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The map label for this gene is dusB [H]

Identifier: 148361172

GI number: 148361172

Start: 3406435

End: 3407421

Strand: Direct

Name: dusB [H]

Synonym: LPC_3146

Alternate gene names: 148361172

Gene position: 3406435-3407421 (Clockwise)

Preceding gene: 148361160

Following gene: 148361175

Centisome position: 95.25

GC content: 40.53

Gene sequence:

>987_bases
ATGCACACTTTTATTAACAGTCCTCTGCAAATCGGCTCTTTAAAACTCCCTCATCGACTGATTCAAGGACCTTTGGCAGG
TTACAGTTGCGCCCCTTTCAGAGATCTATTCAATATTTATACACCGCCTGCTTATTGCGTGTCTGAAATGTGTTCTGCCA
TCGACATTCTTCATAAACACTCCTCTCAATCCCGGTATATTTATAGAGCCCCCACAGAAAAACTCCTGGCTTACCAAATC
TCCGGAAATGAGCCATATCTTCTTGCAGAAGCAGCAGCTAAACTACAATCACTGGGTGCAGATCTAATTGATATTAATTG
TGGCTGCCCTAAAAGCAAAATTCGTAAAAAAGGGGCTGGTAGCGCTTTATTGGAAGATCCACAAAAATTGATTGCCATTA
TCTCTAGGGTACGCGCAGCAATTAGCATTCCGCTGACTATTAAAATACGCATTCAAAATAATGAAAAGGATTTAACCTTA
GCCAAACAAATAGAGGACGCCGGAGCTCATGCCCTGATAGTACATGGCAGGCGATGGACAGAAGATTATGATATCCCATG
CAATTGGCAGCAAATTGCAAATATCAAGAATGCTGTCAACATACCAGTCATTGTAAATGGTGATATTAACAATATATTCA
GTTTAAGAAAGGCCCTGGAAATCAGCGCGTGCGATGGTTTTATGATTTCTCGAGCCGGTACAGGCAAACCCTGGCTATAT
CGAGACTTATTGAATCTGGAAACAAATGATGTCGCTTTTGATGAGAAATTAAAACTATTTATGCTCCACCTCGAAGGATT
GTCATACCTTGAAAACGAGCATAAAGCAGTACTGCAAAGTAAATCTCTGGTTCGCTATTACTTCAAAAAGCAACTGAACG
AACAACAACTGCAACAGTTCTATCAATTAGGTTCATTAATGGAAATCAAGGATTTTTTAGCTGAGGCAACTCACCGAGTT
TCCAACATATTAGTTGCCGCCTCATGA

Upstream 100 bases:

>100_bases
ACAAATACCTCCTGTCCATATGGAAGCCTCTCCCTTTTTTCTGGTTCTTCATGGTGAATCATGTTAAAATAAAAAGCGAT
TATATCCTTTAATTCCATTG

Downstream 100 bases:

>100_bases
TATAACGTCAGGATTTGTATATTATTTCTATAATTTGATAACCATTACATTCCGCTATTTAGTTCAGCTCACGTTGAATT
TCTCGGGCTTCCATTCTGGC

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKHSSQSRYIYRAPTEKLLAYQI
SGNEPYLLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWLY
RDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKAVLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHRV
SNILVAAS

Sequences:

>Translated_328_residues
MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKHSSQSRYIYRAPTEKLLAYQI
SGNEPYLLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWLY
RDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKAVLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHRV
SNILVAAS
>Mature_328_residues
MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKHSSQSRYIYRAPTEKLLAYQI
SGNEPYLLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWLY
RDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKAVLQSKSLVRYYFKKQLNEQQLQQFYQLGSLMEIKDFLAEATHRV
SNILVAAS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=278, Percent_Identity=26.2589928057554, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239788462, Length=283, Percent_Identity=25.7950530035336, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=29.0178571428571, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI8923374, Length=204, Percent_Identity=32.3529411764706, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI40807366, Length=181, Percent_Identity=31.4917127071823, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=305, Percent_Identity=31.4754098360656, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=31.6239316239316, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI145693211, Length=205, Percent_Identity=24.390243902439, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=295, Percent_Identity=27.7966101694915, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=220, Percent_Identity=30.9090909090909, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17510279, Length=181, Percent_Identity=35.3591160220994, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=159, Percent_Identity=27.6729559748428, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323437, Length=291, Percent_Identity=23.7113402061856, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=176, Percent_Identity=31.8181818181818, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=278, Percent_Identity=22.6618705035971, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580595, Length=177, Percent_Identity=31.638418079096, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920448, Length=177, Percent_Identity=31.638418079096, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585320, Length=241, Percent_Identity=29.4605809128631, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=26.4069264069264, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45549423, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36838; Mature: 36838

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKH
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHC
SSQSRYIYRAPTEKLLAYQISGNEPYLLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAG
CCCCCEEEECCCCCEEEEEECCCCCEEHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCC
SALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTLAKQIEDAGAHALIVHGRRWT
CHHHHCHHHHHHHHHHHHHHEECCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
EDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWLY
CCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
RDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKAVLQSKSLVRYYFKKQLNEQQLQQF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YQLGSLMEIKDFLAEATHRVSNILVAAS
HHHCCHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPAYCVSEMCSAIDILHKH
CCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHC
SSQSRYIYRAPTEKLLAYQISGNEPYLLAEAAAKLQSLGADLIDINCGCPKSKIRKKGAG
CCCCCEEEECCCCCEEEEEECCCCCEEHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCC
SALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTLAKQIEDAGAHALIVHGRRWT
CHHHHCHHHHHHHHHHHHHHEECCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCC
EDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKALEISACDGFMISRAGTGKPWLY
CCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
RDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKAVLQSKSLVRYYFKKQLNEQQLQQF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YQLGSLMEIKDFLAEATHRVSNILVAAS
HHHCCHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]