| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is ppdK [H]
Identifier: 148380893
GI number: 148380893
Start: 3131704
End: 3134337
Strand: Reverse
Name: ppdK [H]
Synonym: CBO2942
Alternate gene names: 148380893
Gene position: 3134337-3131704 (Counterclockwise)
Preceding gene: 148380894
Following gene: 148380891
Centisome position: 80.64
GC content: 33.83
Gene sequence:
>2634_bases ATGGAAAATAAGAAGCATGTATATCTTTTTAGTGAAGGAAATGCATCAATGAGAGAGTTGTTAGGGGGAAAGGGTGCTAA TCTAGCAGAAATGACTAATTTAGGAATCCCTGTTCCAACAGGATTCACAGTAACTACAGAGGCTTGTATAAAATATTATA AGGATGGTAAAAAGTTAGCAGACGAAGTAGTACAGCAAATAACAGATGCTATGAGAGAAGTGGAAAAGGAAACTAACAAA AAATTTGGAAGTGAAGAAAATCCTTTGTTAGTTTCAGTAAGATCAGGGGCTAGGGTTTCTATGCCAGGAATGATGGATAC TATATTAAATTTAGGATTAAATGACAAAACAGTAGAATCATTAAGCAAACTTACAAATAATGAAAGATTTGCTTATGACT CCTATAGAAGATTTATTCAAATGTTTTCAGATGTAGTTATGGGAATAGATAAAAGAGATTTTGAAGATGTATTAGATCAA ATGAAAGAAGAAAAGGGTGTTGACTACGATACAGATTTAACATCAGAAGATTTGAAAAAAATTGTAGTTAAATTTAAAGA AATATATAAAAAAGAAATGAGAGAAGATTTTCCACAAGAACCTAAAGAGCAACTATTAGCAGCTGTTACAGCAGTATTTG GTTCATGGGATAACCCAAGAGCTATAGTGTACAGAAGGCTAAATGATATTCCAGGAGATTGGGGTACAGCTGTAAATGTA CAATCAATGGTATTTGGAAATATGGGAGAAACTTCAGGAACAGGAGTTGCCTTTACAAGAAATCCCTCTACTGGAGAAAA TAAAATATTTGGCGAATATTTAATAAATGCTCAAGGTGAAGACGTTGTTGCTGGAATTAGAACGCCTCAACCTATAGCAA AACTAAAAGAAGATTTACCACAATGTTATGAAGAGTTTATGAGTATTGCTAGAAAGCTTGAAAAGCATTACAAAGATATG CAAGATATGGAGTTTACTATAGAACAAGGGAAATTATACTTCCTTCAAACTAGAAATGGTAAAAGGACTGCTCAATCTGC ATTAAAAATAGCTGTAGATTTAGTAGAAGAAGGAACATTACAAAAAGAAGAAGCCTTATTAAAAGTAGATCCAAAGCAAT TAGATACATTATTACATCCTAATTTTGATGAAAAAGAATTAAAAGCTGCAGAAGTTATAGCTACTGGTCTTCCAGCATCC CCAGGAGCAGCTTGTGGAAGAGTTTATTTTACAGCTGAAGAGGCTAAAATTCATCATGAAAAAGGCGAAAAAGTAATATT AGTAAGACTTGAAACATCCCCAGAAGATATAGAAGGAATGGTAGCAGCAGAAGGAATATTAACAGCAAGAGGTGGAATGA CATCTCATGCAGCAGTTGTTGCTAGAGGTATGGGAACATGTTGTGTAGCAGGATGTTCTGAAATAACTATAAATGAAAAA GAAAAATATTTTCATGCAGCAGGAAAATCTTATAAAGAAGGAGACTATATATCTCTAGATGGAAGTACAGGAAAGGTTTA TGGTAAATCCATAAAAACTGTAGCACCAGAAATAAGTGGATACTTTGGAACCTTTATGGAATGGGCTGATAAAGTAAGAG TATTGAAAGTTAGAACTAATGCGGATGCTCCAAGAGATGCAGCTCAAGCAGTTACCTTTGGAGCAGAAGGAATAGGTCTT TGTAGAACAGAACACATGTTCTTCCAAGAAGAGAGAATACCGGCTGTTAGAGAAATGATACTTGCTAAAACAGAGGCTCA AAGAAGGAAGGCTTTAGGTAAGCTATTACCAATGCAAAGAGAAGATTTTGTAGGAATATATGAAGCCATGGCAGAAAAGC CAGTGACTGTAAGATTATTAGATCCACCTTTACATGAGTTTTTACCAAAAGATGATGAGGATATAATAGAATTATCAAAA GAAATGGAAGTAAGTTTTGAAGAATTAAAGAATACTGTACTATCATTACATGAATTCAATCCAATGATGGGGCATAGAGG TTGTCGTTTAACTGTGTCTTATCCAGAAATGGCAGAAATGCAAACTACAGCTATAATAGAAGCTGCTATAGAAGTTACAA GAAATAAAGGAATAAAAATTAAGCCAGAAATAATGATACCGCTAATAGGAGAAATAAAAGAAATGGCTTATGTAAAATCT ATAGTTGTAGAAACAGCAGATAAGATACTAGAGAGAGAAAATGTAGAAATGGAATACCAAGTTGGAACAATGATAGAAAT TCCAAGAGCTGCACTTACAGCTGATGAAATAGCTAAAGAAGCAGAATTCTTTTCTTTTGGAACAAATGATTTGACACAAA TGACCTTTGGATTTTCAAGAGATGATGCAGGTAAATTCTTAAATTCTTATTATGATAAAAAAATATATGAGTTTGATCCA TTCCAAAGAATAGACCAAACTGGTGTTGGAAAGCTTGTGGAAATGGCAGTAAAACTAGGAAAACAAATAAGACCAGATAT AAAATTGGGAATATGTGGAGAACATGGTGGAGATCCATCATCTATAGAATTCTGTCACAATGTAGGATTAAACTATGTGT CTTGTTCACCATTTAGGGTACCTGTAGCAAGATTAGCTGCAGCACAAGCTCAAATAAAAAACCCAAGAAAGTAA
Upstream 100 bases:
>100_bases AAGGTTGGAGAAAAAGTTTAAGATAAAAGTTTCGAACAGATAATTACTTATCTGAATATATATTAAATTACAAAGAAATT TTACAGAGGGGATGTTTATT
Downstream 100 bases:
>100_bases AAGTATAAGTATAAGGGATGTAAGCCTAGTGTAAAATTGGCTTACATCTTTTTTAATATATTAAATCTCTGCCAAATCTA GATTCTATATGTTTTCCAAA
Product: pyruvate phosphate dikinase
Products: NA
Alternate protein names: Pyruvate, orthophosphate dikinase [H]
Number of amino acids: Translated: 877; Mature: 877
Protein sequence:
>877_residues MENKKHVYLFSEGNASMRELLGGKGANLAEMTNLGIPVPTGFTVTTEACIKYYKDGKKLADEVVQQITDAMREVEKETNK KFGSEENPLLVSVRSGARVSMPGMMDTILNLGLNDKTVESLSKLTNNERFAYDSYRRFIQMFSDVVMGIDKRDFEDVLDQ MKEEKGVDYDTDLTSEDLKKIVVKFKEIYKKEMREDFPQEPKEQLLAAVTAVFGSWDNPRAIVYRRLNDIPGDWGTAVNV QSMVFGNMGETSGTGVAFTRNPSTGENKIFGEYLINAQGEDVVAGIRTPQPIAKLKEDLPQCYEEFMSIARKLEKHYKDM QDMEFTIEQGKLYFLQTRNGKRTAQSALKIAVDLVEEGTLQKEEALLKVDPKQLDTLLHPNFDEKELKAAEVIATGLPAS PGAACGRVYFTAEEAKIHHEKGEKVILVRLETSPEDIEGMVAAEGILTARGGMTSHAAVVARGMGTCCVAGCSEITINEK EKYFHAAGKSYKEGDYISLDGSTGKVYGKSIKTVAPEISGYFGTFMEWADKVRVLKVRTNADAPRDAAQAVTFGAEGIGL CRTEHMFFQEERIPAVREMILAKTEAQRRKALGKLLPMQREDFVGIYEAMAEKPVTVRLLDPPLHEFLPKDDEDIIELSK EMEVSFEELKNTVLSLHEFNPMMGHRGCRLTVSYPEMAEMQTTAIIEAAIEVTRNKGIKIKPEIMIPLIGEIKEMAYVKS IVVETADKILERENVEMEYQVGTMIEIPRAALTADEIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLNSYYDKKIYEFDP FQRIDQTGVGKLVEMAVKLGKQIRPDIKLGICGEHGGDPSSIEFCHNVGLNYVSCSPFRVPVARLAAAQAQIKNPRK
Sequences:
>Translated_877_residues MENKKHVYLFSEGNASMRELLGGKGANLAEMTNLGIPVPTGFTVTTEACIKYYKDGKKLADEVVQQITDAMREVEKETNK KFGSEENPLLVSVRSGARVSMPGMMDTILNLGLNDKTVESLSKLTNNERFAYDSYRRFIQMFSDVVMGIDKRDFEDVLDQ MKEEKGVDYDTDLTSEDLKKIVVKFKEIYKKEMREDFPQEPKEQLLAAVTAVFGSWDNPRAIVYRRLNDIPGDWGTAVNV QSMVFGNMGETSGTGVAFTRNPSTGENKIFGEYLINAQGEDVVAGIRTPQPIAKLKEDLPQCYEEFMSIARKLEKHYKDM QDMEFTIEQGKLYFLQTRNGKRTAQSALKIAVDLVEEGTLQKEEALLKVDPKQLDTLLHPNFDEKELKAAEVIATGLPAS PGAACGRVYFTAEEAKIHHEKGEKVILVRLETSPEDIEGMVAAEGILTARGGMTSHAAVVARGMGTCCVAGCSEITINEK EKYFHAAGKSYKEGDYISLDGSTGKVYGKSIKTVAPEISGYFGTFMEWADKVRVLKVRTNADAPRDAAQAVTFGAEGIGL CRTEHMFFQEERIPAVREMILAKTEAQRRKALGKLLPMQREDFVGIYEAMAEKPVTVRLLDPPLHEFLPKDDEDIIELSK EMEVSFEELKNTVLSLHEFNPMMGHRGCRLTVSYPEMAEMQTTAIIEAAIEVTRNKGIKIKPEIMIPLIGEIKEMAYVKS IVVETADKILERENVEMEYQVGTMIEIPRAALTADEIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLNSYYDKKIYEFDP FQRIDQTGVGKLVEMAVKLGKQIRPDIKLGICGEHGGDPSSIEFCHNVGLNYVSCSPFRVPVARLAAAQAQIKNPRK >Mature_877_residues MENKKHVYLFSEGNASMRELLGGKGANLAEMTNLGIPVPTGFTVTTEACIKYYKDGKKLADEVVQQITDAMREVEKETNK KFGSEENPLLVSVRSGARVSMPGMMDTILNLGLNDKTVESLSKLTNNERFAYDSYRRFIQMFSDVVMGIDKRDFEDVLDQ MKEEKGVDYDTDLTSEDLKKIVVKFKEIYKKEMREDFPQEPKEQLLAAVTAVFGSWDNPRAIVYRRLNDIPGDWGTAVNV QSMVFGNMGETSGTGVAFTRNPSTGENKIFGEYLINAQGEDVVAGIRTPQPIAKLKEDLPQCYEEFMSIARKLEKHYKDM QDMEFTIEQGKLYFLQTRNGKRTAQSALKIAVDLVEEGTLQKEEALLKVDPKQLDTLLHPNFDEKELKAAEVIATGLPAS PGAACGRVYFTAEEAKIHHEKGEKVILVRLETSPEDIEGMVAAEGILTARGGMTSHAAVVARGMGTCCVAGCSEITINEK EKYFHAAGKSYKEGDYISLDGSTGKVYGKSIKTVAPEISGYFGTFMEWADKVRVLKVRTNADAPRDAAQAVTFGAEGIGL CRTEHMFFQEERIPAVREMILAKTEAQRRKALGKLLPMQREDFVGIYEAMAEKPVTVRLLDPPLHEFLPKDDEDIIELSK EMEVSFEELKNTVLSLHEFNPMMGHRGCRLTVSYPEMAEMQTTAIIEAAIEVTRNKGIKIKPEIMIPLIGEIKEMAYVKS IVVETADKILERENVEMEYQVGTMIEIPRAALTADEIAKEAEFFSFGTNDLTQMTFGFSRDDAGKFLNSYYDKKIYEFDP FQRIDQTGVGKLVEMAVKLGKQIRPDIKLGICGEHGGDPSSIEFCHNVGLNYVSCSPFRVPVARLAAAQAQIKNPRK
Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1788756, Length=455, Percent_Identity=24.3956043956044, Blast_Score=103, Evalue=4e-23, Organism=Escherichia coli, GI1787994, Length=741, Percent_Identity=24.1565452091768, Blast_Score=100, Evalue=7e-22, Organism=Escherichia coli, GI48994992, Length=451, Percent_Identity=23.5033259423503, Blast_Score=89, Evalue=1e-18, Organism=Escherichia coli, GI1788726, Length=447, Percent_Identity=23.2662192393736, Blast_Score=83, Evalue=7e-17, Organism=Escherichia coli, GI1789193, Length=434, Percent_Identity=22.5806451612903, Blast_Score=82, Evalue=1e-16,
Paralogues:
None
Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR018274 - InterPro: IPR023151 - InterPro: IPR000121 - InterPro: IPR002192 - InterPro: IPR010121 - InterPro: IPR015813 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]
EC number: =2.7.9.1 [H]
Molecular weight: Translated: 98010; Mature: 98010
Theoretical pI: Translated: 5.04; Mature: 5.04
Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 4.0 %Met (Translated Protein) 5.2 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 4.0 %Met (Mature Protein) 5.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MENKKHVYLFSEGNASMRELLGGKGANLAEMTNLGIPVPTGFTVTTEACIKYYKDGKKLA CCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEHHHHHHHHHHCHHHHH DEVVQQITDAMREVEKETNKKFGSEENPLLVSVRSGARVSMPGMMDTILNLGLNDKTVES HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHH LSKLTNNERFAYDSYRRFIQMFSDVVMGIDKRDFEDVLDQMKEEKGVDYDTDLTSEDLKK HHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH IVVKFKEIYKKEMREDFPQEPKEQLLAAVTAVFGSWDNPRAIVYRRLNDIPGDWGTAVNV HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEH QSMVFGNMGETSGTGVAFTRNPSTGENKIFGEYLINAQGEDVVAGIRTPQPIAKLKEDLP HHHHCCCCCCCCCCCEEEECCCCCCCCCEEHHHHCCCCCCCEEEECCCCHHHHHHHHHHH QCYEEFMSIARKLEKHYKDMQDMEFTIEQGKLYFLQTRNGKRTAQSALKIAVDLVEEGTL HHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCC QKEEALLKVDPKQLDTLLHPNFDEKELKAAEVIATGLPASPGAACGRVYFTAEEAKIHHE CHHHHHHEECHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHC KGEKVILVRLETSPEDIEGMVAAEGILTARGGMTSHAAVVARGMGTCCVAGCSEITINEK CCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCHHHHCCCCCEEECHH EKYFHAAGKSYKEGDYISLDGSTGKVYGKSIKTVAPEISGYFGTFMEWADKVRVLKVRTN HHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCHHHHHHHHHHHHHHCCEEEEEEECC ADAPRDAAQAVTFGAEGIGLCRTEHMFFQEERIPAVREMILAKTEAQRRKALGKLLPMQR CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC EDFVGIYEAMAEKPVTVRLLDPPLHEFLPKDDEDIIELSKEMEVSFEELKNTVLSLHEFN HHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC PMMGHRGCRLTVSYPEMAEMQTTAIIEAAIEVTRNKGIKIKPEIMIPLIGEIKEMAYVKS CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEECCHHHHHHHHHHHHH IVVETADKILERENVEMEYQVGTMIEIPRAALTADEIAKEAEFFSFGTNDLTQMTFGFSR HHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC DDAGKFLNSYYDKKIYEFDPFQRIDQTGVGKLVEMAVKLGKQIRPDIKLGICGEHGGDPS CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCH SIEFCHNVGLNYVSCSPFRVPVARLAAAQAQIKNPRK HHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCC >Mature Secondary Structure MENKKHVYLFSEGNASMRELLGGKGANLAEMTNLGIPVPTGFTVTTEACIKYYKDGKKLA CCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEHHHHHHHHHHCHHHHH DEVVQQITDAMREVEKETNKKFGSEENPLLVSVRSGARVSMPGMMDTILNLGLNDKTVES HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHH LSKLTNNERFAYDSYRRFIQMFSDVVMGIDKRDFEDVLDQMKEEKGVDYDTDLTSEDLKK HHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHH IVVKFKEIYKKEMREDFPQEPKEQLLAAVTAVFGSWDNPRAIVYRRLNDIPGDWGTAVNV HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEH QSMVFGNMGETSGTGVAFTRNPSTGENKIFGEYLINAQGEDVVAGIRTPQPIAKLKEDLP HHHHCCCCCCCCCCCEEEECCCCCCCCCEEHHHHCCCCCCCEEEECCCCHHHHHHHHHHH QCYEEFMSIARKLEKHYKDMQDMEFTIEQGKLYFLQTRNGKRTAQSALKIAVDLVEEGTL HHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCC QKEEALLKVDPKQLDTLLHPNFDEKELKAAEVIATGLPASPGAACGRVYFTAEEAKIHHE CHHHHHHEECHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHC KGEKVILVRLETSPEDIEGMVAAEGILTARGGMTSHAAVVARGMGTCCVAGCSEITINEK CCCEEEEEEECCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCHHHHCCCCCEEECHH EKYFHAAGKSYKEGDYISLDGSTGKVYGKSIKTVAPEISGYFGTFMEWADKVRVLKVRTN HHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCHHHHHHHHHHHHHHCCEEEEEEECC ADAPRDAAQAVTFGAEGIGLCRTEHMFFQEERIPAVREMILAKTEAQRRKALGKLLPMQR CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC EDFVGIYEAMAEKPVTVRLLDPPLHEFLPKDDEDIIELSKEMEVSFEELKNTVLSLHEFN HHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC PMMGHRGCRLTVSYPEMAEMQTTAIIEAAIEVTRNKGIKIKPEIMIPLIGEIKEMAYVKS CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEECCHHHHHHHHHHHHH IVVETADKILERENVEMEYQVGTMIEIPRAALTADEIAKEAEFFSFGTNDLTQMTFGFSR HHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC DDAGKFLNSYYDKKIYEFDPFQRIDQTGVGKLVEMAVKLGKQIRPDIKLGICGEHGGDPS CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCH SIEFCHNVGLNYVSCSPFRVPVARLAAAQAQIKNPRK HHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]