Definition Clostridium botulinum A str. ATCC 3502, complete genome.
Accession NC_009495
Length 3,886,916

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The map label for this gene is thrS

Identifier: 148381087

GI number: 148381087

Start: 3338035

End: 3339942

Strand: Reverse

Name: thrS

Synonym: CBO3138

Alternate gene names: 148381087

Gene position: 3339942-3338035 (Counterclockwise)

Preceding gene: 148381088

Following gene: 148381086

Centisome position: 85.93

GC content: 30.82

Gene sequence:

>1908_bases
ATGATAAAAATCACATTAAAAGATGGCAAAGTAATGGAATTTGAAGAAGGAATAAAAATATCTGATATTGCTATGAAAAT
TAGTCCAGCTCTTTATAAAAAAGCTTTAGCAGCTAAAATAGATGGAGAAACTGTGGACTTAATGACTGAGCTACACAAAG
ATAGTAGCTTAGAAATTTTAACATTTGAAGATGAAATGGGTAAATGGGCTTTAAGACATACTGGTTCACATATGCTAGCT
CAAGCAGTTAAAAGATTATACCCAGAAGTAAAATTAGCTATAGGACCTGCTATAGACACTGGCTTTTATTATGATTTTGA
AGCAGATTTTACTTTTACACCAGAAATGTTAGAAAAAATAGAAGCAGAAATTAAAAAAATAATAAAAGAAAATCATAAGT
TAGAAAGATTTGAATTACCAAGAGAAGAAGCAATAAAGCTTATGAAAGAAAAGAATGAAGATTATAAGGTTGAGCTTATA
GAAGATTTACCAGAAGGAGAAGTTATATCTTTCTATAAACAAGGAGATTTTACAGATTTATGTGCAGGACCTCATGTACC
ATCTACAGGAAAGGTTAAATCAGTAAAATTGTTATCTCTAGCTGGCGCTTATTGGAGAGGCGATGAAAATAATAAAATGC
TTCAAAGGATATATGGTACAGCCTTTACTAAAAAAAGTGAGCTAGACGAATATTTAAATATGATTGAAGAAGCTAAAAAA
AGAGATCATAGAAAATTGGGTAAAGAATTAGATTTATTTAGTATACATGAAGAGGGTCCAGGATTCCCATTTTTCCATCC
AAAGGGAATGATTGTAAGAAACATTCTTGAAAGTTTTTGGAGGGAAGAGCACACAAAAGCAGGATATCAAGAAATAAGGA
CACCATTAATATTAAATGAAGCTTTATGGCATCAATCAGGTCACTGGGATCACTACAAAGAAAATATGTATTTTACTAAT
ATAGATGATGATGATTATGCAATAAAACCTATGAATTGTCCAGGTGGAATACTAGTCTATAAGAATTCTATGCATTCTTA
TAGAGATCTTCCATTAAGACTTTCAGAATTAGGAATAGTTCATAGACATGAATTATCAGGAGCATTACATGGACTTATGA
GAGTAAGATGTTTTACTCAAGATGATGCTCATTTATATATGACAAAAGAACAGATAAAAGAAGAAGTAGTAGGAATAATT
AAATTAATAGATAAATTCTATAAACTATTTGGATTTGAATATTTTGTAGAGCTTTCAACAAGACCAGAGGATTCTATGGG
AAGTGATGAAGATTGGGAAATAGCAACTAATGGTTTAAGAGAAGCTTTAGATTCTATAGGAAAAGAATATAGAGTAAATG
AAGGTGACGGAGCTTTCTATGGTCCAAAGATAGATTTCCATTTAAAGGACTGTATAGGCAGAACATGGCAATGTGGAACT
ATTCAACTTGATTTCCAAATGCCAGAAAGATTTGATTTATCATATATAGGTGCAGATGGTGAAAAGCATAGACCTGTTAT
GGTTCATAGAACTATATATGGAAGTGTTGAAAGATTTATAGGTATATTAATAGAGCAATACGCAGGTGCATTCCCAACAT
GGCTTGCTCCAGTACAAGTTAAATTAATGAATATAACTGATTCTCAATATGATTATTTAAAGAAAGTTGAAGAAGCTCTT
AAAGAAAATAATATAAGGGTAGAAATTGATACAAGAAATGAAAAAATAGGGTATAAGATAAGAGAAGCTCAACTTCAAAA
AGTACCGTATATGCTAATACTTGGAGATAAAGAAGTGGAAGCAGGAAAAGTTGCTGTAAGAAGTAGAAAAGATGGAGATT
TAGGGGCTATATCCTTAGAAGAATTTATAGAAAAAATAAAGAATGAAATTAAGAACAAAACTAACTAA

Upstream 100 bases:

>100_bases
CACTGCTATTAAATTGGGTGGAACCACGAAAATACACTCGTCCCATGTGTTGGGACGGGTTTTTTTATTTGTAAATTTTT
ATTTGAAGGAGGACAATTTT

Downstream 100 bases:

>100_bases
AAATAAATTAATTTATAATAATAAAGGAATTTAAATTTTTTATAGAAAAATATTGACTTGCTAATACATCCATAGTATAA
TACTATTTGTTGATTAACTT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEILTFEDEMGKWALRHTGSHMLA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKIEAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGDENNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDDDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDSQYDYLKKVEEAL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN

