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The map label for this gene is ygcA [C]

Identifier: 148380817

GI number: 148380817

Start: 3038632

End: 3040002

Strand: Reverse

Name: ygcA [C]

Synonym: CBO2859

Alternate gene names: 148380817

Gene position: 3040002-3038632 (Counterclockwise)

Preceding gene: 148380818

Following gene: 148380816

Centisome position: 78.21

GC content: 30.27

Gene sequence:

>1371_bases
ATGAAAAATAAATATATGAGAAAAGGCAAGGAGTATGAATTTCTTATAGAAGAAACAGAATTTCCGGGAACAGGTGTAGC
ACAAAAGGATGGTGTGCCAGTTTATATAAAAGGAACAGTACCAGGACAAAAGGTTTTAGCGAAAGTAACTAAAAAAAGAA
GAGAATATGCCCAGGCTAAACTTTTAGAAATAATAGAAAATATAGATTATGCAATAGAAAATAAATGTCCTCATTTTGGT
CAATGTGGAGGGTGTTCAACCCAATACATACCTTATGAAAAACAACTACAAATTAAAGAAGAACAGCTGTTAAAATTATT
TAAAAGTAAAGAAATAAAAGGATTTGATTTTTTAGGAGTAGAAAAAAGCCCAGAGGAATACGAATATAGAAACAAGATGG
AGTTTACCTTTGGGGATATGGAAAAGGGCGGAGATCTTACTTTAGGAATGCATGTTAAAAATAGAAATTTTTCTATTGTA
ACTGTGGATAACTGTGAAATTGTAGATAGAGATTTTAGAAATATATTAACAACTGTGGTTAATTATTTTAATGAAAAAAG
ATTACCTAAATACAGAGTAATGAGTCATGAAGGTTTTTTAAGAAATTTAGTTATAAGAAAAGCTAAAAATACAGGAGAGA
TACTAGTAAACATAGTAACTACATCTCAAATGGAATTTGATTTTAAAGAAATAGTAGATATGTTATTAAAGGTGGAATGC
AAAGGAGAAATAAAAGGCATTCTTCATACTATAAATGATACATTATCTGATGTAGTTCAGGTGGATAAATTAGAAATATT
ATATGGAAGAGACTATATAATAGAAGAGTTATTAGGACTAAAATTTAAGATAGCTCCAGAAGCCTTTTTTCAAACAAATT
CAAAGGGAGCAGAAAAACTATATAGTATAGTAAAAGACTTCTTAGGTGATGCTAGTTCAAAAGTGGTTTTCGACCTTTAT
TGTGGTACAGGAACCATAGGACAAATAGTTGCTCCAAAGGCTAAAAAAGTTATAGGTGTGGAATTAATAGAAGAAGCAGT
TAAGGCAGCTAATGAAAATGCAAAATTAAATGGACTAAATAACTGTGAATTTATAGCTGGTGATGTAGCAAAGGTTATAA
AAGATGTAAAACAAAAGCCAGATATAATTATATTGGATCCACCAAGACCAGGTGTACATCCAGTAGCTTTAGAATATGTG
GTTAAGTTTGAGCCAAAGGAAATAATCTATGTATCTTGTAATCCAAAGACTTTAGTAGATGATTTAAAATATCTTATAGA
TAATGGGTATAAATTAGAAAAAGTTAAGGGCATGGACATGTTTCCTCATACACCTCATGTAGAGTGTGTTGTGAAGATAG
AGAAGAAATAG

Upstream 100 bases:

>100_bases
AGTCTATTATTTATAGTAGCTACAGAAAATGGTATTAATAGTATAGCAATAAAATAAAATCTAATAAATAAAATACTTAA
AATAAATTTACTCTGAAAGG

Downstream 100 bases:

>100_bases
ATTTTCTTGATTCACTCTTTATTGACTTTTTAGACGTATAATAGTATAATGATAGAAAAATTGAAATTTGAGGTGAAAAA
ATGATTAATTTTTCTTGCTA

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFG
QCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIV
TVDNCEIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVEC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLY
CGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYV
VKFEPKEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVECVVKIEKK

Sequences:

>Translated_456_residues
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFG
QCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIV
TVDNCEIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVEC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLY
CGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYV
VKFEPKEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVECVVKIEKK
>Mature_456_residues
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAKLLEIIENIDYAIENKCPHFG
QCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGVEKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIV
TVDNCEIVDRDFRNILTTVVNYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVEC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKLYSIVKDFLGDASSKVVFDLY
CGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLNNCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYV
VKFEPKEIIYVSCNPKTLVDDLKYLIDNGYKLEKVKGMDMFPHTPHVECVVKIEKK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=360, Percent_Identity=29.7222222222222, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI51173878, Length=360, Percent_Identity=29.7222222222222, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI269784680, Length=358, Percent_Identity=29.608938547486, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI269784676, Length=358, Percent_Identity=29.608938547486, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI20149688, Length=358, Percent_Identity=29.608938547486, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI269784678, Length=352, Percent_Identity=29.5454545454545, Blast_Score=145, Evalue=9e-35,
Organism=Escherichia coli, GI1789148, Length=443, Percent_Identity=27.9909706546275, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787083, Length=379, Percent_Identity=25.3298153034301, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790403, Length=232, Percent_Identity=26.2931034482759, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=551, Percent_Identity=25.5898366606171, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322909, Length=410, Percent_Identity=27.3170731707317, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24666579, Length=433, Percent_Identity=28.8683602771363, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51899; Mature: 51899

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAK
CCCCHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LLEIIENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGV
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECC
EKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNCEIVDRDFRNILTTVV
CCCCHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
NYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVEC
HHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHEECC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKL
CHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHH
YSIVKDFLGDASSKVVFDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLN
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCC
NCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVD
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHH
DLKYLIDNGYKLEKVKGMDMFPHTPHVECVVKIEKK
HHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKNKYMRKGKEYEFLIEETEFPGTGVAQKDGVPVYIKGTVPGQKVLAKVTKKRREYAQAK
CCCCHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LLEIIENIDYAIENKCPHFGQCGGCSTQYIPYEKQLQIKEEQLLKLFKSKEIKGFDFLGV
HHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEECC
EKSPEEYEYRNKMEFTFGDMEKGGDLTLGMHVKNRNFSIVTVDNCEIVDRDFRNILTTVV
CCCCHHHHHCCCEEEEECCCCCCCCEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
NYFNEKRLPKYRVMSHEGFLRNLVIRKAKNTGEILVNIVTTSQMEFDFKEIVDMLLKVEC
HHHHHCCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHEECC
KGEIKGILHTINDTLSDVVQVDKLEILYGRDYIIEELLGLKFKIAPEAFFQTNSKGAEKL
CHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCEEEECCHHHHCCCCHHHHHH
YSIVKDFLGDASSKVVFDLYCGTGTIGQIVAPKAKKVIGVELIEEAVKAANENAKLNGLN
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCC
NCEFIAGDVAKVIKDVKQKPDIIILDPPRPGVHPVALEYVVKFEPKEIIYVSCNPKTLVD
CCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCEEEEEECCCHHHHH
DLKYLIDNGYKLEKVKGMDMFPHTPHVECVVKIEKK
HHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]