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The map label for this gene is proP [H]

Identifier: 148360823

GI number: 148360823

Start: 654017

End: 655219

Strand: Direct

Name: proP [H]

Synonym: LPC_2782

Alternate gene names: 148360823

Gene position: 654017-655219 (Clockwise)

Preceding gene: 148360825

Following gene: 148360822

Centisome position: 18.29

GC content: 35.74

Gene sequence:

>1203_bases
ATGACAAATCTGGCTAGGATATCCGTTTTTATTAGTACTGCTATTGAAGTTTTTGATTTAAGCATCTTTGCTTTTCTTAT
TCCTGTGTTGTCTTCCGTATTTTTCTCATCCCATTCACAAACTTCAGCGATTAATTTTACCATTTTGGCTTATGTAGTAA
GCTATGTAGTGAAACCCTTTTCTGGAATGGTTTTTGGGTATCTGTCTGATAGTTATGGTCGAAAGCAAGTACTGTCAGTT
ACTACTTTGTTGATGACCGTTTCCACAGCGGTTATTGGTTTATTGCCTACCAGCCTACCTGGCATTGATTTATGGATAGC
ATTATTTAGTTGCCGGATTATTCAAGGGCTATCAATCTCAGGGGAGTTTTCTAATGGTTTAATATTTGCTGTTGAACAAG
GGAAAGATCGTCCGGCATTTTCTGGCAGTATGGCATTTATGGGCGGAATTCTTGGATTGTTGCTGGCAAATTTATCGGTT
TTTATATTACTAAACCATTTACCTTATGAGCAGATGATTCAATATGGCTGGCGTATACCATTTTTAATTAGTGCTATTAT
TTGGCTGGCACTGTACCAGATTAGAAGGTTTATTAATGAGCCCTTTGCGCAGAAGATGAATGATACTAAACATTTTTCAT
CTTTGGTAAAAAAATATAGAAAGGAGCTGGTTATTTGTTTTGTAGCTGCTAGTTTATCTGCTTCTGCTTTCTATATGACC
TTTGTTTATATGCCAACTCTTTTATCTTCAGTGATTCAAAATCAGACTCACCATAAATCTGTATGGGTGACGTTGATTGC
TTTGTCAGTCTATTTCATCTGTTTGCCCCTGTTTGGTTCTTTGGCCGATCGGGTAGGTATTACTAAGCAAATCACTGCTG
CTTCAGCATTGTACCTTTTATTTTCCTATGTCTGTTTTGACTATATTAGCCATTTTAACTATGTCGTTATATTTTGTAGT
TTGATATTACTGGCTTTGATTCAATCATTATATAATTCGGCTTTGCCTGCATTTATGGTTTCTTTGTTCCCCGCCATTCA
TCGAGGTAAGGCTTTAGCAATATCTTATAATACCAGCTTATCAATTTTTGGTGGTTTAATGCCCTATGTTATATTAAGCC
ATAAAAGTTTTATGAATCCGGGAATTGTTATTAGTATTTGTGCGATATTAACATTGGTTGTATTACGTTTTGTGAGGGAG
TAA

Upstream 100 bases:

>100_bases
TGATCTTGCCCTGACATCATGTATCATAACAAATCCTTAGAGGTACAGCTTAAATCTATTTAATGCTAAAATAATGTACG
ACACAATGAAGTGAATGTAA

Downstream 100 bases:

>100_bases
ACATGGTTGTATATGAAGTGAATCTATCTATTGATAATGAAATATATAGAGATTATAAGGATTGGTTAGACGAGCATATT
CTTGAAATGCTTAAATTTCC

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTNLARISVFISTAIEVFDLSIFAFLIPVLSSVFFSSHSQTSAINFTILAYVVSYVVKPFSGMVFGYLSDSYGRKQVLSV
TTLLMTVSTAVIGLLPTSLPGIDLWIALFSCRIIQGLSISGEFSNGLIFAVEQGKDRPAFSGSMAFMGGILGLLLANLSV
FILLNHLPYEQMIQYGWRIPFLISAIIWLALYQIRRFINEPFAQKMNDTKHFSSLVKKYRKELVICFVAASLSASAFYMT
FVYMPTLLSSVIQNQTHHKSVWVTLIALSVYFICLPLFGSLADRVGITKQITAASALYLLFSYVCFDYISHFNYVVIFCS
LILLALIQSLYNSALPAFMVSLFPAIHRGKALAISYNTSLSIFGGLMPYVILSHKSFMNPGIVISICAILTLVVLRFVRE

Sequences:

>Translated_400_residues
MTNLARISVFISTAIEVFDLSIFAFLIPVLSSVFFSSHSQTSAINFTILAYVVSYVVKPFSGMVFGYLSDSYGRKQVLSV
TTLLMTVSTAVIGLLPTSLPGIDLWIALFSCRIIQGLSISGEFSNGLIFAVEQGKDRPAFSGSMAFMGGILGLLLANLSV
FILLNHLPYEQMIQYGWRIPFLISAIIWLALYQIRRFINEPFAQKMNDTKHFSSLVKKYRKELVICFVAASLSASAFYMT
FVYMPTLLSSVIQNQTHHKSVWVTLIALSVYFICLPLFGSLADRVGITKQITAASALYLLFSYVCFDYISHFNYVVIFCS
LILLALIQSLYNSALPAFMVSLFPAIHRGKALAISYNTSLSIFGGLMPYVILSHKSFMNPGIVISICAILTLVVLRFVRE
>Mature_399_residues
TNLARISVFISTAIEVFDLSIFAFLIPVLSSVFFSSHSQTSAINFTILAYVVSYVVKPFSGMVFGYLSDSYGRKQVLSVT
TLLMTVSTAVIGLLPTSLPGIDLWIALFSCRIIQGLSISGEFSNGLIFAVEQGKDRPAFSGSMAFMGGILGLLLANLSVF
ILLNHLPYEQMIQYGWRIPFLISAIIWLALYQIRRFINEPFAQKMNDTKHFSSLVKKYRKELVICFVAASLSASAFYMTF
VYMPTLLSSVIQNQTHHKSVWVTLIALSVYFICLPLFGSLADRVGITKQITAASALYLLFSYVCFDYISHFNYVVIFCSL
ILLALIQSLYNSALPAFMVSLFPAIHRGKALAISYNTSLSIFGGLMPYVILSHKSFMNPGIVISICAILTLVVLRFVRE

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=26.6990291262136, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788942, Length=376, Percent_Identity=27.1276595744681, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788292, Length=380, Percent_Identity=24.4736842105263, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789941, Length=414, Percent_Identity=27.536231884058, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082404, Length=381, Percent_Identity=23.6220472440945, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44443; Mature: 44312

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLARISVFISTAIEVFDLSIFAFLIPVLSSVFFSSHSQTSAINFTILAYVVSYVVKPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
SGMVFGYLSDSYGRKQVLSVTTLLMTVSTAVIGLLPTSLPGIDLWIALFSCRIIQGLSIS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
GEFSNGLIFAVEQGKDRPAFSGSMAFMGGILGLLLANLSVFILLNHLPYEQMIQYGWRIP
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHH
FLISAIIWLALYQIRRFINEPFAQKMNDTKHFSSLVKKYRKELVICFVAASLSASAFYMT
HHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVYMPTLLSSVIQNQTHHKSVWVTLIALSVYFICLPLFGSLADRVGITKQITAASALYLL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FSYVCFDYISHFNYVVIFCSLILLALIQSLYNSALPAFMVSLFPAIHRGKALAISYNTSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
SIFGGLMPYVILSHKSFMNPGIVISICAILTLVVLRFVRE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNLARISVFISTAIEVFDLSIFAFLIPVLSSVFFSSHSQTSAINFTILAYVVSYVVKPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]