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The map label for this gene is ksgA

Identifier: 148361259

GI number: 148361259

Start: 3487431

End: 3488201

Strand: Reverse

Name: ksgA

Synonym: LPC_3233

Alternate gene names: 148361259

Gene position: 3488201-3487431 (Counterclockwise)

Preceding gene: 148361263

Following gene: 148361258

Centisome position: 97.53

GC content: 38.26

Gene sequence:

>771_bases
GTGAGGCATAGCCCACGTAAACGTTTTGGTCAAAATTTCTTACAAGACAAATATATTATTAATGAGATTCTACGCGCTAT
CAATCCTTTGGCGGATGATAATATGCTGGAGATTGGCCCTGGTTTAGGCGCTTTAACTCAGCCATTATTGCAAAAACTGA
ATCGATTAACAGCCATTGAAATCGATACGGATTTACAAAACTATTTAACCCGCTTGCCCGTATCTCAAGGCAAACTGAAT
TTAATTCCTGCTGATGCGTTAACCGTTGACTTTTGTCAATTCGGCCCCCATTTAAGAGTAGTGGGTAATTTACCTTATAA
TATTTCTACTCCATTGCTGATCTATTTATTGAAATTTATAACTTGTATTGATGACATGCATTTTATGTTACAAAAAGAAG
TTGTAGAACGAATAGCAGCAACCCACGGGACTAAAGCCTATGGGCGACTAAGCGTGATGTTGCAGTATCATTGTGAGGTA
GAGTATTTATTTGATGTTCCTCCAGAAGCGTTTGAGCCCAAGCCCAAGGTAGACTCAGCAATAGTTCGATTAACACCTTA
TCGTGTTTCGCCATTTGAGTCTGTAAACACTGAGAAACTGGAAAATATTGTTGCGAAAGCATTTGCTATGAGACGCAAAA
CTCTTACTAATAATTTGAAAGGGATTATCTCATTATCTCAACTCAATGATTTAGGTATTGATGGAGGTAAGCGGCCAGAA
CAAATTTCGGTGGCGGAATATGTCCAACTAGCGAAATTTATTTCCAATTAG

Upstream 100 bases:

>100_bases
ACTAACCTACGGTAAAATTAACAGGCTTCCCCAATGGGGTATACCTTGTGATGTCAACAAGATTACTGAATTAATTTTAT
CCTAACATAGGTGATAGAAA

Downstream 100 bases:

>100_bases
TGTAAAATTATATGATAAGTTATTGTGGAGGTTGTCTTGTTTCATCGTCAGCGTAAAAGCTCTTTATCTGCTCAGTCCAA
GTCATTAAAAGATTTAATCC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIEIDTDLQNYLTRLPVSQGKLN
LIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEV
EYLFDVPPEAFEPKPKVDSAIVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
QISVAEYVQLAKFISN

Sequences:

>Translated_256_residues
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIEIDTDLQNYLTRLPVSQGKLN
LIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEV
EYLFDVPPEAFEPKPKVDSAIVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
QISVAEYVQLAKFISN
>Mature_256_residues
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIEIDTDLQNYLTRLPVSQGKLN
LIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEV
EYLFDVPPEAFEPKPKVDSAIVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
QISVAEYVQLAKFISN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=281, Percent_Identity=33.0960854092527, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI7657198, Length=252, Percent_Identity=32.1428571428571, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=49.8054474708171, Blast_Score=252, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI25146882, Length=210, Percent_Identity=34.7619047619048, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=244, Percent_Identity=28.6885245901639, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=217, Percent_Identity=27.1889400921659, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21358017, Length=218, Percent_Identity=34.8623853211009, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21357273, Length=287, Percent_Identity=29.9651567944251, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_LEGPA (Q5X0V1)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_125297.1
- ProteinModelPortal:   Q5X0V1
- SMR:   Q5X0V1
- STRING:   Q5X0V1
- GeneID:   3117441
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2995
- LegioList:   lpp2995
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   LPNE297246:LPP2995-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 28952; Mature: 28952

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 39-39 BINDING 60-60 BINDING 85-85 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHEEEE
IDTDLQNYLTRLPVSQGKLNLIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFI
ECHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
TCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEVEYLFDVPPEAFEPKPKVDSA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCEEEEECCCCHHCCCCCCCCCC
IVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCCCCCCC
QISVAEYVQLAKFISN
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHEEEE
IDTDLQNYLTRLPVSQGKLNLIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFI
ECHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
TCIDDMHFMLQKEVVERIAATHGTKAYGRLSVMLQYHCEVEYLFDVPPEAFEPKPKVDSA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCEEEEECCCCHHCCCCCCCCCC
IVRLTPYRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCCCCCCC
QISVAEYVQLAKFISN
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA