| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is mrdA [H]
Identifier: 148380950
GI number: 148380950
Start: 3192273
End: 3195047
Strand: Reverse
Name: mrdA [H]
Synonym: CBO2999
Alternate gene names: 148380950
Gene position: 3195047-3192273 (Counterclockwise)
Preceding gene: 148380951
Following gene: 148380949
Centisome position: 82.2
GC content: 31.28
Gene sequence:
>2775_bases ATGAAGATAAACAAAGATAAAAAATTTACTAGATTTGGTGGTCTTATGTTGGTAATGGTATTTATAATTACCGCAATTAT ATCTAGACTTGTTTATTTGCAACTTATGAATTCACAGGAGTACAAAGAAAAAGCAAATAATAGATCCATAAGAGAAATTC CAGATCCTGCTCCAAGAGGGAATATAATAGATAGAAATGGAGTAGTATTAGCCACTAATAAGCAAAATTACATGCTTATA TATAATGAAACAGCAGAAAATAAAGAAAGTTTTTTTTCTACTATGGAAAAAGTATTTAGCATATTAGATCAATATAAAGA AAAACAAACGGATGATTTTGAACTTAAAATTAATCCTTATAGATTTGAATTTAAAGCTAGTACAGCTGAGGCAAAAAGAG CAGCAGAGTTAAGATTTAAAAAAGATAGAGGATTAGATGATGTAGTTACAAAAAAATTATTTTCAGGTAAAAACAAAAAA GAAAAAGAAGAATTAACAAAAGAAGATAAAGCTAAAATAGATGAGGAGTTATTAAAAATAACTCCAGAGGAAACCTTTAA ATATTTATTAGAACAATATAAAATAGATACAAAGAAATATTCTCTAGAACAGCAAAGAAGATTTATGATAGTAAAAGATG CAGCAAAAATGCAGAGCTTTTCAGGATATAAATCTGTTGATGTGGCAAGTAATATAAAGAAAGAAACAGCTTTTATATTT CTTCAGAAATTAAATGATTTACCAGGTATAGATGTAAGCACTCAACCCATAAGAGTTTATCCTTATAAGGAAGTAGGATC AGCAGTACTAGGCTATATATCTAAAATAAGTGGTGATAATGAGAAATATAAAGAAAAGGGATATGACCCAAGTTCTGATT ATATAGGTATAGATGGAATAGAGGGTGTTTTTGAAGAAAGACTTAAGGGATCTAAAGGTGGAAGAATTGTTAAACTTAAT AAAACAGGAAGGGTTATAGAAGAATTAGGAAGAAGAGAACCTTATCCAGGTCAAAATATACAACTTACTATAGATACGAA TATACAGAAGGCTGCAGAAGAGTCGCTGGATTCTGTAATGAAAGATCTTCAACAAAGAGGAGTACAAGGCGATGTTAATA CATCTAATGCTACAAGAGGTGCTGCAGTAGCATTAGATGTAAATACAGGAGAAATTTTAGCTTTGGCAAGTAGGCCAGGC TTTGATCCTAACTTATTTGCAACTCCAGGAGGATTAACCCCAGAACTATATAAACAATATTTTAACCCGGATTTAACTGA GTTTGGAAAACAATATATACTTAATAAAGGATTAATGGGCTATTATCCGGGAAATACTTTAGACGAGGTTTTAGAAAAGT TATTTCCTTTAGATAAAAGTATAAAAAATAATAAAACTATGAGGCAAGATTTATATGATATATATCCAAAGCCTTTTTAT AATTATGCTACTATGTCTGTAGTACCTCCAGGATCTACATTTAAAGCTATGACAGCTATTGCAGGATTAGAATCAGGGGT TATAACTCCTGGATATTCTATAAGCGATACGGGCGTTTTTGATGACGGAAAGAAGTTTGTAAAGAAATTCGCAGTAGGTG GCTATGGTTCCGTTGATTTATATAGAGGATTAGAGGTTTCTAGTAACCCTTATTTTATGACAGTAGGTAAATTATTAAGA GAGAGTTTTGGTGATGATATACTTGCAAAATACGCTTGGAAATTTGGGTTAGGTGTTCCACCAAATTCAGATGAAAAAGC TTCTACAGGAATAGAAATTCCTGAAAAGTTTGGACAAGTATTTAATACTTATACATTATCTAATGTTTATGCAACTCAAT ACTTGTGGCAAACTATGTCTACTTTAAAATCAGGAACAGATGCTAGGGGAAATAAATTTCCATCTATAAATTTATATGAT AGCGAAAAAGATTCAGACAAGGTTAAGGAATTAAAGAAACAAATTAAAAATTCTATACAGGACTCTATAAAAGAAGGAAC TAAAAAGTTTGATTCTAATAATTATATAAAAATGCTTACAGAGTTAGTTAATCAAGATCCTATCTATAAAGGTAAAAATG TAAGTAAAGATCAAATAAAAAGAATTGTAGATGTTATATACTATGTAACAATATCAGATGCTCACTTCCAGTTAGGTGTA GGTGCTAATATGTACAATGCTGCTATAGGACAAGGTATAAGTAACTTTACTCCATTACAATTGGCTAATTTTGTTAGTAC TATAGCTAATGGAGGAGATAGATATAAATTGCATTTGGTGAAAGAAATAAAAGATGCAGATGGTAATGTAGTAGAAACAG TTAAACCAGAGATAGTTGAAAAGACTAATGTAAGCAAAGAGAATTTAGCTGCTGTAAGGCAAGGAATGGCACGAGTTAAT GAGGGAACAGATGGTACTGCAGCAGCAGTGTTTAAGGGTTTCCCTATACAAACAGCAGGTAAAACAGGATCTTCTACTTT TAGTAATAGTCAAGATTATTATGGAAGAACATCTTATGGTGTTTATATAGGATTTGCTCCAGTTGATAATCCAAAAATAG CAGTATGTGTTATTGTATTTGATGGTGGACACGGAGGATCTGTTGCACCTGTAGCAAAGGCTATGTATGAAGCCTATTTT AAGAAGGAACTTAATAAAGACAAACCAGATGCAGCTAAAGCATCAAATTCAGGGCAAAAGGAAAATACTAAGAATTCAGA TGCTAGCGGAGATGATGCAAAGGACATAGAAAATAAAAATACAAATAGCAGATAA
Upstream 100 bases:
>100_bases GGGGTAATAACAATTATAGTTTATAAATTAGTATATAATTTATGTAGAAAAAAATATATGGATAAAAAGTGGGAATTCTA AAGGAATGGTGATTTAGTCA
Downstream 100 bases:
>100_bases ATAAATATAAACTATATCCACACTATAAATTATTTTATATAATAGATAGTGTGGATATTTTAAAATGGAAAATATTAAAT AAATAAAAGTATAATAAACA
Product: penicillin-binding protein
Products: NA
Alternate protein names: PBP-2 [H]
Number of amino acids: Translated: 924; Mature: 924
Protein sequence:
>924_residues MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRGNIIDRNGVVLATNKQNYMLI YNETAENKESFFSTMEKVFSILDQYKEKQTDDFELKINPYRFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKK EKEELTKEDKAKIDEELLKITPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF LQKLNDLPGIDVSTQPIRVYPYKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGIEGVFEERLKGSKGGRIVKLN KTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEESLDSVMKDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPG FDPNLFATPGGLTPELYKQYFNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDLYRGLEVSSNPYFMTVGKLLR ESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQVFNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYD SEKDSDKVKELKKQIKNSIQDSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVEKTNVSKENLAAVRQGMARVN EGTDGTAAAVFKGFPIQTAGKTGSSTFSNSQDYYGRTSYGVYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYF KKELNKDKPDAAKASNSGQKENTKNSDASGDDAKDIENKNTNSR
Sequences:
>Translated_924_residues MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRGNIIDRNGVVLATNKQNYMLI YNETAENKESFFSTMEKVFSILDQYKEKQTDDFELKINPYRFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKK EKEELTKEDKAKIDEELLKITPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF LQKLNDLPGIDVSTQPIRVYPYKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGIEGVFEERLKGSKGGRIVKLN KTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEESLDSVMKDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPG FDPNLFATPGGLTPELYKQYFNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDLYRGLEVSSNPYFMTVGKLLR ESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQVFNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYD SEKDSDKVKELKKQIKNSIQDSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVEKTNVSKENLAAVRQGMARVN EGTDGTAAAVFKGFPIQTAGKTGSSTFSNSQDYYGRTSYGVYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYF KKELNKDKPDAAKASNSGQKENTKNSDASGDDAKDIENKNTNSR >Mature_924_residues MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRGNIIDRNGVVLATNKQNYMLI YNETAENKESFFSTMEKVFSILDQYKEKQTDDFELKINPYRFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKK EKEELTKEDKAKIDEELLKITPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF LQKLNDLPGIDVSTQPIRVYPYKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGIEGVFEERLKGSKGGRIVKLN KTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEESLDSVMKDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPG FDPNLFATPGGLTPELYKQYFNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDLYRGLEVSSNPYFMTVGKLLR ESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQVFNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYD SEKDSDKVKELKKQIKNSIQDSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVEKTNVSKENLAAVRQGMARVN EGTDGTAAAVFKGFPIQTAGKTGSSTFSNSQDYYGRTSYGVYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYF KKELNKDKPDAAKASNSGQKENTKNSDASGDDAKDIENKNTNSR
Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]
COG id: COG0768
COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2
Gene ontology:
Cell location: Cell inner membrane [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786854, Length=392, Percent_Identity=27.0408163265306, Blast_Score=108, Evalue=1e-24, Organism=Escherichia coli, GI1786272, Length=171, Percent_Identity=32.7485380116959, Blast_Score=82, Evalue=2e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR005311 - InterPro: IPR001460 - InterPro: IPR017790 [H]
Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]
EC number: NA
Molecular weight: Translated: 103074; Mature: 103074
Theoretical pI: Translated: 9.29; Mature: 9.29
Prosite motif: PS00216 SUGAR_TRANSPORT_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRG CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC NIIDRNGVVLATNKQNYMLIYNETAENKESFFSTMEKVFSILDQYKEKQTDDFELKINPY CEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCE RFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKKEKEELTKEDKAKIDEELLKI EEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC TPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF CHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHH LQKLNDLPGIDVSTQPIRVYPYKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGI HHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEECCCCH EGVFEERLKGSKGGRIVKLNKTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEESLDSVM HHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHH KDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPGFDPNLFATPGGLTPELYKQY HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEECCCCCCHHHHHHH FNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCC NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDL CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH YRGLEVSSNPYFMTVGKLLRESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQV HCCCCCCCCCEEEHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH FNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYDSEKDSDKVKELKKQIKNSIQ HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH DSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCEEEEEC GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVE CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCEEECCCHHHHH KTNVSKENLAAVRQGMARVNEGTDGTAAAVFKGFPIQTAGKTGSSTFSNSQDYYGRTSYG HCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE VYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYFKKELNKDKPDAAKASNSGQK EEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC ENTKNSDASGDDAKDIENKNTNSR CCCCCCCCCCCCHHHCCCCCCCCC >Mature Secondary Structure MKINKDKKFTRFGGLMLVMVFIITAIISRLVYLQLMNSQEYKEKANNRSIREIPDPAPRG CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC NIIDRNGVVLATNKQNYMLIYNETAENKESFFSTMEKVFSILDQYKEKQTDDFELKINPY CEECCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCE RFEFKASTAEAKRAAELRFKKDRGLDDVVTKKLFSGKNKKEKEELTKEDKAKIDEELLKI EEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC TPEETFKYLLEQYKIDTKKYSLEQQRRFMIVKDAAKMQSFSGYKSVDVASNIKKETAFIF CHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHH LQKLNDLPGIDVSTQPIRVYPYKEVGSAVLGYISKISGDNEKYKEKGYDPSSDYIGIDGI HHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEECCCCH EGVFEERLKGSKGGRIVKLNKTGRVIEELGRREPYPGQNIQLTIDTNIQKAAEESLDSVM HHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHH KDLQQRGVQGDVNTSNATRGAAVALDVNTGEILALASRPGFDPNLFATPGGLTPELYKQY HHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEECCCCCCHHHHHHH FNPDLTEFGKQYILNKGLMGYYPGNTLDEVLEKLFPLDKSIKNNKTMRQDLYDIYPKPFY CCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCC NYATMSVVPPGSTFKAMTAIAGLESGVITPGYSISDTGVFDDGKKFVKKFAVGGYGSVDL CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH YRGLEVSSNPYFMTVGKLLRESFGDDILAKYAWKFGLGVPPNSDEKASTGIEIPEKFGQV HCCCCCCCCCEEEHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH FNTYTLSNVYATQYLWQTMSTLKSGTDARGNKFPSINLYDSEKDSDKVKELKKQIKNSIQ HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH DSIKEGTKKFDSNNYIKMLTELVNQDPIYKGKNVSKDQIKRIVDVIYYVTISDAHFQLGV HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCEEEEEC GANMYNAAIGQGISNFTPLQLANFVSTIANGGDRYKLHLVKEIKDADGNVVETVKPEIVE CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCEEECCCHHHHH KTNVSKENLAAVRQGMARVNEGTDGTAAAVFKGFPIQTAGKTGSSTFSNSQDYYGRTSYG HCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE VYIGFAPVDNPKIAVCVIVFDGGHGGSVAPVAKAMYEAYFKKELNKDKPDAAKASNSGQK EEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC ENTKNSDASGDDAKDIENKNTNSR CCCCCCCCCCCCHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]