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The map label for this gene is hypB [H]

Identifier: 148360072

GI number: 148360072

Start: 2966927

End: 2967685

Strand: Reverse

Name: hypB [H]

Synonym: LPC_2003

Alternate gene names: 148360072

Gene position: 2967685-2966927 (Counterclockwise)

Preceding gene: 148360071

Following gene: 148360073

Centisome position: 82.98

GC content: 39.53

Gene sequence:

>759_bases
ATGTGCGGGATATGCGGATGTTCAGTAGAAAAAGGTCAAACCATGGATAATGAGCACCATGCTACTCACCATGAGCATTC
TGTGCCTCATGAACATTCAATTGATATCGAACAGAATATTTTAGCGCAAAACAATGAATTTGCTGCTCATAACCGGGATT
ATTTTCTCAGGCATAAAATTTTGGTTTTGAATTTGATGTCAAGTCCTGGATCCGGGAAGACAACATTGCTTTCCAAAACC
ATTTCAGATCTTGGCAAGGAAATGGATATTGCAGTCATAGTAGGTGATCAACAAACAAATTGTGATGCCGAAATGCTAAA
ATCCAGTGGAGGGAATGCCTGGCAAATTAATACTGGAAAAGTCTGCCATTTGGATGCCCATATGGTGAGTCATGCCATAC
AAGATATTTCTCTAAAAAAGCAATCGCTTTTATTTATCGAAAATGTGGGTAATCTCGTTTGTCCGGCTTCATTTCATCTG
GGCGAGCAATTCAGAATAGTGACTCTTTCTGTGACAGAAGGAGAGAATAAACCGCTCAAATACCCTGATATGTTTCATTC
AGCAGATTTGCTCCTGATAACCAAAGCAGATTTATTACCCCATGTGAATTTTGATTTGGATTTATGTAAGGAATATGTAC
GCCGCACGAATCCATCCATAGACATTATTACCCTGTCAGCGGTTAATGGTGTTGGATTGAAGGATTGGTACGATTGGTTG
TTGCATTCTTTTAGGTTGAATGTCCAGGCTTCTTCATAG

Upstream 100 bases:

>100_bases
CAATGAAACAGTATTATGATGAATGCCTGGTTTGCGGCAATCATTCCTTAACGCTGACAAAAGGTGAAGAATTAAAAGTG
AAATCAATGGTGGTGGAATA

Downstream 100 bases:

>100_bases
AAAAAGTGGTAGCCCATGGAATTGGAACGGGTACAAATCAGCATTCAAGGACTAGTGCAAGGGGTTGGTTTTAGACCGTG
CGTTTATAAGCTTGCCAAAC

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQNNEFAAHNRDYFLRHKILVLNLMSSPGSGKTTLLSKT
ISDLGKEMDIAVIVGDQQTNCDAEMLKSSGGNAWQINTGKVCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHL
GEQFRIVTLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNPSIDIITLSAVNGVGLKDWYDWL
LHSFRLNVQASS

Sequences:

>Translated_252_residues
MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQNNEFAAHNRDYFLRHKILVLNLMSSPGSGKTTLLSKT
ISDLGKEMDIAVIVGDQQTNCDAEMLKSSGGNAWQINTGKVCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHL
GEQFRIVTLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNPSIDIITLSAVNGVGLKDWYDWL
LHSFRLNVQASS
>Mature_252_residues
MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQNNEFAAHNRDYFLRHKILVLNLMSSPGSGKTTLLSKT
ISDLGKEMDIAVIVGDQQTNCDAEMLKSSGGNAWQINTGKVCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHL
GEQFRIVTLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNPSIDIITLSAVNGVGLKDWYDWL
LHSFRLNVQASS

Specific function: Could be involved in nickel binding and accumulation [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=243, Percent_Identity=48.1481481481481, Blast_Score=234, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 28079; Mature: 28079

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQNNEFAAHNRDYFLRHKI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHEEE
LVLNLMSSPGSGKTTLLSKTISDLGKEMDIAVIVGDQQTNCDAEMLKSSGGNAWQINTGK
EEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEECCCC
VCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHLGEQFRIVTLSVTEGENKPLK
EEEEHHHHHHHHHHHHHCCHHHEEEEECCCCEECCCCCCCCCEEEEEEEEEECCCCCCCC
YPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNPSIDIITLSAVNGVGLKDWYDWL
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHH
LHSFRLNVQASS
HHHHEEEEEECC
>Mature Secondary Structure
MCGICGCSVEKGQTMDNEHHATHHEHSVPHEHSIDIEQNILAQNNEFAAHNRDYFLRHKI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHEEE
LVLNLMSSPGSGKTTLLSKTISDLGKEMDIAVIVGDQQTNCDAEMLKSSGGNAWQINTGK
EEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCCEEEECCCC
VCHLDAHMVSHAIQDISLKKQSLLFIENVGNLVCPASFHLGEQFRIVTLSVTEGENKPLK
EEEEHHHHHHHHHHHHHCCHHHEEEEECCCCEECCCCCCCCCEEEEEEEEEECCCCCCCC
YPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNPSIDIITLSAVNGVGLKDWYDWL
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHH
LHSFRLNVQASS
HHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486288 [H]