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The map label for this gene is folE2 [H]

Identifier: 148361079

GI number: 148361079

Start: 3293892

End: 3294431

Strand: Direct

Name: folE2 [H]

Synonym: LPC_3050

Alternate gene names: 148361079

Gene position: 3293892-3294431 (Clockwise)

Preceding gene: 148361078

Following gene: 148361105

Centisome position: 92.1

GC content: 37.78

Gene sequence:

>540_bases
ATGGAAAAATTATACTCAAATTTGCTTAAAGAATTAGGCGAAGATATAAATCGGGAAGGATTAAAGGATACTCCTGGACG
AGCAGCTAATGCCTTACGTTATTTAACTAAAGGTTACAATGAGAACCTTGATGAAATCATTAACGGTGCTCTGTTTGATT
CAGACATGAGTGAAATGGTTATCGTCAAGGACATAGAACTGTACTCATTATGCGAACATCACTTATTGCCTTTTTTGGGC
AAATGTCATGTTGGATATCTACCTGATGGTAAAGTAATAGGCTTATCTAAAATTGCTCGTATTGTTGATTTTTATGCGAG
GAGACTACAAATTCAAGAACGGCTGACTGGCGAAATTGCTCATTGTATTGAATCCATTACCGGAGCTCGCGGTGTCGCCG
TAGTGATTGAAGCAAAACATCTTTGCATGATGATGCGTGGAGTTGAAAAACAGAATTCTGTCATGACAACTTCAGTTATG
CTCGGTGAAATGCGTAACAATTCGAGTTGCCGTTTGGAATTCTTAAATTTAATCCGTTAA

Upstream 100 bases:

>100_bases
TTAATGTTAATTCTGGTGGCGGGCAGGAAGTTTACCACATTCATTTACATTTACTTGGCGGGCGTCAAATGACTTGGCCG
CCAGGTTAGGAATTCTTGCT

Downstream 100 bases:

>100_bases
TACTGATAAGAAATAGGTAGGGGCTTTATCCCCGCCTATAATAAAACAATTGCTCCATTTACTGCTGATAGCTAACCCGA
TATATTACATTAGCGTAATC

Product: hypothetical protein

Products: NA

Alternate protein names: GTP cyclohydrolase I 2; GTP-CH-I 2 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLDEIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLG
KCHVGYLPDGKVIGLSKIARIVDFYARRLQIQERLTGEIAHCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
LGEMRNNSSCRLEFLNLIR

Sequences:

>Translated_179_residues
MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLDEIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLG
KCHVGYLPDGKVIGLSKIARIVDFYARRLQIQERLTGEIAHCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
LGEMRNNSSCRLEFLNLIR
>Mature_179_residues
MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLDEIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLG
KCHVGYLPDGKVIGLSKIARIVDFYARRLQIQERLTGEIAHCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
LGEMRNNSSCRLEFLNLIR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=175, Percent_Identity=56.5714285714286, Blast_Score=221, Evalue=2e-58,
Organism=Homo sapiens, GI4503949, Length=175, Percent_Identity=56.5714285714286, Blast_Score=221, Evalue=2e-58,
Organism=Homo sapiens, GI66932972, Length=134, Percent_Identity=56.7164179104478, Blast_Score=174, Evalue=4e-44,
Organism=Homo sapiens, GI66932970, Length=134, Percent_Identity=56.7164179104478, Blast_Score=173, Evalue=6e-44,
Organism=Escherichia coli, GI1788476, Length=185, Percent_Identity=38.3783783783784, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=174, Percent_Identity=59.1954022988506, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321706, Length=173, Percent_Identity=57.8034682080925, Blast_Score=209, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24656791, Length=178, Percent_Identity=55.0561797752809, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=55.0561797752809, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24656796, Length=178, Percent_Identity=55.0561797752809, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20192; Mature: 20192

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLDEIINGALFDSDMSEMV
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEE
IVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIVDFYARRLQIQERLTGEIA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
HCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVMLGEMRNNSSCRLEFLNLIR
HHHHHHCCCCCEEEEEHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLDEIINGALFDSDMSEMV
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEE
IVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIVDFYARRLQIQERLTGEIA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
HCIESITGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVMLGEMRNNSSCRLEFLNLIR
HHHHHHCCCCCEEEEEHHHHHHHHHHCCCHHCHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]