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The map label for this gene is hypB [H]

Identifier: 148380173

GI number: 148380173

Start: 2378523

End: 2379161

Strand: Direct

Name: hypB [H]

Synonym: CBO2220

Alternate gene names: 148380173

Gene position: 2378523-2379161 (Clockwise)

Preceding gene: 148380172

Following gene: 148380188

Centisome position: 61.19

GC content: 28.33

Gene sequence:

>639_bases
ATGAAAACCATAGAAATAAATGAAAGTGTTTATAAGAAAAATAGATCCATTGCCCACCATTTAAAAGATAAACTTCATGA
AAAAAACATAAAAATGATTAATTTATTAGGTTCACCAGGCTCCGGGAAAACCACTTTACTAGAAGCTATAATAAAAAATA
TTAATAATAGAGAAAAATTAGCGGTAATTGAAGGGGACTTATACACAGATAAAGATGCCAAACGAATTGAAAAATTAGGT
ATTAAAACTGTTCAACTTAATACTAAAGGAGCCTGCCACCTTGAAGCCGATGCTATAGTAGAAGCAGTAAATAACCTTAA
TATTAATAAGGAAGAACTTATAATTATTGATAATATAGGCAATTTAGTTTGTACCGCAGAATTTGACCTAGGAGAAGATA
TGAGAATAGCTGTTATGAGCGTTACTGAAGGTAACGATAAACCTTTAAAATATCCACTTATGTTTCAAACTACTAATGTA
ATTGCACTAAATAAAATTGATATATTACCTTATACTAATTTTGATTTAGAGCAATTTTATAAAGATGCTAAATGTCTAAA
TCCTAAGGTGAAAATATTTGAAGTATCAGCTACTAGAGGTGATGGAATAAATAAAATTTGTAATTTAATAGGTGGTTAA

Upstream 100 bases:

>100_bases
TATCCTTTACTCATAAAGAATGTCCAATTTGTCATAAAGTCAGTGAAAAAATTGTATCTGGGTATGATACCTATGTATAC
AGTATAGAAGGTGATTAAAA

Downstream 100 bases:

>100_bases
TTAAAACTAACCACCCATGTACTTATTCGTTGCAGTGTCCTTCATGTTGATGTTCTCTACATACACTACCTGTTGACTTT
AGTTCACCTTGTATATATTT

Product: hydrogenase isoenzyme nickel incorporation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKTIEINESVYKKNRSIAHHLKDKLHEKNIKMINLLGSPGSGKTTLLEAIIKNINNREKLAVIEGDLYTDKDAKRIEKLG
IKTVQLNTKGACHLEADAIVEAVNNLNINKEELIIIDNIGNLVCTAEFDLGEDMRIAVMSVTEGNDKPLKYPLMFQTTNV
IALNKIDILPYTNFDLEQFYKDAKCLNPKVKIFEVSATRGDGINKICNLIGG

Sequences:

>Translated_212_residues
MKTIEINESVYKKNRSIAHHLKDKLHEKNIKMINLLGSPGSGKTTLLEAIIKNINNREKLAVIEGDLYTDKDAKRIEKLG
IKTVQLNTKGACHLEADAIVEAVNNLNINKEELIIIDNIGNLVCTAEFDLGEDMRIAVMSVTEGNDKPLKYPLMFQTTNV
IALNKIDILPYTNFDLEQFYKDAKCLNPKVKIFEVSATRGDGINKICNLIGG
>Mature_212_residues
MKTIEINESVYKKNRSIAHHLKDKLHEKNIKMINLLGSPGSGKTTLLEAIIKNINNREKLAVIEGDLYTDKDAKRIEKLG
IKTVQLNTKGACHLEADAIVEAVNNLNINKEELIIIDNIGNLVCTAEFDLGEDMRIAVMSVTEGNDKPLKYPLMFQTTNV
IALNKIDILPYTNFDLEQFYKDAKCLNPKVKIFEVSATRGDGINKICNLIGG

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=209, Percent_Identity=42.5837320574163, Blast_Score=184, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 23683; Mature: 23683

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEINESVYKKNRSIAHHLKDKLHEKNIKMINLLGSPGSGKTTLLEAIIKNINNREKL
CCEEECCHHHHHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEE
AVIEGDLYTDKDAKRIEKLGIKTVQLNTKGACHLEADAIVEAVNNLNINKEELIIIDNIG
EEEECCCCCCHHHHHHHHCCCEEEEECCCCCEEECHHHHHHHHHHCCCCCCEEEEEECCC
NLVCTAEFDLGEDMRIAVMSVTEGNDKPLKYPLMFQTTNVIALNKIDILPYTNFDLEQFY
CEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEEECCEEEEEEEEEEECCCCCHHHHH
KDAKCLNPKVKIFEVSATRGDGINKICNLIGG
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKTIEINESVYKKNRSIAHHLKDKLHEKNIKMINLLGSPGSGKTTLLEAIIKNINNREKL
CCEEECCHHHHHHCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEE
AVIEGDLYTDKDAKRIEKLGIKTVQLNTKGACHLEADAIVEAVNNLNINKEELIIIDNIG
EEEECCCCCCHHHHHHHHCCCEEEEECCCCCEEECHHHHHHHHHHCCCCCCEEEEEECCC
NLVCTAEFDLGEDMRIAVMSVTEGNDKPLKYPLMFQTTNVIALNKIDILPYTNFDLEQFY
CEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEEEEECCEEEEEEEEEEECCCCCHHHHH
KDAKCLNPKVKIFEVSATRGDGINKICNLIGG
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]