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The map label for this gene is cfr

Identifier: 148380815

GI number: 148380815

Start: 3035493

End: 3036527

Strand: Reverse

Name: cfr

Synonym: CBO2857

Alternate gene names: 148380815

Gene position: 3036527-3035493 (Counterclockwise)

Preceding gene: 148380816

Following gene: 148380814

Centisome position: 78.12

GC content: 32.08

Gene sequence:

>1035_bases
ATGAAACAGACAAAAACGAAATATGGAAAAATGAAACAAATAGCATCGAATTTAAAATTACCTGATTATAGATACGAACA
GCTTACAAAAGCTATTTTTCATCAAAGAATAGATAATTTTCATGATATGCATATACTACCAAAAGCGTTAAGGATAGCTT
TAGTAAATGAGTTTGGAAAGAATGTATCTAGTGTAACACCTATTTTTTCACAAGATTCTAAACAAGCTCAAAAGTTGTTA
TTTGAATTGACTGATGGAGAAAGAATAGAAGCCGTTGGACTAAAGTATAAACAGGGGTGGGAATCGTTTTGTATTTCTTC
CCAATGTGGTTGTAGTTTTGGATGTCGTTTTTGTGCAACGGGAAGTGCTGGATTTAAACGCAATCTTACTGCTGATGAGA
TAACTGACCAATTACTTTATTTCTATTTTAATGACCATAGATTGAATAGTATTTCATTTATGGGAATGGGTGAGGCTTTT
GCAAATCCAGAGTTATTTGATGCAGTAAAAATTTTAACTGATCAAAATTTATTTGGGTTAAGTCAACGAAGAATTACTAT
TTCAACAATTGGCATTATACCAGGAATTCAAAGACTGACTAAAGAATTTCCACAAGTGAATCTGGCTTTTTCACTTCATT
CACCATTTGAAAGTCAACGAAGCGATTTAATGCCTATAAATAAAAGATTTCCATTGAATGAGGTAATGAAGACATTAGAT
GAACATATCATTCATACGGGACGACGAGTGTTTATTGCTTATATTATGCTTGAAGGAATTAATGATTCGAAAGAACATGC
AGAGGCAATTATAGGTTTATTGAGAAATCGTGGTTCATGGGAGCATTTATATCACATTGATTTGATACCTTATAATTCTA
CGGACAAAACAACTTTTAAATTTCAATCTTCAAGTGCTATCAAGCAATTTTGCAGTACACTAAAGAAAGCTAGTATTAGT
GCAACTGTTAGAACACAATTTGGTTCTGAAATTAGTGCTGCTTGCGGACAATTGTGTTATGAAAATGAATTATGA

Upstream 100 bases:

>100_bases
GCAAAGCCAGATGTTTTGGGCGACGCTTGCATGGAGTAGTATAAGTCGCTAATTATACTGGCTTTGCTTCTTAACTATTA
ATAGTGAGGAGGAAGCCTAC

Downstream 100 bases:

>100_bases
TGTTAGTTCAAAAATATCCATTTAAGTAGAGAAATAGTCTCATCTTAAAGATCTTGAAGATTTTTAATTAAATATTAAAG
ACTATTGTAGGTCCCTCAGA

Product: chloramphenicol/florfenicol resistance protein

Products: NA

Alternate protein names: 23S rRNA m8A2503 methyltransferase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKQTKTKYGKMKQIASNLKLPDYRYEQLTKAIFHQRIDNFHDMHILPKALRIALVNEFGKNVSSVTPIFSQDSKQAQKLL
FELTDGERIEAVGLKYKQGWESFCISSQCGCSFGCRFCATGSAGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAF
ANPELFDAVKILTDQNLFGLSQRRITISTIGIIPGIQRLTKEFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
EHIIHTGRRVFIAYIMLEGINDSKEHAEAIIGLLRNRGSWEHLYHIDLIPYNSTDKTTFKFQSSSAIKQFCSTLKKASIS
ATVRTQFGSEISAACGQLCYENEL

Sequences:

>Translated_344_residues
MKQTKTKYGKMKQIASNLKLPDYRYEQLTKAIFHQRIDNFHDMHILPKALRIALVNEFGKNVSSVTPIFSQDSKQAQKLL
FELTDGERIEAVGLKYKQGWESFCISSQCGCSFGCRFCATGSAGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAF
ANPELFDAVKILTDQNLFGLSQRRITISTIGIIPGIQRLTKEFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
EHIIHTGRRVFIAYIMLEGINDSKEHAEAIIGLLRNRGSWEHLYHIDLIPYNSTDKTTFKFQSSSAIKQFCSTLKKASIS
ATVRTQFGSEISAACGQLCYENEL
>Mature_344_residues
MKQTKTKYGKMKQIASNLKLPDYRYEQLTKAIFHQRIDNFHDMHILPKALRIALVNEFGKNVSSVTPIFSQDSKQAQKLL
FELTDGERIEAVGLKYKQGWESFCISSQCGCSFGCRFCATGSAGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAF
ANPELFDAVKILTDQNLFGLSQRRITISTIGIIPGIQRLTKEFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
EHIIHTGRRVFIAYIMLEGINDSKEHAEAIIGLLRNRGSWEHLYHIDLIPYNSTDKTTFKFQSSSAIKQFCSTLKKASIS
ATVRTQFGSEISAACGQLCYENEL

Specific function: Specifically methylates position 8 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family. Cfr subfamily

Homologues:

Organism=Escherichia coli, GI1788865, Length=346, Percent_Identity=31.7919075144509, Blast_Score=164, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CFR_CLOB1 (A7FX96)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385100.1
- STRING:   A7FX96
- GeneID:   5398193
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2801
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   SSQCGCG
- ProtClustDB:   PRK14453
- BioCyc:   CBOT441770:CLB_2801-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01873
- InterPro:   IPR007197
- InterPro:   IPR022881
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.194

Molecular weight: Translated: 39161; Mature: 39161

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTKTKYGKMKQIASNLKLPDYRYEQLTKAIFHQRIDNFHDMHILPKALRIALVNEFGK
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NVSSVTPIFSQDSKQAQKLLFELTDGERIEAVGLKYKQGWESFCISSQCGCSFGCRFCAT
CHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHCHHHHHCCCCCCCCCCCEEEEC
GSAGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAFANPELFDAVKILTDQNLFGL
CCCCCCCCCCHHHHHHHHEEEEECCCCCCEEEEEECCHHHCCCHHHHHHHHHHCCCCCCC
SQRRITISTIGIIPGIQRLTKEFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
CCCEEEEEEHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHH
EHIIHTGRRVFIAYIMLEGINDSKEHAEAIIGLLRNRGSWEHLYHIDLIPYNSTDKTTFK
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
FQSSSAIKQFCSTLKKASISATVRTQFGSEISAACGQLCYENEL
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTKTKYGKMKQIASNLKLPDYRYEQLTKAIFHQRIDNFHDMHILPKALRIALVNEFGK
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NVSSVTPIFSQDSKQAQKLLFELTDGERIEAVGLKYKQGWESFCISSQCGCSFGCRFCAT
CHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHCHHHHHCCCCCCCCCCCEEEEC
GSAGFKRNLTADEITDQLLYFYFNDHRLNSISFMGMGEAFANPELFDAVKILTDQNLFGL
CCCCCCCCCCHHHHHHHHEEEEECCCCCCEEEEEECCHHHCCCHHHHHHHHHHCCCCCCC
SQRRITISTIGIIPGIQRLTKEFPQVNLAFSLHSPFESQRSDLMPINKRFPLNEVMKTLD
CCCEEEEEEHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCCCCHHHHHHHHH
EHIIHTGRRVFIAYIMLEGINDSKEHAEAIIGLLRNRGSWEHLYHIDLIPYNSTDKTTFK
HHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEE
FQSSSAIKQFCSTLKKASISATVRTQFGSEISAACGQLCYENEL
ECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA