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The map label for this gene is dnaG [H]

Identifier: 148359901

GI number: 148359901

Start: 2777782

End: 2779515

Strand: Direct

Name: dnaG [H]

Synonym: LPC_1829

Alternate gene names: 148359901

Gene position: 2777782-2779515 (Clockwise)

Preceding gene: 148359900

Following gene: 148359902

Centisome position: 77.67

GC content: 39.5

Gene sequence:

>1734_bases
ATGTCTGGCTTAATCCCACAGCCATTCATTGATGATCTATTAAACCGCACTGATTTGGTGGAATTAATAGACGGATATGT
TCCTTTGAAAAAAAGAGGCAACAGTCATATTGCGTGTTGCCCTTTCCATAATGAGAAAACACCATCATTCAATGTCGTTG
CAAAAAAACAATTTTATCATTGTTTTGGTTGTGGCGCTTCAGGTAACGCCATCAGTTTTGTAATGAATTATCTGAATCAA
GGTTTTACGGATGCCGTTGAAACCCTCGCGACACGCTTGGGTTTAAGTGTCCCAAGGGATGGTACAGGAGAGAAACATAA
ATCATCTCTCAATCTCTATAATCTCATGTCAGAAGTCAGCCAATATTACCAAAAAAAATTAAAATATAATGGACAAACAG
CAATTGAATATCTTCGCAACCGAGGCCTTAGTGGGGAAATTGCCAAACGATATCATTTAGGCTATGCACCTGAAGGTTGG
CATAACCTGGAAAAAGCTTTCCCGAACAATCAACGTGAACTATTGAGTACCGGTATGCTCATTAAAAGCGATGAAGGTAA
AATTTATGACAGATATCGTAATCGAATTATGTTCCCCATCCATGACAGAAACGGTCGTGTGATTGGCTTTGGCGGACGTG
TACTCGATAAAGATCAAAAGCCCAAATACCTTAACTCGCCTGAAACAGTACTTTTTCAAAAAAGCCGTGAGTTATATGGC
TTACATCAAATCCTGTCTCAGCAGAAAAATCCCGACAGTATTATTGTTGTTGAAGGTTACATGGATGTCATTGCCTTGGC
ACAACATGGCATTTTTAATGTGGTGGCAACGTTGGGAACTGCCACCAGCACATTCCATATACAACTATTGGCAAAGCATA
CCAAACATCTCATTTTTTGCTTTGATGGCGATGCGGCAGGTAAACAAGCAGCCTGGCGAGCATTGGAGAGCAGTTTACCG
CATTTGAATGCCGGATTAGATGCTGGATTTATTTTCTTGCCGAATGAACATGACCCGGATAGCCTGGTAAGAAATGAAGG
AAAAAACGGTTTCCTTGAGCTCTTGCAACAAGCAACACCACTCCACCGCTTTTTCTTTGATACTTTAAGTAAAGACATTA
ATTTGTCAAACCCTGCAGGAAAAACACAGCTTATCAACGCAGTGAAACCCTATTTGCAAAAAATGGTTGAGGGCTCATAC
AAACAGTTGCTTATTGATGACCTGGCACGCTTAACCCATATTGAAAGCCATCGGTTAACTAATTTGATTACTGATAAATC
GGAAAGCAAGCCGGAAGTACAAGCTGCTATTTCTCGTTCACCTCTGCGTATTGCTATCGCGCTCCTCTTACAAAACCCCG
AAATTTATTCTATAGCAATACAACAAATCAATCCTGCTCTGCTCCATGAACAGGAGCATCATATTTTATTGAAATTATTG
CAACAATTAAAAGACAAGCCCAATGCCAATACTGCCACCCTCATTGAATTCTGGCGCAATAGCAGTTATTTTGAATTGAT
AGTTAAATTGGCGGCCTGGGATCATCAAGTGCCTGAGCAGGAGCTGACTAAAGAATTTATTGATGTCTTATTATTTTTAC
AAAAACAAAATCGTGAGATATTGATACGACAATACATAGAAAAATCCAGAAAAACTGGCTTAACAGAAGCTGAACGGCTC
AGTTTACAAAATTTATTAAAGGAAAGACATGGGCAAGCTGAAATTGAAAAATAA

Upstream 100 bases:

>100_bases
TGGGTAAAGTCATGGCTATCCTTAAGCCAAAATTACAAGGACGCGCGGACATGGCCCAAGTCAGCGCCCGGATAAAGGCA
AAATTAAGCTGAGTTCCAAT

Downstream 100 bases:

>100_bases
AAATACTGAAATACTGGTATGTTGCAGCTACCCAGTTTATAATTTAAAACTTTTTGTAATTTCCCATTGTTCCAACAGAA
GTGCCTATGACCATGAATGA

Product: DNA primase DnaG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQ
GFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVSQYYQKKLKYNGQTAIEYLRNRGLSGEIAKRYHLGYAPEGW
HNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKDQKPKYLNSPETVLFQKSRELYG
LHQILSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLP
HLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSY
KQLLIDDLARLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLLQNPEIYSIAIQQINPALLHEQEHHILLKLL
QQLKDKPNANTATLIEFWRNSSYFELIVKLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERL
SLQNLLKERHGQAEIEK

Sequences:

>Translated_577_residues
MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQ
GFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVSQYYQKKLKYNGQTAIEYLRNRGLSGEIAKRYHLGYAPEGW
HNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKDQKPKYLNSPETVLFQKSRELYG
LHQILSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLP
HLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSY
KQLLIDDLARLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLLQNPEIYSIAIQQINPALLHEQEHHILLKLL
QQLKDKPNANTATLIEFWRNSSYFELIVKLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERL
SLQNLLKERHGQAEIEK
>Mature_576_residues
SGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQG
FTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVSQYYQKKLKYNGQTAIEYLRNRGLSGEIAKRYHLGYAPEGWH
NLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKDQKPKYLNSPETVLFQKSRELYGL
HQILSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLPH
LNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSYK
QLLIDDLARLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLLQNPEIYSIAIQQINPALLHEQEHHILLKLLQ
QLKDKPNANTATLIEFWRNSSYFELIVKLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERLS
LQNLLKERHGQAEIEK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=586, Percent_Identity=43.1740614334471, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 65587; Mature: 65456

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYH
CCCCCCCHHHHHHHCHHHHHHHHHCCCCHHHCCCCCEEEECCCCCCCCCCEEEEHHHHHE
CFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVS
EECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QYYQKKLKYNGQTAIEYLRNRGLSGEIAKRYHLGYAPEGWHNLEKAFPNNQRELLSTGML
HHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCE
IKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKDQKPKYLNSPETVLFQKSRELYG
EECCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LHQILSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFC
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHEECCCCEEEEE
FDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATP
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHCH
LHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSYKQLLIDDLARLTHIESHRLT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NLITDKSESKPEVQAAISRSPLRIAIALLLQNPEIYSIAIQQINPALLHEQEHHILLKLL
HHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHCCHHHHCCCHHHHHHHHH
QQLKDKPNANTATLIEFWRNSSYFELIVKLAAWDHQVPEQELTKEFIDVLLFLQKQNREI
HHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHH
LIRQYIEKSRKTGLTEAERLSLQNLLKERHGQAEIEK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYH
CCCCCCHHHHHHHCHHHHHHHHHCCCCHHHCCCCCEEEECCCCCCCCCCEEEEHHHHHE
CFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVS
EECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QYYQKKLKYNGQTAIEYLRNRGLSGEIAKRYHLGYAPEGWHNLEKAFPNNQRELLSTGML
HHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCE
IKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKDQKPKYLNSPETVLFQKSRELYG
EECCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LHQILSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFC
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHEECCCCEEEEE
FDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATP
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHCH
LHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSYKQLLIDDLARLTHIESHRLT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NLITDKSESKPEVQAAISRSPLRIAIALLLQNPEIYSIAIQQINPALLHEQEHHILLKLL
HHHCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHCCHHHHCCCHHHHHHHHH
QQLKDKPNANTATLIEFWRNSSYFELIVKLAAWDHQVPEQELTKEFIDVLLFLQKQNREI
HHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHH
LIRQYIEKSRKTGLTEAERLSLQNLLKERHGQAEIEK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA