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The map label for this gene is vanS [H]

Identifier: 148378369

GI number: 148378369

Start: 424776

End: 426254

Strand: Direct

Name: vanS [H]

Synonym: CBO0366

Alternate gene names: 148378369

Gene position: 424776-426254 (Clockwise)

Preceding gene: 148378368

Following gene: 148378370

Centisome position: 10.93

GC content: 23.94

Gene sequence:

>1479_bases
ATGAAACCTTTCAAATATATATATAAATTAATATCTTATAAAGTAAAAAAAAGTTTAAGATTAGAACTTATATTTACTTT
TGGATTATGTTTTTTAATGGCTATTATGACCTTTATTATAACTAATAGTTACCTAACTAAAGCTAGTAAATATTCAGAAA
TAAATTATGAAAAAGGAATAAATGAAATTTACAATTATTCTTTATCTATATCAAATGAGATTAATAGTGAAAAATTAAAA
ATAGAAGATAAAGACACTATACAAGAACTTATAGATAGACATTGGAGCATGGATAAAAATAATAAAATATTTATAGCTAA
TACTGATGGCAAAATTCTTTTTAAAGTAGAAAATGTAGAAGTTGAAAAAGTTGATATATTTGAAATTATAAAAAATAATA
TACAACTTCAAAATATAAAGAGTTATGAGTATGATTATGATGTAGGAAGAAAAGAATTTGTAAGTATCAATCCATTAATT
TTTGAAAATGGAAAAGCTTATGTAATAGTTCAAGGCATACCAAGAGCAGAAACCATATATTATACAAAGGATAAATCTGT
GAGTGCATTTTTATTAGCTGTTATAGTATTTATATTTGGATTTTTATTTATAACAAAAAGGAAGATGAATTATATAGAAG
AAATATCAAAGGGCTTGCTGCAAATATCAAAAGGAAATTTAGATTATAGAGTAAAAAGAGTAGGAAATGATGAGTTAGCA
CTACTAGCAGATAATATAAATTATATGGCAAAAGAGTTAACTGATAAAATAGAAAAAGAAAGAAAAATAGAAAAAGCTAA
AAATGACTTAATAACAAATGTTTCCCATGATTTAAGAACTCCACTTACATCTATAATGGGGTATTTAGGATTAATAAAAG
AAAAAAAATTTAAAAGTGAAAAAGAATTAATGGAATATGCAAGTGTAGCTTACACTAAATCGGAAAAACTAAAAAATTTA
ATACAGGATTTATTTTCTTATACTAAATATAGTAATGACAGAGTAGAATTACACAAGCAGAAGATTATTTTAGCAGAATT
ATTAGATCAATTAATAGAAGAATTAGTACCTATATGTGAAGAAAATAAAGTAAGCATAAATAAATATGCTTGGGATTCTG
AAATAGTATCAGAAATAGATGCTGACAAAATGGTAAGAGTTTTTGAAAATCTAATAATGAATGCTATAAGATATAGTATA
AAGCCAGGAGAAATAAAAATTAATTTGTACAAAGAAAGAGTATTTTCTATGGTATCCATATCAAATAAAAGTGAAGAAAT
ATCAAAAGATAACTTAAGAAAATTATTTGATAGATTTTATAGATTAGATAAATCAAGAACTGCATCAACCGGTGGAAGTG
GCTTAGGATTAGCTATAGCCAAGAGTATAGTAGAATTGCATAAAGGTAGTATTTGGGCGGAATATCAAAAGGGATATATA
ACTTTTTATATAAAATTAATTATGGCAGAGGATGAATAA

Upstream 100 bases:

>100_bases
AAAATAGAACAAAATCCTAGAAATCCAAGATATATAAAAACAGTTTGGGGAGTTGGATATAAGGTTGAAAAATAGAATAA
AGAGATTATTTAATAAAATA

Downstream 100 bases:

>100_bases
AATTTATTAAAACCTGGTGCAATTGCATCAGGTTTTAAGTTATAATATAATGAAAAATATTATATATAAAATATTTATTA
AACAAACATATTTAAGAGGA

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGINEIYNYSLSISNEINSEKLK
IEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLI
FENGKAYVIVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLDYRVKRVGNDELA
LLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSEKELMEYASVAYTKSEKLKNL
IQDLFSYTKYSNDRVELHKQKIILAELLDQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSI
KPGEIKINLYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHKGSIWAEYQKGYI
TFYIKLIMAEDE

Sequences:

>Translated_492_residues
MKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGINEIYNYSLSISNEINSEKLK
IEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLI
FENGKAYVIVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLDYRVKRVGNDELA
LLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSEKELMEYASVAYTKSEKLKNL
IQDLFSYTKYSNDRVELHKQKIILAELLDQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSI
KPGEIKINLYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHKGSIWAEYQKGYI
TFYIKLIMAEDE
>Mature_492_residues
MKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGINEIYNYSLSISNEINSEKLK
IEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVEVEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLI
FENGKAYVIVQGIPRAETIYYTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLDYRVKRVGNDELA
LLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSEKELMEYASVAYTKSEKLKNL
IQDLFSYTKYSNDRVELHKQKIILAELLDQLIEELVPICEENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSI
KPGEIKINLYKERVFSMVSISNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHKGSIWAEYQKGYI
TFYIKLIMAEDE

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=28.0701754385965, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786783, Length=298, Percent_Identity=25.8389261744966, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786600, Length=247, Percent_Identity=25.5060728744939, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787894, Length=325, Percent_Identity=24.9230769230769, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790346, Length=259, Percent_Identity=25.8687258687259, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788713, Length=243, Percent_Identity=24.2798353909465, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=26.7206477732794, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=25.5905511811024, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI145693157, Length=278, Percent_Identity=21.2230215827338, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=24.7787610619469, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87082128, Length=327, Percent_Identity=23.5474006116208, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994928, Length=249, Percent_Identity=22.4899598393574, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789149, Length=335, Percent_Identity=23.5820895522388, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787374, Length=406, Percent_Identity=23.8916256157635, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57330; Mature: 57330

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCH
NEIYNYSLSISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVE
HHHHCCEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCE
VEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLIFENGKAYVIVQGIPRAETIY
EEHHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEEECCEEEECCCEEEEEECCCCCCEEE
YTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLDYRVKRVGNDELA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEE
LLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSE
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCH
KELMEYASVAYTKSEKLKNLIQDLFSYTKYSNDRVELHKQKIILAELLDQLIEELVPICE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSIKPGEIKINLYKERVFSMVSI
CCCCEEEHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHC
SNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHKGSIWAEYQKGYI
CCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHCCEE
TFYIKLIMAEDE
EEEEEEEEECCC
>Mature Secondary Structure
MKPFKYIYKLISYKVKKSLRLELIFTFGLCFLMAIMTFIITNSYLTKASKYSEINYEKGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCH
NEIYNYSLSISNEINSEKLKIEDKDTIQELIDRHWSMDKNNKIFIANTDGKILFKVENVE
HHHHCCEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEECCE
VEKVDIFEIIKNNIQLQNIKSYEYDYDVGRKEFVSINPLIFENGKAYVIVQGIPRAETIY
EEHHHHHHHHHCCCEEEECCCCCCCCCCCCHHEEEECCEEEECCCEEEEEECCCCCCEEE
YTKDKSVSAFLLAVIVFIFGFLFITKRKMNYIEEISKGLLQISKGNLDYRVKRVGNDELA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCEE
LLADNINYMAKELTDKIEKERKIEKAKNDLITNVSHDLRTPLTSIMGYLGLIKEKKFKSE
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCH
KELMEYASVAYTKSEKLKNLIQDLFSYTKYSNDRVELHKQKIILAELLDQLIEELVPICE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ENKVSINKYAWDSEIVSEIDADKMVRVFENLIMNAIRYSIKPGEIKINLYKERVFSMVSI
CCCCEEEHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHC
SNKSEEISKDNLRKLFDRFYRLDKSRTASTGGSGLGLAIAKSIVELHKGSIWAEYQKGYI
CCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHCCEE
TFYIKLIMAEDE
EEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]