Click here to switch to the map view.

The map label for this gene is eda [H]

Identifier: 148360963

GI number: 148360963

Start: 512033

End: 512695

Strand: Direct

Name: eda [H]

Synonym: LPC_2923

Alternate gene names: 148360963

Gene position: 512033-512695 (Clockwise)

Preceding gene: 148360964

Following gene: 148360962

Centisome position: 14.32

GC content: 43.74

Gene sequence:

>663_bases
ATGTCCTTTTCTGAATGGAAAACACAACCAGGCGCTGTATTTGCTGCATCTCCGGTTATTCCAGTCATTGTAATCAAAGA
GTTAGAGGATGTGTTACCTTTGGCCGAGGCTTTATTTGCCGGGGGGATTCATGTTCTTGAAGTGACATTAAGAACACCAG
TGGCAATAAAAGCACTGGAATTATTGATCAATACATTTCCAGATGAATTAATCGGTGCCGGAACTGTGATTACCCCGGGT
CAACTTCATGATGTGGCAGCAGCTGGCGCTCGATTTGCCATTAGCCCTGGCCAAACAAGGGAATTATTAATAGCAGGACA
GAAAAGTGAAATTCCTCTCATTCCAGGTGTAGCCAGTGTTTCTGAATTAATGGAGGGCTTGGGGATGGGATATAACCATT
TTAAATTTTTTCCCGCGGCAGCGGCTGGAGGTATCCCTATGCTGCAAGCGATATCGGGGGTATTCCCTCAAGTTAAATTT
TGTCCAACAGGCGGAATTAATTCCAAAAATTATGAGGAGTATCTTTGCTTGCCAAATGTAGTTTGCGTTGGAGGGTCCTG
GATTGTACCGGAAGAGGCTATCAAGAATCATAACTGGTCTTTAATTACAGAGTTATGTATGGCGGTGAGTTCTCAGAAGC
GAGAACTTTGCTCGCAGAATTAA

Upstream 100 bases:

>100_bases
GATTTTAATGCCTTAATTCCCACTTATGTCATTGCAGCCGCTCAACCAGGTATTTTAGGCGCGAGCGTCAGTTTAAAACA
AATATTGACAGGAGCTTCTC

Downstream 100 bases:

>100_bases
TCTATTCGAGTTGAAAAAATGTTTTCACGTGATGTAAGGGATAGTGCCAGCTAAAACAGTTCATTGTGTAGAGTAGAGAC
AGAGGAAAAGAATTTAAAAT

Product: bifunctional 2-keto-3-deoxygluconate 6- phosphate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSFSEWKTQPGAVFAASPVIPVIVIKELEDVLPLAEALFAGGIHVLEVTLRTPVAIKALELLINTFPDELIGAGTVITPG
QLHDVAAAGARFAISPGQTRELLIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKF
CPTGGINSKNYEEYLCLPNVVCVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN

Sequences:

>Translated_220_residues
MSFSEWKTQPGAVFAASPVIPVIVIKELEDVLPLAEALFAGGIHVLEVTLRTPVAIKALELLINTFPDELIGAGTVITPG
QLHDVAAAGARFAISPGQTRELLIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKF
CPTGGINSKNYEEYLCLPNVVCVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN
>Mature_219_residues
SFSEWKTQPGAVFAASPVIPVIVIKELEDVLPLAEALFAGGIHVLEVTLRTPVAIKALELLINTFPDELIGAGTVITPGQ
LHDVAAAGARFAISPGQTRELLIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKFC
PTGGINSKNYEEYLCLPNVVCVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=204, Percent_Identity=51.9607843137255, Blast_Score=204, Evalue=3e-54,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23383; Mature: 23252

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEWKTQPGAVFAASPVIPVIVIKELEDVLPLAEALFAGGIHVLEVTLRTPVAIKALE
CCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHH
LLINTFPDELIGAGTVITPGQLHDVAAAGARFAISPGQTRELLIAGQKSEIPLIPGVASV
HHHHHCCHHHHCCCCEECCCHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCCCCCHHHH
SELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKFCPTGGINSKNYEEYLCLPNV
HHHHHHHCCCCCHHEEECCHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCHHEECCCCE
VCVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN
EEECCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFSEWKTQPGAVFAASPVIPVIVIKELEDVLPLAEALFAGGIHVLEVTLRTPVAIKALE
CCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHH
LLINTFPDELIGAGTVITPGQLHDVAAAGARFAISPGQTRELLIAGQKSEIPLIPGVASV
HHHHHCCHHHHCCCCEECCCHHHHHHHCCCEEEECCCCCCEEEEECCCCCCCCCCCHHHH
SELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKFCPTGGINSKNYEEYLCLPNV
HHHHHHHCCCCCHHEEECCHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCHHEECCCCE
VCVGGSWIVPEEAIKNHNWSLITELCMAVSSQKRELCSQN
EEECCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]