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The map label for this gene is wbmI [H]

Identifier: 148361288

GI number: 148361288

Start: 3512479

End: 3514302

Strand: Reverse

Name: wbmI [H]

Synonym: LPC_3262

Alternate gene names: 148361288

Gene position: 3514302-3512479 (Counterclockwise)

Preceding gene: 148361289

Following gene: 148361287

Centisome position: 98.26

GC content: 37.66

Gene sequence:

>1824_bases
ATGAATGCTTTATATGGAGTTCTGCGTTTTGACGGTCAACCATTTGAACGAGAGTTATTAGAGCGAGCGAAATTTAATAG
CCCGATATGGAAAGCGGATCATAGCGAACTTTATTATAATGATTTTTATGCTACGATGAGTTCACAGCGCTTTATTACCC
CCGAATGCGCCTTACAAACAGCACCATATTATGATAGGGAATCGGGTGTAATCGCCAATGGCGATTTATACCTAACTAAT
CATTCCAATTTGGCAAATCAGTTAGGAGTCAGTTCCAAAAATTCGGATTTGTACCTGGCAACGCAGGCTTATTTAAAGTG
GGGTGAAGAATGCGTGAATTATTTATCAGGGAATTTTTTAGTCGTATTTTATAATCCGAGTAAAAACAAGCTGTTTATTG
CCATTGATCACTTTAATTTTTGTTGCTGTTACTATCTGTTTGTTCCAGATAAATACTTTATATTTAGCAATACAATCAGT
ACCTTTAAATTTTTTAATGATTCCCTGACATTGAATAATGATGCTTTCTATAAGCTTGCTGCAAAATATGGACTAGGCCA
AGAAACTTGTCTGAAATCAGTTAAAAAACTGAGGGCTGCTAACTATATCATTGTTACCGACCAAAATTTACAAGAGAAAC
AATACTGGGATCTTCATAAGACGCAACACCCAAGCCATAAATCACGTGAAGATTACTATAGTTCATTCAGAAATGAGATT
CAGAAAAGTATCAGGCAAAGTATCCGGACTGACGCTGGAATTATGGCCCATTACAGCGGTGGTTTGGATTCTTCCTCCAT
TGCTTCGATTGCAGCATTGGAACTTGCAAAAGAAAATAAAACTCTTTTGGCATATACCGCGATTCCCAAATATTTGTCGG
GCGATAGTCATCGGGCAGGATGTAAATATCATGAATTAGATCGAGTGCAAAGCGCATGCCGTTACTATGACAATATTGAA
ACAATAGTTTATCAAAGCTCTGAAGACATGAGTATTTTTGATAGGCTTCAACCCTTTTATCCTTATTTAGATCAACCATT
CAAAGCGGTATTCAATATGGAATGGATATTGGCAGGATTGGAAATGGCAGCAGCCAAAAACTGCAGGATGCTTTTAAATG
GTGAACAGGGGAATGCCTCCATTTCATGGAGGGGAGTGACATTGGGCAACTATCTGAATCAGCGCGTAAAATTTACGCGG
CATTTTTTAAAATATCATATGAAATTAGGCAAGCAATTTATAAAATCTGGCTCTCTAAATCTGAAAAAGAATAGATTACA
TATTAATGGGATGAAAAAAATATTTTTAGACCCCCACTATTCTCTCTTGATGGGTAAGAACAGATTGGAAACAAGAAGTT
ACATGCGTTCCATTTATTTGTGGTATGGGGTAGAGGTGATTGACCCAACAAGTAACCTGGAGCTGGTCCAGTTTTGCTAT
GATCTTCCTGAATGGATTTATCATGGAGGTAACAAATATTTTGAAATCAGGCGATTGCCCAAATTAAAAAGACGCTTATT
GGTGAGAGATGCTTTGGAAGGGCTTGTGCCCCCTGAGATCCGCTATAATCCCTATCGCGGTGAACAGGCAGCAGATTGGT
ACTATCAATTCAATAAATATGGTCATGCTTGGGAAAGGCTTTTGGCAGATATTGCAGAGAAACACCCACTCATTTGGGAA
ATTTATCCTAAAAAATACCTTTTGGATTTATTTGCTAATCATCCCATTAATCCGCAATCAAGGCATTTTCAAGATGTTGA
AGCGCAGTTGATCCCTGCTTTAAGCACAGCATTTTTTATCAATGACCTAAATAACCTGTATTGA

Upstream 100 bases:

>100_bases
CAAATTTAGCGGTTTTTTTGCGGCGATTATATTTCTTGGTTAAGAAATTTAACAACGCCCTGGCGAGCAGTTATTTCTCT
TTTTTATCTTAGGATGTAGC

Downstream 100 bases:

>100_bases
CTAATATTTATTGGAGATTGTCTCGATGAGCAAGCATTTACAACAACACGAATATCAATTCGAATATAAAACTAATACAA
TACTAGAACGCCATGATTTG

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MNALYGVLRFDGQPFERELLERAKFNSPIWKADHSELYYNDFYATMSSQRFITPECALQTAPYYDRESGVIANGDLYLTN
HSNLANQLGVSSKNSDLYLATQAYLKWGEECVNYLSGNFLVVFYNPSKNKLFIAIDHFNFCCCYYLFVPDKYFIFSNTIS
TFKFFNDSLTLNNDAFYKLAAKYGLGQETCLKSVKKLRAANYIIVTDQNLQEKQYWDLHKTQHPSHKSREDYYSSFRNEI
QKSIRQSIRTDAGIMAHYSGGLDSSSIASIAALELAKENKTLLAYTAIPKYLSGDSHRAGCKYHELDRVQSACRYYDNIE
TIVYQSSEDMSIFDRLQPFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRMLLNGEQGNASISWRGVTLGNYLNQRVKFTR
HFLKYHMKLGKQFIKSGSLNLKKNRLHINGMKKIFLDPHYSLLMGKNRLETRSYMRSIYLWYGVEVIDPTSNLELVQFCY
DLPEWIYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRYNPYRGEQAADWYYQFNKYGHAWERLLADIAEKHPLIWE
IYPKKYLLDLFANHPINPQSRHFQDVEAQLIPALSTAFFINDLNNLY

Sequences:

>Translated_607_residues
MNALYGVLRFDGQPFERELLERAKFNSPIWKADHSELYYNDFYATMSSQRFITPECALQTAPYYDRESGVIANGDLYLTN
HSNLANQLGVSSKNSDLYLATQAYLKWGEECVNYLSGNFLVVFYNPSKNKLFIAIDHFNFCCCYYLFVPDKYFIFSNTIS
TFKFFNDSLTLNNDAFYKLAAKYGLGQETCLKSVKKLRAANYIIVTDQNLQEKQYWDLHKTQHPSHKSREDYYSSFRNEI
QKSIRQSIRTDAGIMAHYSGGLDSSSIASIAALELAKENKTLLAYTAIPKYLSGDSHRAGCKYHELDRVQSACRYYDNIE
TIVYQSSEDMSIFDRLQPFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRMLLNGEQGNASISWRGVTLGNYLNQRVKFTR
HFLKYHMKLGKQFIKSGSLNLKKNRLHINGMKKIFLDPHYSLLMGKNRLETRSYMRSIYLWYGVEVIDPTSNLELVQFCY
DLPEWIYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRYNPYRGEQAADWYYQFNKYGHAWERLLADIAEKHPLIWE
IYPKKYLLDLFANHPINPQSRHFQDVEAQLIPALSTAFFINDLNNLY
>Mature_607_residues
MNALYGVLRFDGQPFERELLERAKFNSPIWKADHSELYYNDFYATMSSQRFITPECALQTAPYYDRESGVIANGDLYLTN
HSNLANQLGVSSKNSDLYLATQAYLKWGEECVNYLSGNFLVVFYNPSKNKLFIAIDHFNFCCCYYLFVPDKYFIFSNTIS
TFKFFNDSLTLNNDAFYKLAAKYGLGQETCLKSVKKLRAANYIIVTDQNLQEKQYWDLHKTQHPSHKSREDYYSSFRNEI
QKSIRQSIRTDAGIMAHYSGGLDSSSIASIAALELAKENKTLLAYTAIPKYLSGDSHRAGCKYHELDRVQSACRYYDNIE
TIVYQSSEDMSIFDRLQPFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRMLLNGEQGNASISWRGVTLGNYLNQRVKFTR
HFLKYHMKLGKQFIKSGSLNLKKNRLHINGMKKIFLDPHYSLLMGKNRLETRSYMRSIYLWYGVEVIDPTSNLELVQFCY
DLPEWIYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRYNPYRGEQAADWYYQFNKYGHAWERLLADIAEKHPLIWE
IYPKKYLLDLFANHPINPQSRHFQDVEAQLIPALSTAFFINDLNNLY

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70926; Mature: 70926

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALYGVLRFDGQPFERELLERAKFNSPIWKADHSELYYNDFYATMSSQRFITPECALQT
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCCEECHHHHHCC
APYYDRESGVIANGDLYLTNHSNLANQLGVSSKNSDLYLATQAYLKWGEECVNYLSGNFL
CCCCCCCCCEEECCCEEEECCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEE
VVFYNPSKNKLFIAIDHFNFCCCYYLFVPDKYFIFSNTISTFKFFNDSLTLNNDAFYKLA
EEEECCCCCEEEEEEECCCEEEEEEEECCCEEEEEECCHHHHHEECCCEEECCCHHHHHH
AKYGLGQETCLKSVKKLRAANYIIVTDQNLQEKQYWDLHKTQHPSHKSREDYYSSFRNEI
HHHCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QKSIRQSIRTDAGIMAHYSGGLDSSSIASIAALELAKENKTLLAYTAIPKYLSGDSHRAG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCC
CKYHELDRVQSACRYYDNIETIVYQSSEDMSIFDRLQPFYPYLDQPFKAVFNMEWILAGL
CCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHH
EMAAAKNCRMLLNGEQGNASISWRGVTLGNYLNQRVKFTRHFLKYHMKLGKQFIKSGSLN
HHHHCCCCEEEEECCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LKKNRLHINGMKKIFLDPHYSLLMGKNRLETRSYMRSIYLWYGVEVIDPTSNLELVQFCY
EEECEEEECCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHH
DLPEWIYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRYNPYRGEQAADWYYQFNKY
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHC
GHAWERLLADIAEKHPLIWEIYPKKYLLDLFANHPINPQSRHFQDVEAQLIPALSTAFFI
CHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NDLNNLY
HHHHHCC
>Mature Secondary Structure
MNALYGVLRFDGQPFERELLERAKFNSPIWKADHSELYYNDFYATMSSQRFITPECALQT
CCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCCEECHHHHHCC
APYYDRESGVIANGDLYLTNHSNLANQLGVSSKNSDLYLATQAYLKWGEECVNYLSGNFL
CCCCCCCCCEEECCCEEEECCCHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEE
VVFYNPSKNKLFIAIDHFNFCCCYYLFVPDKYFIFSNTISTFKFFNDSLTLNNDAFYKLA
EEEECCCCCEEEEEEECCCEEEEEEEECCCEEEEEECCHHHHHEECCCEEECCCHHHHHH
AKYGLGQETCLKSVKKLRAANYIIVTDQNLQEKQYWDLHKTQHPSHKSREDYYSSFRNEI
HHHCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QKSIRQSIRTDAGIMAHYSGGLDSSSIASIAALELAKENKTLLAYTAIPKYLSGDSHRAG
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCCC
CKYHELDRVQSACRYYDNIETIVYQSSEDMSIFDRLQPFYPYLDQPFKAVFNMEWILAGL
CCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHH
EMAAAKNCRMLLNGEQGNASISWRGVTLGNYLNQRVKFTRHFLKYHMKLGKQFIKSGSLN
HHHHCCCCEEEEECCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LKKNRLHINGMKKIFLDPHYSLLMGKNRLETRSYMRSIYLWYGVEVIDPTSNLELVQFCY
EEECEEEECCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHH
DLPEWIYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRYNPYRGEQAADWYYQFNKY
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHC
GHAWERLLADIAEKHPLIWEIYPKKYLLDLFANHPINPQSRHFQDVEAQLIPALSTAFFI
CHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NDLNNLY
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]