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The map label for this gene is ksgA [H]

Identifier: 148378088

GI number: 148378088

Start: 111827

End: 112663

Strand: Direct

Name: ksgA [H]

Synonym: CBO0081

Alternate gene names: 148378088

Gene position: 111827-112663 (Clockwise)

Preceding gene: 148378087

Following gene: 148378089

Centisome position: 2.88

GC content: 27.36

Gene sequence:

>837_bases
ATGAATAATATGAATACAAAGGAAATAGTTAACAAATATGAATTTAAGTTTAATAAGAATTTAGGACAAAACTTTTTAAT
AGATGAATCTGTATTGGAAGATATAATAGAGGGAGCTGAAATAAACAAAGAAGATACTGTTATAGAGATAGGCCCTGGGG
TTGGGACTTTAACTAAGGAGCTTTTAGAAAGAGCAAAAGAAGTTTACTCTATAGAATTAGATGGTGATTTAATACCTATT
TTGCAAGAGGAGTTAAAGGAATATAATAATTTTACACTAATACATAAAGATGCGTTAAAAATTGATTTTAATGAGTTAAT
GGAAAATAAGGATTCCATAAAATTAGTAGCCAATCTACCTTATTATGTAACTACACCTATTATATCAAGATTACTTACAG
AAAAATGTGATTTTAAATCACTAACCATAATGATTCAAAAGGAAGTAGCGGAAAGAATAAATGCTGAACCTAACTGTAAG
GAGTATGGTTCATTAACCGTTTTAGTACAATATTATTGTAATACAAAAATAATAAGAAAAGTATCTCCAAATTCTTTTAT
ACCAAGACCTAAAGTGGATTCTATAGTTATAAAATTAGATAGACTTTCAGAACCAAGAGTAAGGGTGAAAAGCCAAAAAT
TATTTTTTAATGTAGTAAGAAGTTCATTTAATATGAGAAGAAAAACTTTATGGAATTCATTAAAAAGTTTAAATATAGAT
AAAGAAAGTATGGAAAATGCTTTTGAAAGAGCAGGGATAGATCCAAAACGAAGGGGCGAGACTTTATCCATAGAAGAATT
CGGAAAACTTTCAGATTGTATATATGATATATTATAA

Upstream 100 bases:

>100_bases
ATAGGCTATTGCAATACTAATCAGTTTATAAAAAGACTTAACAATTTTGGTATAACCAAAGAAGAGTTTATAAAGGCTAT
AAAAGATTTAGATAAGGGGA

Downstream 100 bases:

>100_bases
TTTAAATAATTAAGTTCACTTTTTGTAAGATAACTCATATATTATATTGAATAATTATATTTGGGGGTACAATAAAAAGT
GGCACAAAAAAAGAATTATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNNMNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPI
LQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCK
EYGSLTVLVQYYCNTKIIRKVSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNID
KESMENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL

Sequences:

>Translated_278_residues
MNNMNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPI
LQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCK
EYGSLTVLVQYYCNTKIIRKVSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNID
KESMENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
>Mature_278_residues
MNNMNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPI
LQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCK
EYGSLTVLVQYYCNTKIIRKVSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNID
KESMENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=288, Percent_Identity=30.5555555555556, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=32.7354260089686, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=35.7976653696498, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25141369, Length=293, Percent_Identity=26.962457337884, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=197, Percent_Identity=33.502538071066, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=227, Percent_Identity=31.7180616740088, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21358017, Length=201, Percent_Identity=34.3283582089552, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21357273, Length=284, Percent_Identity=28.5211267605634, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32173; Mature: 32173

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKE
CCCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHH
LLERAKEVYSIELDGDLIPILQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLP
HHHHHHHHEEEEECCCCHHHHHHHHHHHCCEEEEECCCCEECHHHHHCCCCCEEEEECCC
YYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYGSLTVLVQYYCNTKIIRK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEHHHCCCEEEE
VSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNID
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KESMENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
HHHHHHHHHHCCCCHHHCCCEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNMNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKE
CCCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHH
LLERAKEVYSIELDGDLIPILQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLP
HHHHHHHHEEEEECCCCHHHHHHHHHHHCCEEEEECCCCEECHHHHHCCCCCEEEEECCC
YYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYGSLTVLVQYYCNTKIIRK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEHHHCCCEEEE
VSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNID
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KESMENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
HHHHHHHHHHCCCCHHHCCCEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA