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The map label for this gene is prfA [H]

Identifier: 148359876

GI number: 148359876

Start: 2752885

End: 2753973

Strand: Direct

Name: prfA [H]

Synonym: LPC_1804

Alternate gene names: 148359876

Gene position: 2752885-2753973 (Clockwise)

Preceding gene: 148359875

Following gene: 148359877

Centisome position: 76.97

GC content: 42.06

Gene sequence:

>1089_bases
ATGAAGAAATCTCTTGAGTTAAAATTACAGCAAATGTTGGAGCGATACGAGGAAGTAGGCCGCTTATTGTCCGAAGCATC
CATTATTGCCGATCAAAATCAATTTAAGTCTTTATCAAAAGAATATGCCCAACTTGAACCAGTTTCCCAATGTTATGAGT
CGTATTTAGAGGCAAAAAATAATTTGGATTCTTTAAACGAATTGTTGGAAAGTGATGATAAAGATTTAGCGACTATGGCT
GAAGAAGAGATAGACATCGTCAAGAAACAAATTGAGGAATTGGATGAACAATTACAATGGCATCTCATTCCTAAAGATCC
AGATGATGAACGCAATATTTATCTTGAAGTCAGAGCCGGCACAGGTGGAGACGAAGCGGCTATATTTGCCGGGGATCTCT
TTCGAATGTACAGCCGTTATGCAGAAAGCCAGGGTTGGCAAATTGAATTAATTAGCGCCAGCCATGGTGAACATGGAGGC
TACAAGGAAATAATTGCCAAAATCAGTGGGCAAGCGGTTTATTCACAACTCAAATTTGAATCAGGAGCTCACAGAGTACA
GAGAGTACCTGAAACTGAATCGCAAGGTCGTGTCCATACTTCCGCATGCACGGTTGCTATCATGCCTGAGGTGGATGAAA
TTAATGACATTCAGATAAATCCCGATGACTTACGCATCGATACTTACAGATCTTCTGGAGCCGGTGGACAGCATGTTAAC
AAAACAGATTCTGCCATTCGTATCACACACATCCCTACAGGAGTGGTTGTAGAGTGCCAGGATGAGCGTTCACAGCACAA
AAACCGTGCCAAAGCAATGTCCTTGTTAAAAACAAGGTTATTAGATGCCGAAGTAAGCAAACAAAAACAAGAACAAGCTC
AAACACGTAAGTCACTGGTTGGTACAGGTGACCGCTCCGAGCGCATTAGAACTTATAACTTTCCTCAAGGTCGGCTCACT
GATCATCGTATCAATCTGACTATCTACCAGCTTAGTGACATCATGGAAGGGAATTTATCCCTGGTCATTGACCCGTTAAA
ACGCGAATACCATGCTGAACTGCTAGCCGATTTAGGTCGACATGATTGA

Upstream 100 bases:

>100_bases
AACCCCACCATAGGTTTAAGACAAATAGCATGGGATAACAGAGAAGACTTACTTGACCTCGCACGATATCTCTTTGATAC
AACAGCCAATCAATCATTAT

Downstream 100 bases:

>100_bases
TATTCGTTCATTACTGGAACAAGCTCTGCAACAATTCAAAGTCAAGAACCAGGAAACCAAGTTAGAGGCAGAACTTTTAC
TCTGCCATGTGTTAAACAAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMA
EEEIDIVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLT
DHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGRHD

Sequences:

>Translated_362_residues
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMA
EEEIDIVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLT
DHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGRHD
>Mature_362_residues
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMA
EEEIDIVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLT
DHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGRHD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=285, Percent_Identity=49.1228070175439, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI34577120, Length=343, Percent_Identity=41.1078717201166, Blast_Score=261, Evalue=9e-70,
Organism=Homo sapiens, GI166795305, Length=197, Percent_Identity=49.746192893401, Blast_Score=198, Evalue=7e-51,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=62.5698324022346, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=39.2215568862275, Blast_Score=231, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=271, Percent_Identity=33.9483394833948, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=306, Percent_Identity=42.156862745098, Blast_Score=242, Evalue=7e-65,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=39.0909090909091, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41116; Mature: 41116

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NLDSLNELLESDDKDLATMAEEEIDIVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEEEC
TGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGGYKEIIAKISGQAVYSQLKFE
CCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHH
SGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
HHHHHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCC
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGR
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHCC
HD
CC
>Mature Secondary Structure
MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQLEPVSQCYESYLEAKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NLDSLNELLESDDKDLATMAEEEIDIVKKQIEELDEQLQWHLIPKDPDDERNIYLEVRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCEEEEEEEC
TGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGGYKEIIAKISGQAVYSQLKFE
CCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHH
SGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQINPDDLRIDTYRSSGAGGQHVN
HHHHHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCEECCCCEEEEEECCCCCCCCCCC
KTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLLKTRLLDAEVSKQKQEQAQTRKSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDIMEGNLSLVIDPLKREYHAELLADLGR
CCCCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHCC
HD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA