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The map label for this gene is lasB [H]

Identifier: 148361317

GI number: 148361317

Start: 3545750

End: 3547411

Strand: Reverse

Name: lasB [H]

Synonym: LPC_3292

Alternate gene names: 148361317

Gene position: 3547411-3545750 (Counterclockwise)

Preceding gene: 148361319

Following gene: 148361316

Centisome position: 99.19

GC content: 38.45

Gene sequence:

>1662_bases
ATGAAAAAACGGATTGTTTCATTATTTGTCATTTCAACAGTTATTTCATCTGTAAACGCTGCAACCCAAGCAATCGTATG
GGGTGAGTCAACGAAAGCATTGCCTCAATTTATGCAAGCACATCCTTTTAAGAAACAAGGATTATTGCAAACTGTAAAAA
GTCAGCAAAGTGATTACAAACTGGAGTTACAAAATAATTCTAACCATGCGACCAGGCATGCTCGCTATCAAATAACCTAC
AAGGGGGTTCCGGTTTGGGGTTATCAAGTCATTTTTCATTCCAAAGATGGCGCCAAAGAAACTGTAACAGGTATGAATAT
TACCGGAATTGAACAGGATATTAATTCTACTGAGGGAAAATTGAGCCCGAATGATATAGAACAAAAGATTCTTGGAAAGG
TAACTGAACCGGTTAAATTTAAAAACCTTAAAAAGGTTATTTATATAGATAAAGGAAATAAAGCTCATTTGGCTTATCAT
TTGTCTTACTATTCCAATAGTGAAAAAAAACATGTGAATGCACCTAATTATCTTATTGATGCCAATAGCGGTGAGATTTT
GAAACAATGGGATGAGGTGCGTCATGAAAGAATTGGTCAGGGATTAGGCGGTAATGCGTTTACTCTCCCATACCGTCAGG
GAATGTTTCAGCATGGTAATGCTTTGCCGGGGCTTCCCTCATTAGGGAAGTTTGACGTTAATGTTGAGGATGGCTTATGT
CGTGTTGAAAATGAATCCATAAAAGTAATGAATTTGGAAAATCATAATATAGGTTATGACTTCTTTCCAATTACCACATT
TGCAGAATCTGTACTGAATTTAAGTGCTTTTTCCTATCCATGCGATGAAACCAACTTGTTTTTAAACTATGCTGATGGCA
GAACAGGTCCTGTCAATTATGCTTTTTCTCCAGTTAACGATACGATGTATTTTGCCCAACAAACGTTAGATATGTATCAA
AAAGTTTATGGTGTTAATCGTCCAATAGGTGATGATTTACCTATACGGGCTTACACCCATCTTGGTGATATGGATAACGC
TTTTGCAGTACCAACCATCAGTCTTGATGGGGTAGTTCTTGCACATCAGCAAATTGTAATCGGAAATGGTGATGAATTTT
TAACAGCTCCAGCCCAGAGTGTGTTGGGACATGAATTATCACATAATTTTACTGCCCTGCATTCCGGATTGATGTATGAA
GGGCAATCTGGGGGGATCAATGAATCTTTCTCTGATATGGCGGCAATTGCATTGTTAGATTATCTTAGTAAAGATTATCC
ATGGTATTGGGATGGTGAGGATTGGACCATTGGGCGTGAAGCTGTAAAAAGTGGGCAACCTATTCGTTATTTGGATGATC
CAGCCAAGGATGGAATGTCTATAGGGCATGCTAGTGAATACACTGATGCGTTGGATGTGCATATAACGAGCGGTGTATTT
AATAAAGCATTTTATTTATTAGCACATAAACCAGGCTGGTCTATACAAAAAGCATTTCAGGTTATGGTTGACGCCAATAT
GAATTATTGGTCTCCTATTGCATACTATGATTTTGCTGCATGTGGCGTCATTCAGGCAACCATAGATAAGCATTGGGATA
AAACACCTGTTATCGAGGCATTTGCCGAGGTGGGAGTCGTTTGTCCGATGCATAAAAGCTAG

Upstream 100 bases:

>100_bases
ATATAAAAAAATAAAATTATGTCGTAATTTGTGTCTTCTGATTGTTTTTTTCTCGAATTTGTAGCAGCATGAAGTTTCCA
CAAAAAATCAGGATGATATT

Downstream 100 bases:

>100_bases
TTTTAAGTTGTTTGACTTCAGTTCAAGCTACTTGAAACAACGAGAGTTGCTAAGGAAGATTGATGATTTTCTATAGAACG
CCTACTGGATAAGATAATTA

Product: virulence metalloprotease

Products: NA

Alternate protein names: HA/protease; Vibriolysin [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKKRIVSLFVISTVISSVNAATQAIVWGESTKALPQFMQAHPFKKQGLLQTVKSQQSDYKLELQNNSNHATRHARYQITY
KGVPVWGYQVIFHSKDGAKETVTGMNITGIEQDINSTEGKLSPNDIEQKILGKVTEPVKFKNLKKVIYIDKGNKAHLAYH
LSYYSNSEKKHVNAPNYLIDANSGEILKQWDEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLC
RVENESIKVMNLENHNIGYDFFPITTFAESVLNLSAFSYPCDETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQ
KVYGVNRPIGDDLPIRAYTHLGDMDNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYE
GQSGGINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVF
NKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS

Sequences:

>Translated_553_residues
MKKRIVSLFVISTVISSVNAATQAIVWGESTKALPQFMQAHPFKKQGLLQTVKSQQSDYKLELQNNSNHATRHARYQITY
KGVPVWGYQVIFHSKDGAKETVTGMNITGIEQDINSTEGKLSPNDIEQKILGKVTEPVKFKNLKKVIYIDKGNKAHLAYH
LSYYSNSEKKHVNAPNYLIDANSGEILKQWDEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLC
RVENESIKVMNLENHNIGYDFFPITTFAESVLNLSAFSYPCDETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQ
KVYGVNRPIGDDLPIRAYTHLGDMDNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYE
GQSGGINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVF
NKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS
>Mature_553_residues
MKKRIVSLFVISTVISSVNAATQAIVWGESTKALPQFMQAHPFKKQGLLQTVKSQQSDYKLELQNNSNHATRHARYQITY
KGVPVWGYQVIFHSKDGAKETVTGMNITGIEQDINSTEGKLSPNDIEQKILGKVTEPVKFKNLKKVIYIDKGNKAHLAYH
LSYYSNSEKKHVNAPNYLIDANSGEILKQWDEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLC
RVENESIKVMNLENHNIGYDFFPITTFAESVLNLSAFSYPCDETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQ
KVYGVNRPIGDDLPIRAYTHLGDMDNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYE
GQSGGINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVF
NKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS

Specific function: May play a role in the pathogenesis of cholera. Hap nicks and activates the A subunit of cholera enterotoxin and related enterotoxins [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR007280
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C; PF04151 PPC [H]

EC number: NA

Molecular weight: Translated: 61686; Mature: 61686

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIVSLFVISTVISSVNAATQAIVWGESTKALPQFMQAHPFKKQGLLQTVKSQQSDYK
CCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEE
LELQNNSNHATRHARYQITYKGVPVWGYQVIFHSKDGAKETVTGMNITGIEQDINSTEGK
EEEECCCCCCEEEEEEEEEEECCCEEEEEEEEECCCCCCHHCCCCEEECCHHCCCCCCCC
LSPNDIEQKILGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYYSNSEKKHVNAPNYLID
CCHHHHHHHHHHHCCCCHHHCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCEEEE
ANSGEILKQWDEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLC
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCHHHCHHHCCCCCCCCCCCCEEEEECCCCEE
RVENESIKVMNLENHNIGYDFFPITTFAESVLNLSAFSYPCDETNLFLNYADGRTGPVNY
EECCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCE
AFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDMDNAFAVPTISLDGVVL
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCEEE
AHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSGGINESFSDMAAIALLD
EEEEEEEECCCCEEECCHHHHHHHHHCCCHHHHHCCEEEECCCCCCCCCHHHHHHHHHHH
YLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVF
HHHCCCCEEECCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCHHCCCEEEEEEECCHH
NKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEA
CCEEEEEEECCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FAEVGVVCPMHKS
HHHCCEECCCCCC
>Mature Secondary Structure
MKKRIVSLFVISTVISSVNAATQAIVWGESTKALPQFMQAHPFKKQGLLQTVKSQQSDYK
CCCHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEE
LELQNNSNHATRHARYQITYKGVPVWGYQVIFHSKDGAKETVTGMNITGIEQDINSTEGK
EEEECCCCCCEEEEEEEEEEECCCEEEEEEEEECCCCCCHHCCCCEEECCHHCCCCCCCC
LSPNDIEQKILGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYYSNSEKKHVNAPNYLID
CCHHHHHHHHHHHCCCCHHHCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCEEEE
ANSGEILKQWDEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLC
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCHHHCHHHCCCCCCCCCCCCEEEEECCCCEE
RVENESIKVMNLENHNIGYDFFPITTFAESVLNLSAFSYPCDETNLFLNYADGRTGPVNY
EECCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCE
AFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDMDNAFAVPTISLDGVVL
EECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCEEE
AHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSGGINESFSDMAAIALLD
EEEEEEEECCCCEEECCHHHHHHHHHCCCHHHHHCCEEEECCCCCCCCCHHHHHHHHHHH
YLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVF
HHHCCCCEEECCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCHHCCCEEEEEEECCHH
NKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEA
CCEEEEEEECCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FAEVGVVCPMHKS
HHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2045361; 10952301; 2123831 [H]