| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is hopD2 [H]
Identifier: 148380169
GI number: 148380169
Start: 2371505
End: 2373967
Strand: Direct
Name: hopD2 [H]
Synonym: CBO2216
Alternate gene names: 148380169
Gene position: 2371505-2373967 (Clockwise)
Preceding gene: 148380168
Following gene: 148380172
Centisome position: 61.01
GC content: 28.79
Gene sequence:
>2463_bases TTGAAAAAAAATTTTAAATTAAATATATTTATTCTATTAATATTAGTAGGTGTATTTTCCTTTTTCTCAATAACAAATAA AGCTACTATAGCTAAAGATGATAATAATGGTGTACATCTAGTTTTAGATTCACGAAATAACAATAAGGTTCCAAAAAAAT TTCGTAAATCCTCTGATATATCAAATGTAGAGAAAGATAAAAATGTGAATCTTACTGGATTGAACACATTAAATATTTCT GGTAGTAAGCAATTTTCTAAGCAAAATCTTCCCCTTATAATAAATAATATAGGAACTTCACTACCTATAACAGTTGTTAA TTTAAGACAGGAATCCCATGGATTTATTAATGGATTACCCGTTAGTTGGGCTAATAAAAAAAATAATGCTAATGCAGGTT TAACAAAAGCTGAAGTTTTAAAAGATGAAAATAATAAATTAGAAAGTATTAAATTGAATTCACCCATTAGTTTTTACAAT CATCCTGATAAAACTATAATTCCAACAAAAGTTGAAAACGAAGAACAGCTTGTTAAGCATAATTCATTATCATATGTCCG CATACCTGTTACCGATACCAAGTTACCTACAGATGATATGGTTGATTATTTTGTCGATGTGATTAAATCTAACCCTAAGG ACACATGGTACCATTTTCACTGTAAGCAAGGCATAGGAAGAACTACTACTTTTATGATTATGTATGATATGATGAGAAAT GCAAAAGAAGTTCCTGCTGATGACATTATAAAAAGACAATTATTATTAGCAGACTTTGATGAAAAGCATATGAAATCTTT TTATAATAACGAAAGACATGATTTCTTACAAAATTTCTATAAATATGCTAAGGAAAATGGTAGTAATTTCGATGTAAAAT GGAGCGATTGGAAGAAAACTCTTAACACTAAAAGCAATAGTTTTTTTCCTATTGCTTCTTCAAATAAAGAATCTTCTAAT TATATAAAAAACCCTAAAATTCCAACCCATCTATATGTTATTTCTCAAAATGAAATGACTTCTAGCGAAAGAACAATGAT AGCTACTCTTCAAGGGATAGTTAATAATCATTGTTCTCATCAAATATATACCCTTAATTCTTCACAACCAGATTACCAAA CATGGCTAAATGATTTAAAAAATAATTATGGTGTATCTTACAATATTGTTTCAGACCCCTGGGAGTTATTAAATATATAT AAGGACTATATAAAAGGCTATGTACTTTATAGCAACAAATCATCAAAAGATCCTTCTATAAATAATGCTTGTTCCCTTGC TTCATTAAAAAATTCCATTGCAATTGATGAAATCATTGAAAATAAAGTACGAGCCCACAGCATAACAAATATTAGTGGAG ATTGTAGAAATACAGATAAAGATTGGGCCTATAATAATTTGTGGAATTCTGGATTAAATCATTCAATAGTAATTCAATTA TCTCCTAAAAAAGAAACAGCTTTAAGAGACTACGCTATAATGACTAAGTCTCTAATATTTTATGAAGATAGCATAAATGA TACATCCCTTAGAGATAAAGTATTCTCCTCTATGGATCCTAATTCTATTTGTTTAGGTTGGGGACCAGATGAATTTATAA ATGTAAGCACTTCATCAAAACATGGTGTAAGTATGATAGCCGCAGATTGGTCTTATAATTTAACTGTATTGAGCGCCTTT CCCTCATCCCCTATGGCTCAAAAATCCTCATCAAATATAACTAATAAAAAAAATGTTCATTATGTAACATTTATTATGTC AGATGGAGATAACCAGCAATGGAATCTTGGAACTAATTATGGTTCTCCTAAGTGGTATGGTTCTCCTTATAGGGGTAACT TTAATCTTGGTTGGTCTTTAAGTCCATCTTTATATTATTTAGCTCCTACTGTTTTTAATTTATATTATAAAAGCGCCTCT CATGGATCTACTAATGATTATTTTATAGTATCACCCTCAGGCAATGGGTATATGTATCCTAGCAAATATGATAAGAATGT CCTAGGCGCATATATTAATACATTAGATGACTATATGAAAAAAGCAGATGAGAAATATGTAGCAATTATAGATGACTCTT CTTTTTATAATAATAAACTTTGGGATAAATTTACTGTTAAGCCTAATATACAAGGATTGTTTTATCTTGATTACCGAAAG CATAACAACTATCATGGAGAAATTATTTGGTCTAATAATAAACCTATAGTATCTTGTAGGGACCTACTTTGGAATAATCT AGAGAGCGAAGATGAATTGGTTAAAAATATTAATAAAAGAATTAACTCTGGTGAGAAAGACATTCATAATCCTAATAGCT ACACCTTTGTATATGTTCATGTTTGGAGCAAAAACTTAAATAATATTGAAGATACTGTTAATAAGCTCAAGAAAAATCCT AAAGTAGAAATAGTAACTCCTGAAATATTTATGGAATTGATAAATAAAAATGTTAAACATTGA