Sequences:

>Translated_635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEILTFEDEMGKWALRHTGSHMLA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKIEAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGDENNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDDDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDSQYDYLKKVEEAL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN
>Mature_635_residues
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEILTFEDEMGKWALRHTGSHMLA
QAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKIEAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELI
EDLPEGEVISFYKQGDFTDLCAGPHVPSTGKVKSVKLLSLAGAYWRGDENNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNEALWHQSGHWDHYKENMYFTN
IDDDDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIVHRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGII
KLIDKFYKLFGFEYFVELSTRPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQVKLMNITDSQYDYLKKVEEAL
KENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVEAGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=631, Percent_Identity=41.6798732171157, Blast_Score=510, Evalue=1e-144,
Organism=Homo sapiens, GI38570111, Length=638, Percent_Identity=40.9090909090909, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI20070344, Length=652, Percent_Identity=37.7300613496933, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=48.5849056603774, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=202, Percent_Identity=25.2475247524752, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=630, Percent_Identity=40.4761904761905, Blast_Score=514, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71984192, Length=630, Percent_Identity=40.4761904761905, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6322112, Length=651, Percent_Identity=38.4024577572965, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=39.0428211586902, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=41.1111111111111, Blast_Score=513, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24583842, Length=629, Percent_Identity=40.8585055643879, Blast_Score=513, Evalue=1e-145,
Organism=Drosophila melanogaster, GI28574141, Length=629, Percent_Identity=40.8585055643879, Blast_Score=513, Evalue=1e-145,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_CLOB1 (A7FY87)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385460.1
- ProteinModelPortal:   A7FY87
- SMR:   A7FY87
- STRING:   A7FY87
- GeneID:   5396619
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3168
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   FHPKGMV
- ProtClustDB:   PRK00413
- BioCyc:   CBOT441770:CLB_3168-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73394; Mature: 73394

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEIL
CEEEEECCCCEEEHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
TFEDEMGKWALRHTGSHMLAQAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKI
EECCCCCCHHHHHCCHHHHHHHHHHHCCCEEEEECCEECCCEEEEECCCCEECHHHHHHH
EAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELIEDLPEGEVISFYKQGDFTDL
HHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCCCEEEHHHCCCCCCEEEEEECCCCCHH
CAGPHVPSTGKVKSVKLLSLAGAYWRGDENNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
CCCCCCCCCCCEEEEEHEEHHCHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
ALWHQSGHWDHYKENMYFTNIDDDDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIV
HHHCCCCCCCCHHCCEEEECCCCCCCEEEECCCCCCEEEEECCCCCHHCCCCCHHHHCCH
HRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGIIKLIDKFYKLFGFEYFVELST
HHHHHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
RPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEEEEHHHHCCCCEECCE
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQV
EEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KLMNITDSQYDYLKKVEEALKENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVE
EEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCC
AGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN
CCCEEEECCCCCCCCEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKITLKDGKVMEFEEGIKISDIAMKISPALYKKALAAKIDGETVDLMTELHKDSSLEIL
CEEEEECCCCEEEHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEE
TFEDEMGKWALRHTGSHMLAQAVKRLYPEVKLAIGPAIDTGFYYDFEADFTFTPEMLEKI
EECCCCCCHHHHHCCHHHHHHHHHHHCCCEEEEECCEECCCEEEEECCCCEECHHHHHHH
EAEIKKIIKENHKLERFELPREEAIKLMKEKNEDYKVELIEDLPEGEVISFYKQGDFTDL
HHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCCCEEEHHHCCCCCCEEEEEECCCCCHH
CAGPHVPSTGKVKSVKLLSLAGAYWRGDENNKMLQRIYGTAFTKKSELDEYLNMIEEAKK
CCCCCCCCCCCEEEEEHEEHHCHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFSIHEEGPGFPFFHPKGMIVRNILESFWREEHTKAGYQEIRTPLILNE
HHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
ALWHQSGHWDHYKENMYFTNIDDDDYAIKPMNCPGGILVYKNSMHSYRDLPLRLSELGIV
HHHCCCCCCCCHHCCEEEECCCCCCCEEEECCCCCCEEEEECCCCCHHCCCCCHHHHCCH
HRHELSGALHGLMRVRCFTQDDAHLYMTKEQIKEEVVGIIKLIDKFYKLFGFEYFVELST
HHHHHHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
RPEDSMGSDEDWEIATNGLREALDSIGKEYRVNEGDGAFYGPKIDFHLKDCIGRTWQCGT
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEEEEHHHHCCCCEECCE
IQLDFQMPERFDLSYIGADGEKHRPVMVHRTIYGSVERFIGILIEQYAGAFPTWLAPVQV
EEEEEECCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
KLMNITDSQYDYLKKVEEALKENNIRVEIDTRNEKIGYKIREAQLQKVPYMLILGDKEVE
EEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCCCC
AGKVAVRSRKDGDLGAISLEEFIEKIKNEIKNKTN
CCCEEEECCCCCCCCEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA