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The map label for this gene is rnfE [H]

Identifier: 148378374

GI number: 148378374

Start: 431960

End: 432559

Strand: Direct

Name: rnfE [H]

Synonym: CBO0371

Alternate gene names: 148378374

Gene position: 431960-432559 (Clockwise)

Preceding gene: 148378373

Following gene: 148378375

Centisome position: 11.11

GC content: 31.83

Gene sequence:

>600_bases
ATGAGCGTTTTAGGAGAAAGATTAAAAAATGGAATTATAGACGAAAATGTTACCTTTGTCCAAGTTTTAGCAATGTGTCC
TACTTTGGCAGTAACATCAAGTGCTGAGAATGGTATAGGTATGGGTATTGCATCTACAGTAGTTCTTATGGGTTCAAACT
TAGTTATATCATTACTTAGAAAAGTAATACCAGATAAGATTCGTATACCAGCATTTATAGTAGTAATAGCAGGATTTGTT
ACATTGCTTCAATTTTTAATGCAAGGTTTTGTACCAGATTTATATACAAAATTAGGTATATTTATACCACTTATAGTTGT
TAACTGTGTTATCCTAGGAAGAGCGGAAGCTTATGCATCAAAATATGGTCCAGTATCTTCAATATTTGATGGATTAGGTC
AAGGTCTAGGATTTACATTATCATTAGGAGTAATAGGAATGATAAGAGAATTATTAGGAGCAGGCCAATTATTTGGACAT
CAAATAATGCCATCTTCATTCCAACCAGCTTTAATAATGATACTTGCGCCAGGAGCATTCTTTACACTAGGAATATTAAT
GGCAATAATAAACAATATGAAATTAAAGAAAGCTAAATAA

Upstream 100 bases:

>100_bases
GATGGTAAATCTGGAGCTACAACAAGCTTTAACACAGAGGACACAAAGTTAAATAAGTATGTTGTAAACTATGAAAAAAT
GAATTAAAGGGGGATAAATG

Downstream 100 bases:

>100_bases
CCAAGAGAAGGAATTTAAAATATCCTTATTAAGAAAGAAGGGTGAAAAGTATGAAAATATTTGCTTTGATTATATCAGCG
TTATTTGTTAATAACTTCGT

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfE [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLRKVIPDKIRIPAFIVVIAGFV
TLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYASKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGH
QIMPSSFQPALIMILAPGAFFTLGILMAIINNMKLKKAK

Sequences:

>Translated_199_residues
MSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLRKVIPDKIRIPAFIVVIAGFV
TLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYASKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGH
QIMPSSFQPALIMILAPGAFFTLGILMAIINNMKLKKAK
>Mature_198_residues
SVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLRKVIPDKIRIPAFIVVIAGFVT
LLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYASKYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGHQ
IMPSSFQPALIMILAPGAFFTLGILMAIINNMKLKKAK

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=201, Percent_Identity=42.7860696517413, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1787914, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 21157; Mature: 21026

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLR
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHH
KVIPDKIRIPAFIVVIAGFVTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYAS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGHQIMPSSFQPALIMILAPGAF
HCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHH
FTLGILMAIINNMKLKKAK
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVLGERLKNGIIDENVTFVQVLAMCPTLAVTSSAENGIGMGIASTVVLMGSNLVISLLR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHH
KVIPDKIRIPAFIVVIAGFVTLLQFLMQGFVPDLYTKLGIFIPLIVVNCVILGRAEAYAS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYGPVSSIFDGLGQGLGFTLSLGVIGMIRELLGAGQLFGHQIMPSSFQPALIMILAPGAF
HCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHH
FTLGILMAIINNMKLKKAK
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268 [H]