Upstream 100 bases:
>100_bases GTATACAAGTTATATAAATAACACATTCTATATTTATGTTAAGTAATTGTTAATTAAAGATACAATTTACTTAAAATTAA TATAAAAGAGGTGATTTGGA
Downstream 100 bases:
>100_bases TTCTTAAAAATATTTAACCTACTATAAACAAATTTTAGTAGGTTAAATATGTGATCTAAAAATAATTAATTTATAAAAGT GATTTATCTGGAGTTACAAT
Product: exported protein
Products: NA
Alternate protein names: Tyrosine-protein phosphatase hopPtoD2 [H]
Number of amino acids: Translated: 820; Mature: 820
Protein sequence:
>820_residues MKKNFKLNIFILLILVGVFSFFSITNKATIAKDDNNGVHLVLDSRNNNKVPKKFRKSSDISNVEKDKNVNLTGLNTLNIS GSKQFSKQNLPLIINNIGTSLPITVVNLRQESHGFINGLPVSWANKKNNANAGLTKAEVLKDENNKLESIKLNSPISFYN HPDKTIIPTKVENEEQLVKHNSLSYVRIPVTDTKLPTDDMVDYFVDVIKSNPKDTWYHFHCKQGIGRTTTFMIMYDMMRN AKEVPADDIIKRQLLLADFDEKHMKSFYNNERHDFLQNFYKYAKENGSNFDVKWSDWKKTLNTKSNSFFPIASSNKESSN YIKNPKIPTHLYVISQNEMTSSERTMIATLQGIVNNHCSHQIYTLNSSQPDYQTWLNDLKNNYGVSYNIVSDPWELLNIY KDYIKGYVLYSNKSSKDPSINNACSLASLKNSIAIDEIIENKVRAHSITNISGDCRNTDKDWAYNNLWNSGLNHSIVIQL SPKKETALRDYAIMTKSLIFYEDSINDTSLRDKVFSSMDPNSICLGWGPDEFINVSTSSKHGVSMIAADWSYNLTVLSAF PSSPMAQKSSSNITNKKNVHYVTFIMSDGDNQQWNLGTNYGSPKWYGSPYRGNFNLGWSLSPSLYYLAPTVFNLYYKSAS HGSTNDYFIVSPSGNGYMYPSKYDKNVLGAYINTLDDYMKKADEKYVAIIDDSSFYNNKLWDKFTVKPNIQGLFYLDYRK HNNYHGEIIWSNNKPIVSCRDLLWNNLESEDELVKNINKRINSGEKDIHNPNSYTFVYVHVWSKNLNNIEDTVNKLKKNP KVEIVTPEIFMELINKNVKH
Sequences:
>Translated_820_residues MKKNFKLNIFILLILVGVFSFFSITNKATIAKDDNNGVHLVLDSRNNNKVPKKFRKSSDISNVEKDKNVNLTGLNTLNIS GSKQFSKQNLPLIINNIGTSLPITVVNLRQESHGFINGLPVSWANKKNNANAGLTKAEVLKDENNKLESIKLNSPISFYN HPDKTIIPTKVENEEQLVKHNSLSYVRIPVTDTKLPTDDMVDYFVDVIKSNPKDTWYHFHCKQGIGRTTTFMIMYDMMRN AKEVPADDIIKRQLLLADFDEKHMKSFYNNERHDFLQNFYKYAKENGSNFDVKWSDWKKTLNTKSNSFFPIASSNKESSN YIKNPKIPTHLYVISQNEMTSSERTMIATLQGIVNNHCSHQIYTLNSSQPDYQTWLNDLKNNYGVSYNIVSDPWELLNIY KDYIKGYVLYSNKSSKDPSINNACSLASLKNSIAIDEIIENKVRAHSITNISGDCRNTDKDWAYNNLWNSGLNHSIVIQL SPKKETALRDYAIMTKSLIFYEDSINDTSLRDKVFSSMDPNSICLGWGPDEFINVSTSSKHGVSMIAADWSYNLTVLSAF PSSPMAQKSSSNITNKKNVHYVTFIMSDGDNQQWNLGTNYGSPKWYGSPYRGNFNLGWSLSPSLYYLAPTVFNLYYKSAS HGSTNDYFIVSPSGNGYMYPSKYDKNVLGAYINTLDDYMKKADEKYVAIIDDSSFYNNKLWDKFTVKPNIQGLFYLDYRK HNNYHGEIIWSNNKPIVSCRDLLWNNLESEDELVKNINKRINSGEKDIHNPNSYTFVYVHVWSKNLNNIEDTVNKLKKNP KVEIVTPEIFMELINKNVKH >Mature_820_residues MKKNFKLNIFILLILVGVFSFFSITNKATIAKDDNNGVHLVLDSRNNNKVPKKFRKSSDISNVEKDKNVNLTGLNTLNIS GSKQFSKQNLPLIINNIGTSLPITVVNLRQESHGFINGLPVSWANKKNNANAGLTKAEVLKDENNKLESIKLNSPISFYN HPDKTIIPTKVENEEQLVKHNSLSYVRIPVTDTKLPTDDMVDYFVDVIKSNPKDTWYHFHCKQGIGRTTTFMIMYDMMRN AKEVPADDIIKRQLLLADFDEKHMKSFYNNERHDFLQNFYKYAKENGSNFDVKWSDWKKTLNTKSNSFFPIASSNKESSN YIKNPKIPTHLYVISQNEMTSSERTMIATLQGIVNNHCSHQIYTLNSSQPDYQTWLNDLKNNYGVSYNIVSDPWELLNIY KDYIKGYVLYSNKSSKDPSINNACSLASLKNSIAIDEIIENKVRAHSITNISGDCRNTDKDWAYNNLWNSGLNHSIVIQL SPKKETALRDYAIMTKSLIFYEDSINDTSLRDKVFSSMDPNSICLGWGPDEFINVSTSSKHGVSMIAADWSYNLTVLSAF PSSPMAQKSSSNITNKKNVHYVTFIMSDGDNQQWNLGTNYGSPKWYGSPYRGNFNLGWSLSPSLYYLAPTVFNLYYKSAS HGSTNDYFIVSPSGNGYMYPSKYDKNVLGAYINTLDDYMKKADEKYVAIIDDSSFYNNKLWDKFTVKPNIQGLFYLDYRK HNNYHGEIIWSNNKPIVSCRDLLWNNLESEDELVKNINKRINSGEKDIHNPNSYTFVYVHVWSKNLNNIEDTVNKLKKNP KVEIVTPEIFMELINKNVKH
Specific function: Effector showing tyrosine-phosphatase activity required for host defense suppression. Functions inside plant cells causing suppression of HR (hypersensitive response), PR1 gene expression and oxidative burst probably by interfering with a MAPK (mitogen- a
COG id: NA
COG function: NA
Gene ontology:
Cell location: Secreted. Note=Secreted via type III secretion system (TTSS) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 tyrosine-protein phosphatase domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000387 - InterPro: IPR016130 - InterPro: IPR000242 [H]
Pfam domain/function: PF00102 Y_phosphatase [H]
EC number: =3.1.3.48 [H]
Molecular weight: Translated: 94023; Mature: 94023
Theoretical pI: Translated: 9.11; Mature: 9.11
Prosite motif: PS00383 TYR_PHOSPHATASE_1 ; PS50056 TYR_PHOSPHATASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKNFKLNIFILLILVGVFSFFSITNKATIAKDDNNGVHLVLDSRNNNKVPKKFRKSSDI CCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHCCCC SNVEKDKNVNLTGLNTLNISGSKQFSKQNLPLIINNIGTSLPITVVNLRQESHGFINGLP CCCCCCCCCEEEEEEEEECCCCCHHCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCC VSWANKKNNANAGLTKAEVLKDENNKLESIKLNSPISFYNHPDKTIIPTKVENEEQLVKH CEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCEEECCCCCCEECCCCCCCHHHHHHC NSLSYVRIPVTDTKLPTDDMVDYFVDVIKSNPKDTWYHFHCKQGIGRTTTFMIMYDMMRN CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHC AKEVPADDIIKRQLLLADFDEKHMKSFYNNERHDFLQNFYKYAKENGSNFDVKWSDWKKT HHCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH LNTKSNSFFPIASSNKESSNYIKNPKIPTHLYVISQNEMTSSERTMIATLQGIVNNHCSH HCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC QIYTLNSSQPDYQTWLNDLKNNYGVSYNIVSDPWELLNIYKDYIKGYVLYSNKSSKDPSI EEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCEEEEECCCCCCCCCC NNACSLASLKNSIAIDEIIENKVRAHSITNISGDCRNTDKDWAYNNLWNSGLNHSIVIQL CCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEE SPKKETALRDYAIMTKSLIFYEDSINDTSLRDKVFSSMDPNSICLGWGPDEFINVSTSSK CCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEECCCCC HGVSMIAADWSYNLTVLSAFPSSPMAQKSSSNITNKKNVHYVTFIMSDGDNQQWNLGTNY CCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCC GSPKWYGSPYRGNFNLGWSLSPSLYYLAPTVFNLYYKSASHGSTNDYFIVSPSGNGYMYP CCCCCCCCCCCCCEEECEEECCCEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECC SKYDKNVLGAYINTLDDYMKKADEKYVAIIDDSSFYNNKLWDKFTVKPNIQGLFYLDYRK CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEEEEEC HNNYHGEIIWSNNKPIVSCRDLLWNNLESEDELVKNINKRINSGEKDIHNPNSYTFVYVH CCCCEEEEEECCCCCEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE VWSKNLNNIEDTVNKLKKNPKVEIVTPEIFMELINKNVKH EECCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCC >Mature Secondary Structure MKKNFKLNIFILLILVGVFSFFSITNKATIAKDDNNGVHLVLDSRNNNKVPKKFRKSSDI CCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHCCCC SNVEKDKNVNLTGLNTLNISGSKQFSKQNLPLIINNIGTSLPITVVNLRQESHGFINGLP CCCCCCCCCEEEEEEEEECCCCCHHCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCC VSWANKKNNANAGLTKAEVLKDENNKLESIKLNSPISFYNHPDKTIIPTKVENEEQLVKH CEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCEEECCCCCCEECCCCCCCHHHHHHC NSLSYVRIPVTDTKLPTDDMVDYFVDVIKSNPKDTWYHFHCKQGIGRTTTFMIMYDMMRN CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHC AKEVPADDIIKRQLLLADFDEKHMKSFYNNERHDFLQNFYKYAKENGSNFDVKWSDWKKT HHCCCHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH LNTKSNSFFPIASSNKESSNYIKNPKIPTHLYVISQNEMTSSERTMIATLQGIVNNHCSH HCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC QIYTLNSSQPDYQTWLNDLKNNYGVSYNIVSDPWELLNIYKDYIKGYVLYSNKSSKDPSI EEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCEEEEECCCCCCCCCC NNACSLASLKNSIAIDEIIENKVRAHSITNISGDCRNTDKDWAYNNLWNSGLNHSIVIQL CCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEE SPKKETALRDYAIMTKSLIFYEDSINDTSLRDKVFSSMDPNSICLGWGPDEFINVSTSSK CCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEECCCCC HGVSMIAADWSYNLTVLSAFPSSPMAQKSSSNITNKKNVHYVTFIMSDGDNQQWNLGTNY CCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCC GSPKWYGSPYRGNFNLGWSLSPSLYYLAPTVFNLYYKSASHGSTNDYFIVSPSGNGYMYP CCCCCCCCCCCCCEEECEEECCCEEEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECC SKYDKNVLGAYINTLDDYMKKADEKYVAIIDDSSFYNNKLWDKFTVKPNIQGLFYLDYRK CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEEEEEC HNNYHGEIIWSNNKPIVSCRDLLWNNLESEDELVKNINKRINSGEKDIHNPNSYTFVYVH CCCCEEEEEECCCCCEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE VWSKNLNNIEDTVNKLKKNPKVEIVTPEIFMELINKNVKH EECCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12437299; 12828636; 12928499 [H]