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The map label for this gene is recF [H]

Identifier: 148378015

GI number: 148378015

Start: 2998

End: 4101

Strand: Direct

Name: recF [H]

Synonym: CBO0004

Alternate gene names: 148378015

Gene position: 2998-4101 (Clockwise)

Preceding gene: 148378014

Following gene: 148378016

Centisome position: 0.08

GC content: 23.19

Gene sequence:

>1104_bases
GTGTTTAGAATGTATATAAAAAATGTGCACTTAATAAATTTTAGAAATTATGATGATATGTATTTAGAATTAAGTCCAAA
TACAAATATTTTTGTAGGTAATAATGCTCAAGGGAAAACTAATATACTAGAAAGTATATATTATTCAAGTATAGGAAAGT
CTCATAGAACAAATAAAGATAAAGATCTTATAAAATGGGATAAAAATAATACATACTTAAGGACATATGTATCCAGGGAA
AGATTAGATAAAACTATAGATATAAATATATTTAAAAATGGCAAAAAAGCTATAACAGTGAATAAAATAAAAATAAAAAA
AATATCTGAACTAATGGGGAACTTAAATGTTGTTATGTTTTCGCCAGAAGACTTAAGAATAATTAAGGATTCCCCTGGAA
ATAGAAGGAAATTTCTAGATATAGAATTATGTAAAATAAACAATGTTTATTATCATGATTTAGTCCAATATAATAAAATT
TTATCTGAAAGAAATACAGCTTTAAAAAATTGGAATAACAAAATTAATGATATAATTGATATTTATGATGAGCAACTTTC
TAAATATGGAGCTTTTATAATTAAAGAGAGAAATAAGTATTTGGATAAATTAAATATTATTGGCAAAAATATACATAAGA
AGATAACCAATGATTTAGAAGACATAAACTTTAGATATTTAACTAATATAAAAGATTTTGATAATGCAGAAAAAGAATTA
TTAATAGTTCTTAAGAAAAATCGAAAAAAAGATCTTGAGAGAAATTCAACCTCTATTGGTCCTCACCGAGATGATTTTGA
AGTAAGTATAAATAATATAGATACTAGAATTTTCGGATCACAAGGACAACAAAGAACAGCTGTATTAACTTTAAAGTTTG
CATCATTGGAAATAATAAAAAACATAATAGGGGAATATCCTGTGCTTTTATTAGATGATGTACTATCTGAATTAGATTCA
AATAGACAGAAGTTTGTATTGAATTCTATTGATAAAATACAAACTATAATAACTTGTACTGGTATAGAAGAAATAGACAA
ATACTTAGATAAGAAACAATCTCAATTATATTTAGTTAATAATGGTAAAATAAAAAGAGTTTAA

Upstream 100 bases:

>100_bases
ACTTAATAGAGTTTAATAATGAAACATATAAAATTATTTAAAATTTTATATAAATTTTAAATAATTGTTACTAAAAAATA
TTATGGTATAATTAATTTAG

Downstream 100 bases:

>100_bases
AGCTGAAAGGAGATTAATTTATGTTTCTTCACTTAGGAGAAAATGTAGTAGTACCAATAAAAGATGTTATAGGAATATTC
GACATGGAAACTTCTATGTA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MFRMYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRE
RLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKI
LSERNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKEL
LIVLKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDS
NRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV

Sequences:

>Translated_367_residues
MFRMYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRE
RLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKI
LSERNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKEL
LIVLKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDS
NRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV
>Mature_367_residues
MFRMYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRE
RLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKI
LSERNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKEL
LIVLKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDS
NRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=370, Percent_Identity=24.0540540540541, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 43076; Mature: 43076

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRMYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKD
CEEEEEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
KDLIKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMF
CCCEEECCCCCEEEEHHHHHHCCCEEEEEEEECCCEEEEEEEEEHHHHHHHHCCCEEEEE
SPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSERNTALKNWNNKINDIID
CCCCEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKEL
HHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCEEE
LIVLKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIK
EEEEECCCHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCEEEEEEEEHHHHHHHH
NIIGEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVN
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCEEEEEE
NGKIKRV
CCEEECC
>Mature Secondary Structure
MFRMYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKD
CEEEEEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCC
KDLIKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMF
CCCEEECCCCCEEEEHHHHHHCCCEEEEEEEECCCEEEEEEEEEHHHHHHHHCCCEEEEE
SPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSERNTALKNWNNKINDIID
CCCCEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
IYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKEL
HHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCEEE
LIVLKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIK
EEEEECCCHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCEEEEEEEEHHHHHHHH
NIIGEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVN
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCEEEEEE
NGKIKRV
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA