAATTAGCAATGAGTTGCGATATAAGAATAGCTTCAGAAAAAGCTAAATTCGGTCAACCAGAAG
TAGGTTTAGGAATAACTCCAGGATTCGGAGGAACACAAAGGCTTTCCCGGTTAGTAGGGTTAAGTAAGGCAAAGGAATTG
ATACTTACTACAGATATTATAAAATCCCAGGAAGCGTTAAATATAGGGCTCGTTAATAAAGTAGTACCAGTAGAAAGCTT
ATTAGAAGAGGCAATAATGTTGGCAGAAAAAATAGTATCTAAACCTCAATCCGCTGTAAGATATGCTAAATCAGCTATAA
ATAGGGGGTATGAAACAGATATTGAAACAGGAATGATAATTGAAAAGGACTTATTCGGTTTATGTTTTGCAACAGAAGAC
CAAAAAGAAGGAATGGGAGCTTTTTTAGAAAAAAGAAAACCAAATTTTATTAATAAATATTAA

Upstream 100 bases:

>100_bases
CTTATTATTGTTAAAATAATAGCTATTAATTTTTGCGCAAAAAACAGATTACATATACTGGCATGAAAGTTGCTTCTTAT
AAAAGTAAGGGGGTGGTTAA

Downstream 100 bases:

>100_bases
ATAAATATTAAATTTTAAGGGGGTATAAAAATGAAAGTATTTATTTTAGGTGGCGGAACAATGGGAGCAGGCATCGTTGA
AGTTTTTGCTAAAGCCGGCC

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MESKNILLDKKNHIAILSLNRESVLNALNTQVLKEINIILDDIENDEDIYVVIVTGKGKAFIAGADISEMKDKTPKEGRI
FGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDIRIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKEL
ILTTDIIKSQEALNIGLVNKVVPVESLLEEAIMLAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
QKEGMGAFLEKRKPNFINKY

Sequences:

>Translated_260_residues
MESKNILLDKKNHIAILSLNRESVLNALNTQVLKEINIILDDIENDEDIYVVIVTGKGKAFIAGADISEMKDKTPKEGRI
FGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDIRIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKEL
ILTTDIIKSQEALNIGLVNKVVPVESLLEEAIMLAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
QKEGMGAFLEKRKPNFINKY
>Mature_260_residues
MESKNILLDKKNHIAILSLNRESVLNALNTQVLKEINIILDDIENDEDIYVVIVTGKGKAFIAGADISEMKDKTPKEGRI
FGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDIRIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKEL
ILTTDIIKSQEALNIGLVNKVVPVESLLEEAIMLAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
QKEGMGAFLEKRKPNFINKY

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=48.1781376518219, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=36.8627450980392, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=34.7107438016529, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=36.7647058823529, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI20127408, Length=215, Percent_Identity=35.8139534883721, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=32.8, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=29.5652173913043, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=29.5652173913043, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI70995211, Length=223, Percent_Identity=35.4260089686099, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI37594469, Length=250, Percent_Identity=30.4, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=250, Percent_Identity=30.4, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=29.5, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI62530384, Length=257, Percent_Identity=25.2918287937743, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI261878539, Length=108, Percent_Identity=42.5925925925926, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=202, Percent_Identity=28.2178217821782, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI221136753, Length=202, Percent_Identity=28.2178217821782, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=28.2178217821782, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI116235487, Length=189, Percent_Identity=27.5132275132275, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI295842266, Length=255, Percent_Identity=23.1372549019608, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=25.7009345794392, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=25.7009345794392, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=25.7009345794392, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI4757966, Length=202, Percent_Identity=25.2475247524752, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=202, Percent_Identity=25.2475247524752, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25453481, Length=202, Percent_Identity=25.2475247524752, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538573, Length=202, Percent_Identity=25.2475247524752, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=202, Percent_Identity=24.7524752475248, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI49355787, Length=202, Percent_Identity=24.7524752475248, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=41.0358565737052, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=35.2, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=40, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=32.9365079365079, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=31.5175097276265, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=33.2, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=37.0165745856354, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=43.4782608695652, Blast_Score=211, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=43.6, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=39.3939393939394, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=30.4347826086957, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=37.0165745856354, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=29.2134831460674, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=36.6666666666667, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=36.6666666666667, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=36.6666666666667, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=30.9644670050761, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=254, Percent_Identity=30.3149606299213, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=186, Percent_Identity=32.258064516129, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=186, Percent_Identity=32.258064516129, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=193, Percent_Identity=31.6062176165803, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=24.7933884297521, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=197, Percent_Identity=27.4111675126904, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=219, Percent_Identity=26.4840182648402, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=48.582995951417, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=48.582995951417, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=39.4422310756972, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=30.7116104868914, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24650670, Length=216, Percent_Identity=31.4814814814815, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=25.9842519685039, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19920382, Length=253, Percent_Identity=31.6205533596838, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19921000, Length=176, Percent_Identity=33.5227272727273, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24583077, Length=176, Percent_Identity=33.5227272727273, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583079, Length=176, Percent_Identity=33.5227272727273, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654903, Length=224, Percent_Identity=26.3392857142857, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921018, Length=251, Percent_Identity=27.8884462151394, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583165, Length=175, Percent_Identity=29.7142857142857, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550169, Length=188, Percent_Identity=26.063829787234, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28450; Mature: 28450

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKNILLDKKNHIAILSLNRESVLNALNTQVLKEINIILDDIENDEDIYVVIVTGKGKA
CCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
FIAGADISEMKDKTPKEGRIFGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDI
EEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCEEEEEEECE
RIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKELILTTDIIKSQEALNIGLVNK
EEECCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHH
VVPVESLLEEAIMLAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
HCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHEEEEECCC
QKEGMGAFLEKRKPNFINKY
HHCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MESKNILLDKKNHIAILSLNRESVLNALNTQVLKEINIILDDIENDEDIYVVIVTGKGKA
CCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
FIAGADISEMKDKTPKEGRIFGELGQNICRKIETMEKPTIAAVNGYALGGGCELAMSCDI
EEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCEEEEEEECE
RIASEKAKFGQPEVGLGITPGFGGTQRLSRLVGLSKAKELILTTDIIKSQEALNIGLVNK
EEECCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCHHHHHH
VVPVESLLEEAIMLAEKIVSKPQSAVRYAKSAINRGYETDIETGMIIEKDLFGLCFATED
HCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHEEEEECCC
QKEGMGAFLEKRKPNFINKY
HHCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]