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The map label for this gene is sigK [H]

Identifier: 148380498

GI number: 148380498

Start: 2679478

End: 2680182

Strand: Reverse

Name: sigK [H]

Synonym: CBO2541

Alternate gene names: 148380498

Gene position: 2680182-2679478 (Counterclockwise)

Preceding gene: 148380499

Following gene: 148380497

Centisome position: 68.95

GC content: 29.36

Gene sequence:

>705_bases
GTGTTCTTTTTAAGTTACTTATGGGATGTACTAGGAAGTGCATTATTTTTAACAGGCTATGTTACAGGAAATACTTCTTT
CCCCAAGCCTCTAAGTGATGAAGAAGAAAAATACTATTTGGATAGGTTAAAAAAGGGTGATGCTTTAGCAAAAGATATCT
TAGTGGAAAGAAATTTAAGATTAGTGGCTCACATAGTAAAAAAATATTCTTATCCGGGAAAAGATATTGATGATTTAATA
TCTATAGGAACTGTAGGTCTTATAAAAGCTATAGACTCTTTTGATAATTCAAAGGGTACTAGACTTGCCACTTATGCAGC
AAGATGTATAGAAAATGAAATATTAATGCTCATAAGAAATAATAAAAAAACTAAAGGTGAGGTATATCTCCAAGATCCTA
TAGGAGTAGACAAAGAAGGAAATGAAATTTCTCTTATGGATATATTAAGTAGTGATGAAGATTCTATAATAGAAATAGTT
TCAACGAAAATAGAAGTAAAAAAATTATATGGAAAAATAGATACTTGCCTTAGGGGAAGGGAAAAAAAGGTAATACAAAT
GAGGTATGGGTTAAAGGATGGAAGACCCAGAACTCAAAGAGAAATAGCTGGTATACTAAACATATCTAGATCTTATGTTT
CACGCATAGAAAAAAAAGCCCTTAAAAAGCTTTATAAAGAATTAAATTATAATAAAAATATTTAA

Upstream 100 bases:

>100_bases
ATTGTCTTTTAATGCCTAATGGAAAATATGATTTTAAATATGCAACTAGTACAAGCTTATTGAATATTATATTAATAAGC
ACTATTAGGAGGAGCAGCTT

Downstream 100 bases:

>100_bases
ATAAGAACTCTAAAGAATTAAGAATTTTATACATATAAGGTGATTCTTAATTCTTTTGTTTTATTTTTTATTTATTTTAT
GGCAGGAGATTCTTTACAGA

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLRLVAHIVKKYSYPGKDIDDLI
SIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRNNKKTKGEVYLQDPIGVDKEGNEISLMDILSSDEDSIIEIV
STKIEVKKLYGKIDTCLRGREKKVIQMRYGLKDGRPRTQREIAGILNISRSYVSRIEKKALKKLYKELNYNKNI

Sequences:

>Translated_234_residues
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLRLVAHIVKKYSYPGKDIDDLI
SIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRNNKKTKGEVYLQDPIGVDKEGNEISLMDILSSDEDSIIEIV
STKIEVKKLYGKIDTCLRGREKKVIQMRYGLKDGRPRTQREIAGILNISRSYVSRIEKKALKKLYKELNYNKNI
>Mature_234_residues
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLRLVAHIVKKYSYPGKDIDDLI
SIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRNNKKTKGEVYLQDPIGVDKEGNEISLMDILSSDEDSIIEIV
STKIEVKKLYGKIDTCLRGREKKVIQMRYGLKDGRPRTQREIAGILNISRSYVSRIEKKALKKLYKELNYNKNI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=254, Percent_Identity=24.8031496062992, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26713; Mature: 26713

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLR
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCHH
LVAHIVKKYSYPGKDIDDLISIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEC
NKKTKGEVYLQDPIGVDKEGNEISLMDILSSDEDSIIEIVSTKIEVKKLYGKIDTCLRGR
CCCCCCEEEEECCCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKKVIQMRYGLKDGRPRTQREIAGILNISRSYVSRIEKKALKKLYKELNYNKNI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFFLSYLWDVLGSALFLTGYVTGNTSFPKPLSDEEEKYYLDRLKKGDALAKDILVERNLR
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCHH
LVAHIVKKYSYPGKDIDDLISIGTVGLIKAIDSFDNSKGTRLATYAARCIENEILMLIRN
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEC
NKKTKGEVYLQDPIGVDKEGNEISLMDILSSDEDSIIEIVSTKIEVKKLYGKIDTCLRGR
CCCCCCEEEEECCCCCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKKVIQMRYGLKDGRPRTQREIAGILNISRSYVSRIEKKALKKLYKELNYNKNI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]