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The map label for this gene is gadC [C]

Identifier: 148360980

GI number: 148360980

Start: 496973

End: 498376

Strand: Direct

Name: gadC [C]

Synonym: LPC_2940

Alternate gene names: 148360980

Gene position: 496973-498376 (Clockwise)

Preceding gene: 148360981

Following gene: 148360979

Centisome position: 13.9

GC content: 42.81

Gene sequence:

>1404_bases
ATGAGCAATAAAAAACATTCTCTCACTATATTTAGCTTGACTATGATTACCGTTGGCTCTGTGGACAGTATCCGTAATTT
GCCAGCTACTGCATTATTTGGGAGTCAATTGATTTTTTATTTTATCCTGGGTGCCTTATTTTTCCTTATCCCAACCGCAT
TGGTCTCGGCAGAGTTAGCTTCTGGGTGGGCCAGGCAGGGTGGCGTTTATATCTGGGTTAAGGAAGCTTTTGGGAAAAAA
TCAGGGTTTTTAGCCATTTGGTTGCAATGGATAGAAAATGTGATTTGGTATCCCACGATTCTATCGTTTGTTGCGGGAAC
AATAGGTTATCTTATTAATCCCTCTTTAACTACCAATCCTTATTTTCTGTGGGCAGTCATTGTCAGTTGCTTTTGGGGAG
CAACCATTGTGAATTTGCGCGGCATGAAATCATCGGCCATGTTCAGTAATATTTGTTCTTTGGCAGGATTATTATTACCC
ATGTCTTTGATCATTGGTTTGGGTGCTGTTTGGATAGCTCAAGGTAACCCCTTGCAAATCAGCTTTGACATGCCCAGTAT
TGTTCCCCATTTATCGGATAAGGGCATGTGGGTTTCATTAACCGCCATCATATTGTCATTTTGCGGGATAGAGATTGCTA
CAGTGCATGCTAATGATGTAAAGGATCCCCAGCATGCTTTCCCCAAAGCCCTGGTTTATTCTGTAGGGATCATACTGAGT
ACTTTGATTTTAGGTTCATTAGCGATTGCGGTAGTACTCCCACAAAAGGATATCAATCTGGTTGCCGGAATTATGCAAGC
CTTTTCTGCCTTTTTCCTTAAATATCACATGGATTGGATGATGCCTGTGGTCGCTGTGATGTTGGTTCTGGGAGGGCTTG
GTGGTGTGAGTAATTGGATCATTGCCCCTACCAAAGGTTTATTAGTTGCTGCAGAAGAAGGGAATTTACCGGAGGTATTT
CAACGGACTAATGGAAAAGGTGCTCCGGCCATGATGCTATACACCCAGGCAACCATAGTCACAGTATTATCGGCTTTATT
CTTATTTATGCCCAGTGTCAATGGATCCTACTGGCTCTTAACGGCATTGGCGGCTCAATTATACATGCTGATGTATTTGA
TCATGTTTGTCGCAGCAATTAAATTGCGCATAAGCCAACCTTACCATCCCAGACCTTTTAAAATTCCAGGTGGTATGGCA
GGAATGTTGTTTGTTGCAGGCATAGGCATCATTGGAGTGGTTACCACTTTAGCAGTTAGCTTTATTCCTCCAGAGGGGAT
TAATGTCGGCAGTGCAACACGTTATGAATTGACCTTGATTATTGGTTTGCTGCTCATGTGTGCCCCTCCTTTTATAACCA
GTTGGTTGCAAGCCAAAGAGGCGGATTTGGAACCTGCTGTATAA

Upstream 100 bases:

>100_bases
GGTATATTTGGTTATTTTTTATTCGATTATTAAGGTTTCCTTAAGCTTATTTCTTTATAATAAACTTTTGATTCCAAATA
GTCCTCACTGAAGGAATACA

Downstream 100 bases:

>100_bases
TTTAGCAAGACAGGTTAATTTTAATAACCCCAGTTGTTTATGTAATTTTCAAAAGATGGGCTATATTGACGAATTGTTGG
AAGAATTATCTGCACGATTG

Product: glutamate/gamma-aminobutyrate antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILGALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKK
SGFLAIWLQWIENVIWYPTILSFVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
MSLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHANDVKDPQHAFPKALVYSVGIILS
TLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYHMDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVF
QRTNGKGAPAMMLYTQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRPFKIPGGMA
GMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPPFITSWLQAKEADLEPAV

Sequences:

>Translated_467_residues
MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILGALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKK
SGFLAIWLQWIENVIWYPTILSFVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
MSLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHANDVKDPQHAFPKALVYSVGIILS
TLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYHMDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVF
QRTNGKGAPAMMLYTQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRPFKIPGGMA
GMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPPFITSWLQAKEADLEPAV
>Mature_466_residues
SNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILGALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKKS
GFLAIWLQWIENVIWYPTILSFVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLPM
SLIIGLGAVWIAQGNPLQISFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHANDVKDPQHAFPKALVYSVGIILST
LILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYHMDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVFQ
RTNGKGAPAMMLYTQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRPFKIPGGMAG
MLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPPFITSWLQAKEADLEPAV

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI115648063, Length=382, Percent_Identity=26.1780104712042, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI115648022, Length=382, Percent_Identity=26.1780104712042, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI186910308, Length=378, Percent_Identity=24.3386243386243, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI186910306, Length=378, Percent_Identity=24.3386243386243, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI186910304, Length=378, Percent_Identity=24.3386243386243, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI33286428, Length=378, Percent_Identity=24.8677248677249, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787768, Length=476, Percent_Identity=30.672268907563, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87081798, Length=397, Percent_Identity=24.1813602015113, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994921, Length=441, Percent_Identity=24.2630385487528, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357367, Length=375, Percent_Identity=23.7333333333333, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50494; Mature: 50363

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILGALFFLIPTALVSAELA
CCCCCCEEEEEEEEHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILSFVAGTIGYLINPSLTTNP
HHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLPMSLIIGLGAVWIAQGNPLQI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCEEE
SFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHANDVKDPQHAFPKALVYSVGIILS
EECCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
TLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYHMDWMMPVVAVMLVLGGLGGVSNWI
HHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLYTQATIVTVLSALFLFMPSVNGSYWLL
EECCCCEEEEECCCCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHH
TALAAQLYMLMYLIMFVAAIKLRISQPYHPRPFKIPGGMAGMLFVAGIGIIGVVTTLAVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIPPEGINVGSATRYELTLIIGLLLMCAPPFITSWLQAKEADLEPAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILGALFFLIPTALVSAELA
CCCCCEEEEEEEEHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILSFVAGTIGYLINPSLTTNP
HHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLPMSLIIGLGAVWIAQGNPLQI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCEEE
SFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHANDVKDPQHAFPKALVYSVGIILS
EECCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
TLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYHMDWMMPVVAVMLVLGGLGGVSNWI
HHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
IAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLYTQATIVTVLSALFLFMPSVNGSYWLL
EECCCCEEEEECCCCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHH
TALAAQLYMLMYLIMFVAAIKLRISQPYHPRPFKIPGGMAGMLFVAGIGIIGVVTTLAVS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FIPPEGINVGSATRYELTLIIGLLLMCAPPFITSWLQAKEADLEPAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]