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The map label for this gene is fadE25 [H]

Identifier: 148359768

GI number: 148359768

Start: 2631914

End: 2633605

Strand: Reverse

Name: fadE25 [H]

Synonym: LPC_1693

Alternate gene names: 148359768

Gene position: 2633605-2631914 (Counterclockwise)

Preceding gene: 148359769

Following gene: 148359767

Centisome position: 73.64

GC content: 40.37

Gene sequence:

>1692_bases
ATGACTTCTTATGAAAATTTGATACAACTGTCTGAATTTTTTGAGTCATGTCTGGGTAATCCTTTGGATGACAATTCTCC
TGTTAATTTTCAACAAATTTTGCTTGCTGATGAGCAAGAAATATTAGCTTCGTCAGCAATAGAATTTATTAAACAATGGG
GGTATTTGGACTATACCATACCTCATTATCTAGGGGGTAAGCTGAACTCCCTGGATGAGCTGTATACTTTAACCAGGTTG
TTGGCCAGGCGTGACATCACGATGGCTATTATGTTTGGATTGGCTTTCTTTGCTGCATTGCCAATATGGGTGGCTGGAAA
CTCAAAGCAAAAGAAACATTTGAGTGAGAGCCTTCGCCAGGGAAAGATAGGTGCTTTGGCTTTGACCGAAGAAGAGCATG
GAACCGATTTGACTTCCAATAAGATGAATGCCAAGCCAACTGAAGATGGCTGGGAAATTTCGGGGCGAAAATGGTGTGTT
AATTTTGCAACCTTGAGCGAGTACGCTGTGGTGTTGTGTCGGACTAATGAGAAGGGCGGGTTATTAGGTTTTTCTTTGTT
TTATGTCGATAAAAAACTAGCTGAAAAGGGTATTTCTCCTACACCTAAGCTTCCGACTCATGGGGTCCGAGGGTTAGATA
TCAGTGGTTTTTCTTTTGATAAAGTGAAACTGTCTAAAGACGCATTAATAGGGAAGGAACAACGTGGTTTGGAAATAACC
TATAAGATATTCCAGGTTTCGCGAACCTTATGTGCCTGCTTGGCGATCGGGGGTGCTGATACTGCTTTGAGGCTGGCATT
ATCATACAGCTTGCAAAGACAACTGTATGGCAAATCAGTGTTTGAAATACCCGCAGTAAAACAACGGCTGGGCGAATTGT
TTACTCTTTTATTAATCGCGGATTGCACTGCCCAGGTTATGGTCCGCGCCTGTACGTTAATACCGGAAAAAATGAGTCTT
TGGTCAGCAATTATCAAATTTCTTATTCCGCATATTGGAGAAGATATAGCAGAACAGTGTGCCATTATTCTAGGTGCCAG
GGCTTATCTGAGAACCACGCAATGGGCTATTTTTCAGAAAATAAGACGTGATATTCAAGTAGTTGGTTTGTTTGATGGAA
GTACTCAAGTTAATTTATCCCTTATTGCGGGTAATTTGTTGCCCCAGGCAGGCATGCGGGGTAAAAATCGGGCAGAGCAT
TCCGAAAAAATTGAAAAAATTTTTAATATTCGTTTGTCTTGTCCTCCTTTTAAGGAGGATAGACTCGGTTTGTTTACTCA
TGAAGAAGATGATGTATTGGCTGGTATCCTGTCGTTAAACTCAATTCCAATCAATCCGTTGATCACTACAATACGTCATG
AGATTGAAAAATTGGATCAAGAAGTCATCCGTTTACATGATCAGAAATTATTTGACCCACGCAGTCTAGCCGTTTTTCGT
TTGGCAGAAAAATATTGCTGGATTTTTGCTGCCAGTTGTTGTTTGCAGTTCTGGCACTATAATCAGGAGATACTTTCTGA
TGATTTGCAAAATACAGATTGGCTGGATTTAGCGATGCAGTTAATTCTTAAGCGATTGAATTCAGGTTCGATGATTGATA
GCGTGTTGCAAGAAGCTATGAGTGAGCGTTTGTATTCTTTTTATCAGGAAAAACAGCTGTTTTCTATTATGCCAATACAT
ATTGCGGGGTAA

Upstream 100 bases:

>100_bases
CAGCACAAATTCGCAGTCGCCTTGCGTCTCATTTGGAATTTTTATATCGAAGAATGGAAATCATGCCTCCGGCAAAACAC
TCTATTGTATAAGGAAAATA

Downstream 100 bases:

>100_bases
TGAGGGATGAATTGGAAGAGATTTTTAATTGCTGCCATGACAAAGAAAAAATACCAGGTTTTTTTGAATGATACCAAGCA
TCCTAAAGAAGCGAGAGAAC

Product: isovaleryl CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTSYENLIQLSEFFESCLGNPLDDNSPVNFQQILLADEQEILASSAIEFIKQWGYLDYTIPHYLGGKLNSLDELYTLTRL
LARRDITMAIMFGLAFFAALPIWVAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCV
NFATLSEYAVVLCRTNEKGGLLGFSLFYVDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKLSKDALIGKEQRGLEIT
YKIFQVSRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTLIPEKMSL
WSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKNRAEH
SEKIEKIFNIRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDQKLFDPRSLAVFR
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIH
IAG

Sequences:

>Translated_563_residues
MTSYENLIQLSEFFESCLGNPLDDNSPVNFQQILLADEQEILASSAIEFIKQWGYLDYTIPHYLGGKLNSLDELYTLTRL
LARRDITMAIMFGLAFFAALPIWVAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCV
NFATLSEYAVVLCRTNEKGGLLGFSLFYVDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKLSKDALIGKEQRGLEIT
YKIFQVSRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTLIPEKMSL
WSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKNRAEH
SEKIEKIFNIRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDQKLFDPRSLAVFR
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIH
IAG
>Mature_562_residues
TSYENLIQLSEFFESCLGNPLDDNSPVNFQQILLADEQEILASSAIEFIKQWGYLDYTIPHYLGGKLNSLDELYTLTRLL
ARRDITMAIMFGLAFFAALPIWVAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCVN
FATLSEYAVVLCRTNEKGGLLGFSLFYVDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKLSKDALIGKEQRGLEITY
KIFQVSRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTLIPEKMSLW
SAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKNRAEHS
EKIEKIFNIRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDQKLFDPRSLAVFRL
AEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIHI
AG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501857, Length=285, Percent_Identity=28.7719298245614, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI4557233, Length=360, Percent_Identity=24.1666666666667, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4557231, Length=290, Percent_Identity=25.1724137931034, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI187960098, Length=290, Percent_Identity=25.1724137931034, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI76496475, Length=380, Percent_Identity=18.9473684210526, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4557235, Length=380, Percent_Identity=18.9473684210526, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI226958412, Length=298, Percent_Identity=24.1610738255034, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI226958414, Length=276, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508101, Length=352, Percent_Identity=21.0227272727273, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538396, Length=336, Percent_Identity=22.3214285714286, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985184, Length=397, Percent_Identity=20.1511335012594, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=360, Percent_Identity=23.3333333333333, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534899, Length=367, Percent_Identity=23.1607629427793, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17569725, Length=361, Percent_Identity=21.8836565096953, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982178, Length=223, Percent_Identity=23.7668161434978, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506239, Length=336, Percent_Identity=20.2380952380952, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71990804, Length=243, Percent_Identity=21.3991769547325, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356377, Length=363, Percent_Identity=25.3443526170799, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24660351, Length=354, Percent_Identity=25.1412429378531, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24646207, Length=360, Percent_Identity=22.5, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24666513, Length=282, Percent_Identity=21.9858156028369, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21355753, Length=284, Percent_Identity=22.5352112676056, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920834, Length=366, Percent_Identity=24.0437158469945, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 63503; Mature: 63372

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYENLIQLSEFFESCLGNPLDDNSPVNFQQILLADEQEILASSAIEFIKQWGYLDYTI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEC
PHYLGGKLNSLDELYTLTRLLARRDITMAIMFGLAFFAALPIWVAGNSKQKKHLSESLRQ
HHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHC
GKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCVNFATLSEYAVVLCRTNEKGG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEHHHHCCEEEEEEEECCCCC
LLGFSLFYVDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKLSKDALIGKEQRGLEIT
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCCCCCCCHHH
YKIFQVSRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DCTAQVMVRACTLIPEKMSLWSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKNRAEHSEKIEKIFNIRLSCPPFKED
HHHCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
RLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDQKLFDPRSLAVFR
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SERLYSFYQEKQLFSIMPIHIAG
HHHHHHHHHHHHHHEECCEEECC
>Mature Secondary Structure 
TSYENLIQLSEFFESCLGNPLDDNSPVNFQQILLADEQEILASSAIEFIKQWGYLDYTI
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEC
PHYLGGKLNSLDELYTLTRLLARRDITMAIMFGLAFFAALPIWVAGNSKQKKHLSESLRQ
HHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHC
GKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCVNFATLSEYAVVLCRTNEKGG
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEHHHHCCEEEEEEEECCCCC
LLGFSLFYVDKKLAEKGISPTPKLPTHGVRGLDISGFSFDKVKLSKDALIGKEQRGLEIT
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHCCCCCCCCHHH
YKIFQVSRTLCACLAIGGADTALRLALSYSLQRQLYGKSVFEIPAVKQRLGELFTLLLIA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DCTAQVMVRACTLIPEKMSLWSAIIKFLIPHIGEDIAEQCAIILGARAYLRTTQWAIFQK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRRDIQVVGLFDGSTQVNLSLIAGNLLPQAGMRGKNRAEHSEKIEKIFNIRLSCPPFKED
HHHCEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
RLGLFTHEEDDVLAGILSLNSIPINPLITTIRHEIEKLDQEVIRLHDQKLFDPRSLAVFR
CCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAEKYCWIFAASCCLQFWHYNQEILSDDLQNTDWLDLAMQLILKRLNSGSMIDSVLQEAM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SERLYSFYQEKQLFSIMPIHIAG
HHHHHHHHHHHHHHEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]