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The map label for this gene is pleD [H]

Identifier: 148360603

GI number: 148360603

Start: 922131

End: 923681

Strand: Direct

Name: pleD [H]

Synonym: LPC_2549

Alternate gene names: 148360603

Gene position: 922131-923681 (Clockwise)

Preceding gene: 148360604

Following gene: 148360602

Centisome position: 25.78

GC content: 34.88

Gene sequence:

>1551_bases
ATGATTGATCAAGAATTAAAGAAAAAAATTGTTAACACAATTAGCCAATATGAGCACAAATGTGACAAGATGCAAAATCA
AATTAATACGCTCAGAAATGTGATTAATCAATTGATTATTAATCCCATTGGAATTAATTATGAGTTGGATGATCGCCTTT
CGCAACTAAGAAATTATTTGGATACTGAATTTGAACCTGCTGTCATAGAAAAAAAAGTGAAACGTTTGGCAGAAATACTT
TCCAAAATGCAAAGGAAAAAAGCAGAAAATTCTCGTTTAGTCAATGGACTAATCAAACAGGGCGTGGAATCCATTGGTCG
TATTGCCAATAAATCTCAGGATAAGAGAGCCGTTACAAAATTACAAAAAATGCTGGATACCGAGATGGAAAGCCATGCGA
TTTTGATTCGTTTTAATCAAGTCTTAACTCAATGTATTTCTTCTGTGATTAAGGAGCTGGAGGAGTTACCACAAATTACA
AAAGTCGATAACCCCTCCAAAAGTTATCAATCTGAGATTAGTTTAAAAGTAAATGACAGCTTGCAACAATTACTTAACCA
CTTATCTATCCCTCAGGACTTGGATTCCAAGAGGGGAATCATAAAAACAAATTTGGAACATGCACTCACCGATGAAGAGC
TTAGCACTATTATTGACAATTTGACGGAATTAGTCGTTGAGTCATTTAACGTTGAGCAGAATCGATTCAAAGGGTTTCTT
CAACAGTTAACTGATCAGCTCAATGATTTTGAAATCTATTTAAGAGTATCAGGAGATGCTCGCAAAAAGGCAAGTGAAGA
CAGTCGTTTATTAGAGTGTGGAATTCAGGATAATCTTGATCAAATCAAGAATCATTTGGATACCTCTAAAACGATTGAGG
AATTATCTTCAAAGGTGAGCCAAAATATAAAAATGATTGGTGACAGGTTAAAAGAATATCGACACAATGAAAAAATCCGA
GAGGAAGAATATGCAAAGCAAGTATCCGAGTTGCAAGCTAGATTACAGGAATCCGAGCAAAATGCGGAAGAAATAAAAAA
TTTGCTTGCTTTTCAGAAATACAGAATTAACCACGATAGTTTAACAGGTTTACCTAATAGAGAATCTTATGAGGAGCATA
TTCTGGACTCATTTCAGCGCTGGAAACGAAGTTCAAGCGCATTGTCTCTTGCTGTGTGTGACATCGATCATTTTAAACGT
ATTAATGATAATTTTGGCCATCTTGCAGGAGATAAGGTTCTTAAAAAGGTAGCTATGATTTTTAAATCATCGATCAGGAC
GGTTGATTTTATCGCTCGAATAGGTGGTGAGGAATTTGTGTTTATCTTTGAACAAACTAGCAGCAAGGAAGCTTTTGGAA
TTTTGGAGAAATTACGTAAATTAGTTGAAGATTGTTTGTTTTATTATCGAGAGAAAAAAGTCGATGTTACGGTTTCTTTT
GGGCTAACTACGGTAACCGAAAAAGATGATATTGAATCTTTATTTATGCGGGCAGACAATGCCATGTATAAAGCTAAAAA
TGCAGGAAGGAACAGGGTAGAAATATTATAG

Upstream 100 bases:

>100_bases
CAAACCAAAAGCCCTCCTTTTTGTGATGGATCGCATGCAAAGATTTTGCTTGAAGTGGTTAAAAAAAGGCAAAATAAATG
AACGGGAAAGGAAGTCTTAA

Downstream 100 bases:

>100_bases
TGGAATATATTTCCAACAATTTTTAATTATACTATTTTCGGAGTAAATCCATGGTAACAGATGATTTTCCTCTGATAACG
ATTTTTAAATGAACTTGATC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQIT
KVDNPSKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSALSLAVCDIDHFKR
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYREKKVDVTVSF
GLTTVTEKDDIESLFMRADNAMYKAKNAGRNRVEIL

Sequences:

>Translated_516_residues
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQIT
KVDNPSKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSALSLAVCDIDHFKR
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYREKKVDVTVSF
GLTTVTEKDDIESLFMRADNAMYKAKNAGRNRVEIL
>Mature_516_residues
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIGINYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEIL
SKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQIT
KVDNPSKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIR
EEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSALSLAVCDIDHFKR
INDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYREKKVDVTVSF
GLTTVTEKDDIESLFMRADNAMYKAKNAGRNRVEIL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=36.0248447204969, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=175, Percent_Identity=36.5714285714286, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=37.8048780487805, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=32.2033898305085, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=34.9693251533742, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=130, Percent_Identity=37.6923076923077, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=31.6455696202532, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787541, Length=183, Percent_Identity=31.6939890710383, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=37.3015873015873, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=35.3658536585366, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=203, Percent_Identity=32.0197044334975, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=247, Percent_Identity=26.7206477732794, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081974, Length=148, Percent_Identity=27.7027027027027, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59726; Mature: 59726

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIGINYELDDRLSQLRNYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQITKVDNPSKSYQSEISLKVNDS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHEEEECHH
LQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVS
HHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHH
QNIKMIGDRLKEYRHNEKIREEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
LTGLPNRESYEEHILDSFQRWKRSSSALSLAVCDIDHFKRINDNFGHLAGDKVLKKVAMI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHH
FKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYREKKVDVTVSF
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GLTTVTEKDDIESLFMRADNAMYKAKNAGRNRVEIL
CCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIGINYELDDRLSQLRNYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGVESIGRIANKSQDKRAVTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQITKVDNPSKSYQSEISLKVNDS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHEEEECHH
LQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTIIDNLTELVVESFNVEQNRFKGFL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQDNLDQIKNHLDTSKTIEELSSKVS
HHHHHCCCCEEEEEEECCCHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHH
QNIKMIGDRLKEYRHNEKIREEEYAKQVSELQARLQESEQNAEEIKNLLAFQKYRINHDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
LTGLPNRESYEEHILDSFQRWKRSSSALSLAVCDIDHFKRINDNFGHLAGDKVLKKVAMI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHH
FKSSIRTVDFIARIGGEEFVFIFEQTSSKEAFGILEKLRKLVEDCLFYYREKKVDVTVSF
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GLTTVTEKDDIESLFMRADNAMYKAKNAGRNRVEIL
CCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA