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The map label for this gene is xthA [H]

Identifier: 148360698

GI number: 148360698

Start: 802257

End: 803027

Strand: Direct

Name: xthA [H]

Synonym: LPC_2645

Alternate gene names: 148360698

Gene position: 802257-803027 (Clockwise)

Preceding gene: 148360699

Following gene: 148360697

Centisome position: 22.43

GC content: 42.15

Gene sequence:

>771_bases
GTGTTTAAACTCGCTAGCTGGAATGTTAATTCATTAAAAGTCCGTCTGGAACAGGTTTTGCAATGGCTTGAGTCTTCACA
AGTTGATGTGCTTGCCATGCAGGAGACTAAGCTGACTGATGACAACTTTCCTGTTGCTTCATTTACAGACAAAGGGTACC
ACGTCATTTTTTCAGGTCAGAAAACTTATAACGGGGTGGCCATTATTAGTCGATATCCTATGACAGATATTCAAACCGAT
ATTCCAGAACTGGAAGATCCCCAGCGGCGTATCCTGGCTGCTACGGTAAAAGGAATCAGATTGATTAATCTTTATGTGCC
TAATGGTGGAGAACTGACTTCAGACAAGTACCAATATAAACTGGATTGGTTAAAGAAAGTAACTCGCTTCATTCAACATC
AGTTAAGCATTTATTCTAAAGTAGCAGTTGTGGGTGATTTTAATATTGCGCCCGAAGATCGGGATGTCCATGATCCAGTT
GAGTGGGAGGGTTGTGTTTTGGTCAGTCCTGCTGAAAGGCAAGCATTTATGGAGATACTTGAATTGGGTTTACATGACAG
TTTCCGTAACTTTGCTCAGGAAGAAAAAGCGTTTAGCTGGTGGGACTATAGAGCCGCAGCATTCCGACGTAATCGTGGTT
TGCGCATTGATCATGTGCTATTGAGCCATGAATTAAACGCTTTATGCCGACAATCCAAAATCGATAAAGAACCAAGAAAA
GCAGAGAGGCCTTCCGATCATGCGCCGGTTTGGGTTGAGTTAGAATTATAA

Upstream 100 bases:

>100_bases
ACGCCTGGTCTGACTGAATATGTCGCGGACAGCCGAATTTTTCGCGAAGCACGTTTTTCGGATCATGCCCCTTTAATGAT
TGAATACAAGGGAGACTGGT

Downstream 100 bases:

>100_bases
ATGTCCGAGGTGTTTGAGAAAAAATTGCTTTATGAGATCATTTCTGCTATTGTTTTGACTCATTTGTGTAGAGTATGGTG
CTTGGCAATAGCGTCCAGGT

Product: hypothetical protein

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDDNFPVASFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTD
IPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYKLDWLKKVTRFIQHQLSIYSKVAVVGDFNIAPEDRDVHDPV
EWEGCVLVSPAERQAFMEILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
AERPSDHAPVWVELEL

Sequences:

>Translated_256_residues
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDDNFPVASFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTD
IPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYKLDWLKKVTRFIQHQLSIYSKVAVVGDFNIAPEDRDVHDPV
EWEGCVLVSPAERQAFMEILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
AERPSDHAPVWVELEL
>Mature_256_residues
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDDNFPVASFTDKGYHVIFSGQKTYNGVAIISRYPMTDIQTD
IPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYKLDWLKKVTRFIQHQLSIYSKVAVVGDFNIAPEDRDVHDPV
EWEGCVLVSPAERQAFMEILELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
AERPSDHAPVWVELEL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=30.6818181818182, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=30.6818181818182, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=30.6818181818182, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=35.6060606060606, Blast_Score=157, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=25.4752851711027, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=28.9473684210526, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=28.9473684210526, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29676; Mature: 29676

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDDNFPVASFTDKGYHVIFSGQ
CCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECC
KTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYK
CCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHH
LDWLKKVTRFIQHQLSIYSKVAVVGDFNIAPEDRDVHDPVEWEGCVLVSPAERQAFMEIL
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
ELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHH
AERPSDHAPVWVELEL
HCCCCCCCCEEEEEEC
>Mature Secondary Structure
MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDDNFPVASFTDKGYHVIFSGQ
CCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECC
KTYNGVAIISRYPMTDIQTDIPELEDPQRRILAATVKGIRLINLYVPNGGELTSDKYQYK
CCCCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHH
LDWLKKVTRFIQHQLSIYSKVAVVGDFNIAPEDRDVHDPVEWEGCVLVSPAERQAFMEIL
HHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
ELGLHDSFRNFAQEEKAFSWWDYRAAAFRRNRGLRIDHVLLSHELNALCRQSKIDKEPRK
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCHH
AERPSDHAPVWVELEL
HCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]