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The map label for this gene is ydeM [C]

Identifier: 148381015

GI number: 148381015

Start: 3252405

End: 3253772

Strand: Reverse

Name: ydeM [C]

Synonym: CBO3064

Alternate gene names: 148381015

Gene position: 3253772-3252405 (Counterclockwise)

Preceding gene: 148381016

Following gene: 148381014

Centisome position: 83.71

GC content: 28.73

Gene sequence:

>1368_bases
TTGGCTGATATTCATAAGTTCACCCAAATGGGACAAAAATATGTTATAGACGTAAATTCAGGAGCAGTGCATATAGTAGA
TGATATAGTATATGATTTATTAGATGATGAAGGTTTAAAAACTAAAGAAGATCTTATATATGAATATAAAGAAAAATACA
GCAAAGAGGAGATAGAAGAGGTTTATGAAGAAATAGAAAGTCTTATAAAAGAGGACATGCTATATTCTAAAGATAAATAT
GAAAATATAGCTTATGCCCATGAAAAAAGCGAGAATTTTATAAAGGCTTTATGCTTAAATATAGCCCATGATTGTAATTT
AAGATGTAAATATTGTTTTGCAGATGAAGGAGAATATAAAGGTAAAAGAGAATTAATGTCTCCAGGGGTTGGTAAAAAAG
CTATAGATTTTGTTATAGAGAAATCTGGACCAAGAAAAAATATAGAGGTAGATTTATTTGGAGGAGAACCATTAATGGCT
TTTTCTACTATAAAGGAAATAGTAGAATATGCTAAAGAACAAGAGGAAAAACATAATAAGACTATAAGATTTACTATGAC
TACCAATGGTACTTTATTAAATCAGGAAATAATGGAATACCTGGATAAAAATATGGGAAATATAGTATTAAGTATAGATG
GAAGAAAAGAAATAAATGACAATGTTAGAGTTAGAGTAGATGGTAGTGGTTCTTATGACTCTATATTACCTAAAATTAAA
AAGATGGTAGAGATGAGGGATAGAACAAAACAATATTATGCTAGAGGTACATTTACAAGAGAAAATTTAGATTTTTTTGA
AGACATAATGCATATGGCTAATAATGACTTTGATGAAATATCTATAGAACCAGTAGTTCTTCCAGAAGATCACCCTTTAT
CTTTAAGAGAAGAAGATTTACCAAAAATATTTGAACAATATGATAAATTAATAGAAGAAATGATAAAAAGACATAAAGAA
GGTAAAGAATTTAAGTTTTATCACTTTAACATAGATCTTCAAGGTGGACCTTGCGTTTATAAAAGAATATCTGGTTGTGG
AGCAGGTCATGAATATGTAGCTATATCTCCATCAGGAGATGTTTATCCATGTCATCAATTTGTAGGAAATGAAGACTTTA
AAATGGGAAACATAATGAAGGACTTTGAGGTAAAGGAAGATACAGCAAAGGAATTTAAAAAAGCTCATATATACAATAAA
CCGGAATGTAAAGAATGTTGGGCAAGATTTTACTGTAGCGGAGGATGCCAAGCAAACAATTATAATTTTAATAAAGATAT
GAATATACCTTATAGTTTAGGATGTACTATGCAAAAAAAGAGAATAGAGTGTGCAATAACTTTAAAATCTAATACAATGA
ATGATTAA

Upstream 100 bases:

>100_bases
GTATAATATAAATGTTTTAATAAATAATAAGTCCTATATTTTGAAAATATTTGCAGAATTATAAAAGTAAAGTATATAGG
TTAATAAGGAGGAAATTTAA

Downstream 100 bases:

>100_bases
TAAAAGAAAATTAGTACATAATTTATTATAAAGCAAACATAATAAATTATGTTCTTATTCTTATAATAAACTATATATAA
AAGGGGGATAAGTATGAGTT

Product: radical SAM domain protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MADIHKFTQMGQKYVIDVNSGAVHIVDDIVYDLLDDEGLKTKEDLIYEYKEKYSKEEIEEVYEEIESLIKEDMLYSKDKY
ENIAYAHEKSENFIKALCLNIAHDCNLRCKYCFADEGEYKGKRELMSPGVGKKAIDFVIEKSGPRKNIEVDLFGGEPLMA
FSTIKEIVEYAKEQEEKHNKTIRFTMTTNGTLLNQEIMEYLDKNMGNIVLSIDGRKEINDNVRVRVDGSGSYDSILPKIK
KMVEMRDRTKQYYARGTFTRENLDFFEDIMHMANNDFDEISIEPVVLPEDHPLSLREEDLPKIFEQYDKLIEEMIKRHKE
GKEFKFYHFNIDLQGGPCVYKRISGCGAGHEYVAISPSGDVYPCHQFVGNEDFKMGNIMKDFEVKEDTAKEFKKAHIYNK
PECKECWARFYCSGGCQANNYNFNKDMNIPYSLGCTMQKKRIECAITLKSNTMND

Sequences:

>Translated_455_residues
MADIHKFTQMGQKYVIDVNSGAVHIVDDIVYDLLDDEGLKTKEDLIYEYKEKYSKEEIEEVYEEIESLIKEDMLYSKDKY
ENIAYAHEKSENFIKALCLNIAHDCNLRCKYCFADEGEYKGKRELMSPGVGKKAIDFVIEKSGPRKNIEVDLFGGEPLMA
FSTIKEIVEYAKEQEEKHNKTIRFTMTTNGTLLNQEIMEYLDKNMGNIVLSIDGRKEINDNVRVRVDGSGSYDSILPKIK
KMVEMRDRTKQYYARGTFTRENLDFFEDIMHMANNDFDEISIEPVVLPEDHPLSLREEDLPKIFEQYDKLIEEMIKRHKE
GKEFKFYHFNIDLQGGPCVYKRISGCGAGHEYVAISPSGDVYPCHQFVGNEDFKMGNIMKDFEVKEDTAKEFKKAHIYNK
PECKECWARFYCSGGCQANNYNFNKDMNIPYSLGCTMQKKRIECAITLKSNTMND
>Mature_454_residues
ADIHKFTQMGQKYVIDVNSGAVHIVDDIVYDLLDDEGLKTKEDLIYEYKEKYSKEEIEEVYEEIESLIKEDMLYSKDKYE
NIAYAHEKSENFIKALCLNIAHDCNLRCKYCFADEGEYKGKRELMSPGVGKKAIDFVIEKSGPRKNIEVDLFGGEPLMAF
STIKEIVEYAKEQEEKHNKTIRFTMTTNGTLLNQEIMEYLDKNMGNIVLSIDGRKEINDNVRVRVDGSGSYDSILPKIKK
MVEMRDRTKQYYARGTFTRENLDFFEDIMHMANNDFDEISIEPVVLPEDHPLSLREEDLPKIFEQYDKLIEEMIKRHKEG
KEFKFYHFNIDLQGGPCVYKRISGCGAGHEYVAISPSGDVYPCHQFVGNEDFKMGNIMKDFEVKEDTAKEFKKAHIYNKP
ECKECWARFYCSGGCQANNYNFNKDMNIPYSLGCTMQKKRIECAITLKSNTMND

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52884; Mature: 52752

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIHKFTQMGQKYVIDVNSGAVHIVDDIVYDLLDDEGLKTKEDLIYEYKEKYSKEEIEE
CCCHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHH
VYEEIESLIKEDMLYSKDKYENIAYAHEKSENFIKALCLNIAHDCNLRCKYCFADEGEYK
HHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
GKRELMSPGVGKKAIDFVIEKSGPRKNIEVDLFGGEPLMAFSTIKEIVEYAKEQEEKHNK
CHHHHCCCCCCHHHHHHHEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
TIRFTMTTNGTLLNQEIMEYLDKNMGNIVLSIDGRKEINDNVRVRVDGSGSYDSILPKIK
EEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHH
KMVEMRDRTKQYYARGTFTRENLDFFEDIMHMANNDFDEISIEPVVLPEDHPLSLREEDL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECEEECCCCCCCCCCHHHH
PKIFEQYDKLIEEMIKRHKEGKEFKFYHFNIDLQGGPCVYKRISGCGAGHEYVAISPSGD
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCEEEECCCCC
VYPCHQFVGNEDFKMGNIMKDFEVKEDTAKEFKKAHIYNKPECKECWARFYCSGGCQANN
EEEHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
YNFNKDMNIPYSLGCTMQKKRIECAITLKSNTMND
CCCCCCCCCCEEECCCEECCEEEEEEEEECCCCCC
>Mature Secondary Structure 
ADIHKFTQMGQKYVIDVNSGAVHIVDDIVYDLLDDEGLKTKEDLIYEYKEKYSKEEIEE
CCHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHH
VYEEIESLIKEDMLYSKDKYENIAYAHEKSENFIKALCLNIAHDCNLRCKYCFADEGEYK
HHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
GKRELMSPGVGKKAIDFVIEKSGPRKNIEVDLFGGEPLMAFSTIKEIVEYAKEQEEKHNK
CHHHHCCCCCCHHHHHHHEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
TIRFTMTTNGTLLNQEIMEYLDKNMGNIVLSIDGRKEINDNVRVRVDGSGSYDSILPKIK
EEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHH
KMVEMRDRTKQYYARGTFTRENLDFFEDIMHMANNDFDEISIEPVVLPEDHPLSLREEDL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECEEECCCCCCCCCCHHHH
PKIFEQYDKLIEEMIKRHKEGKEFKFYHFNIDLQGGPCVYKRISGCGAGHEYVAISPSGD
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCEEEECCCCC
VYPCHQFVGNEDFKMGNIMKDFEVKEDTAKEFKKAHIYNKPECKECWARFYCSGGCQANN
EEEHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
YNFNKDMNIPYSLGCTMQKKRIECAITLKSNTMND
CCCCCCCCCCEEECCCEECCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA