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The map label for this gene is mscL [H]

Identifier: 148359998

GI number: 148359998

Start: 3045533

End: 3045916

Strand: Reverse

Name: mscL [H]

Synonym: LPC_1928

Alternate gene names: 148359998

Gene position: 3045916-3045533 (Counterclockwise)

Preceding gene: 148359997

Following gene: 148359999

Centisome position: 85.17

GC content: 36.46

Gene sequence:

>384_bases
ATGAGTTTACTAAAGGAATTTAAAGAATTTGCCATGCGAGGCAACGTGATGGATTTGGCTGTTGCTGTTGTAATGGGTGT
TGCTTTTAATAAAATTGTCACTGCTTTGGTCGATGGAATTATCATGCCCTGCGTTGGTTTGTTATTGGGAGGCGTCAATA
TTGCAGGATTGAGTTTTACTGTAGGAGACGCTCAAATCAAGTGGGGAAATTTTTTACAAAACGTCATTGATTTTATTATC
GTTGCTTTTGCTATTTTTGTACTCATCAAATTAATCAATCTGCTGCAAAGAAAAAAAGCAAATGAACCAGAACCAGTCAC
TTCGGAAATCCAATTACTTACTGAAATACGTGATCTGCTTGCAAGAAATAGCAGTAAAATCTGA

Upstream 100 bases:

>100_bases
TACAAGCTAACAGGCGTCAGAACAATCTGTAATTGAAAGATTGGAAAAATTTTGCGAATATACATTTCATCGCATAACTT
AATCATGATAGGGATGTCTG

Downstream 100 bases:

>100_bases
AATTGGCTGGCAAAAATAAACCTCTATTTATTCGCAGTCAGATACCCCCATTCAAAATGGCTTGTTAAACAGCTTATGTT
TTATTTCGGTGAGATGTATA

Product: large conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPCVGLLLGGVNIAGLSFTVGDAQIKWGNFLQNVIDFII
VAFAIFVLIKLINLLQRKKANEPEPVTSEIQLLTEIRDLLARNSSKI

Sequences:

>Translated_127_residues
MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPCVGLLLGGVNIAGLSFTVGDAQIKWGNFLQNVIDFII
VAFAIFVLIKLINLLQRKKANEPEPVTSEIQLLTEIRDLLARNSSKI
>Mature_126_residues
SLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPCVGLLLGGVNIAGLSFTVGDAQIKWGNFLQNVIDFIIV
AFAIFVLIKLINLLQRKKANEPEPVTSEIQLLTEIRDLLARNSSKI

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=55.1470588235294, Blast_Score=137, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 13889; Mature: 13758

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPCVGLLLGGVNIAGLSFT
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEE
VGDAQIKWGNFLQNVIDFIIVAFAIFVLIKLINLLQRKKANEPEPVTSEIQLLTEIRDLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ARNSSKI
HCCCCCC
>Mature Secondary Structure 
SLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPCVGLLLGGVNIAGLSFT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEE
VGDAQIKWGNFLQNVIDFIIVAFAIFVLIKLINLLQRKKANEPEPVTSEIQLLTEIRDLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ARNSSKI
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA