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The map label for this gene is kdtA [H]

Identifier: 148359880

GI number: 148359880

Start: 2756312

End: 2757571

Strand: Reverse

Name: kdtA [H]

Synonym: LPC_1808

Alternate gene names: 148359880

Gene position: 2757571-2756312 (Counterclockwise)

Preceding gene: 148359882

Following gene: 148359873

Centisome position: 77.1

GC content: 40.63

Gene sequence:

>1260_bases
ATGCGTTTTGTGTATTCCTTTTTAATGTACTTACTGACTCCTTACTTGCTTTTTCGCTTGTGGCGGAAAGGTAGAAAGTT
GCCAGCTTACAGGCAAAGGATAGGAGAGCGTTTTTGTCTGGGAATACAAGAAAATGCACCAGTTGATGTATGGCTTCATG
CGGTTTCTCTGGGAGAAGTGATCGCGGCAACACCTTTAATTGATGCAATGCTCAACAAACGTTGGTCAGTGTTAGTCACC
ACAATGACCCCTACAGGTTCTGAACGAGTAAAATCTCGATTTGGTCATAAGGTAGCGCATCAATATTTACCTTATGATTT
ACCTTGGGTTCTCAAACGATTTTTTAAAAGAACGAGACCTCGTGTCGGTATCATTATGGAAACTGAATTGTGGCCTAATT
TAATTAACCAGGCTCAAGCTTCAGGAGTGGCATTATTTCTGGCGAATGGACGCTTATCTGATCGCTCCTTGCAAGGATAT
CTAAAGCTCAAATTTTTATTTAAACCGGTTTTGAATCAATTTTCAGGTATTTTGACTCAAAGCAATGAAGATGCTGAGAG
ATTCATTGCCTTGGGTGCAAATGCCGATTTAGTCCATGTATTAGGGAATATGAAATTTGATTTACAGATTAACTCTGTTG
ATCGTAGTCAATACAGGGAATTAAAAAATCATTGGGGAGAGGATAGACTTACCGTCATTGCAGCAAGTACTCATGACGAC
GAGGAGTCTCAGATTTTATCTCAGCTACCTCGTTTGCAGGAAGCTATTCCGGGAGTGGTGTTATTAATTGCTCCGCGTCA
TCCAGAACGGTTTCAAACGGTCTATCAGTTGAGTGTTCAAGCAGGATTTAATACTGGGTGTCGTAGCAATTTGGATACGG
TTTCCAGAGAAAATGAAGTCGTTATACTCGACAGCTTGGGTGAGCTTTTGGGATTTTATCAAATTAGTGATTTTGCTTTT
GTTGGCGGCAGCCTTGTCCCGGTTGGTGGTCATAATGTATTGGAGCCAATCGCGATGAATGTGCCTGTTTTAAGCGGGAA
TCAAGTGCATAACTTTAAATCAATTTGCAGAGAGCTTAAAGAGGCTCAGGCAATTTTGCTAGTGAATCATGCCAATGAGT
TGGTTGATGCAATTATCCAGTTATATCAGGATAAGGAATCTCAAAACACTATGGTTGCAAACGCCAGTTCAGTCCTTGAA
AGTAATAAGGGCTCTGTAGTGAGGTATTTACAGAAAATTGAGTCAGTTTTGGGATATTAG

Upstream 100 bases:

>100_bases
TATAGATTGAGGACAAGATAGTCTCTAATTGAATTGTAACATATCGCACAAGGAACTGTTTTAGGTATATACTTTACCTT
TTTGTGTTTGGTGCCTGTTT

Downstream 100 bases:

>100_bases
CTTGTTATAAAATTCTCTCCATATTGATTTCTTGTATCATACCAAAAAATTCATCAAAACATTTTTGAACTTGTTCAAGG
TGGAGTATCCATGGACAATG

Product: 3-deoxy-D-manno-oct-2-ulosonic acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEVIAATPLIDAMLNKRWSVLVT
TMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTRPRVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGY
LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQYRELKNHWGEDRLTVIAASTHDD
EESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTVSRENEVVILDSLGELLGFYQISDFAF
VGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIQLYQDKESQNTMVANASSVLE
SNKGSVVRYLQKIESVLGY

Sequences:

>Translated_419_residues
MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEVIAATPLIDAMLNKRWSVLVT
TMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTRPRVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGY
LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQYRELKNHWGEDRLTVIAASTHDD
EESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTVSRENEVVILDSLGELLGFYQISDFAF
VGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIQLYQDKESQNTMVANASSVLE
SNKGSVVRYLQKIESVLGY
>Mature_419_residues
MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEVIAATPLIDAMLNKRWSVLVT
TMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTRPRVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGY
LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQYRELKNHWGEDRLTVIAASTHDD
EESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTVSRENEVVILDSLGELLGFYQISDFAF
VGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIQLYQDKESQNTMVANASSVLE
SNKGSVVRYLQKIESVLGY

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=419, Percent_Identity=42.2434367541766, Blast_Score=334, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47338; Mature: 47338

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHH
IAATPLIDAMLNKRWSVLVTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTRP
HHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGYLKLKFLFKPVLNQFSGILTQ
CEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQYRELKNHWGEDRLTVIAASTHDD
CCCCHHHHEEECCCCCHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCC
EESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTVSRENEV
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCE
VILDSLGELLGFYQISDFAFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELK
EEEECHHHHHHHHHHCCHHEECCCEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
EAQAILLVNHANELVDAIIQLYQDKESQNTMVANASSVLESNKGSVVRYLQKIESVLGY
HCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQENAPVDVWLHAVSLGEV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHH
IAATPLIDAMLNKRWSVLVTTMTPTGSERVKSRFGHKVAHQYLPYDLPWVLKRFFKRTRP
HHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
RVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGYLKLKFLFKPVLNQFSGILTQ
CEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQYRELKNHWGEDRLTVIAASTHDD
CCCCHHHHEEECCCCCHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCC
EESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQLSVQAGFNTGCRSNLDTVSRENEV
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCE
VILDSLGELLGFYQISDFAFVGGSLVPVGGHNVLEPIAMNVPVLSGNQVHNFKSICRELK
EEEECHHHHHHHHHHCCHHEECCCEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
EAQAILLVNHANELVDAIIQLYQDKESQNTMVANASSVLESNKGSVVRYLQKIESVLGY
HCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]