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The map label for this gene is pilD [H]

Identifier: 148359057

GI number: 148359057

Start: 1753379

End: 1754242

Strand: Direct

Name: pilD [H]

Synonym: LPC_0943

Alternate gene names: 148359057

Gene position: 1753379-1754242 (Clockwise)

Preceding gene: 148359056

Following gene: 148359062

Centisome position: 49.03

GC content: 34.95

Gene sequence:

>864_bases
ATGATAAATGCACTAATAATCAATTATCCATGGTTTATGTATTTAGTCGTTGGACTCTTTTCTCTAGCTGTTGGAAGTTT
GCTTAATGTGATAATCTATCGCTTACCAATTATTTTGCAGGAAGAGTGGAAAGAGCAATGCTGTGAGCTATTCCAGCTTG
AACAGCGAAAAGAAAAAATAAAACTTAATTTATTTTTACCACGTTCTTTTTGCCCTCATTGTAAAGCGATGGTTAAAGCG
TGGCAGAATATTCCTCTGTTAAGCTATATTTTGTTAAGAGGACGCTGTTACCAATGCGATAGTCCAATTTCAATTCGCTA
CCCTTTTGTTGAGACTCTGACCACAGTTTTATCCTTATACGCCAGTTGGCATTTTGGGTTTACAATACAACTATTATTTG
CTTTATTAGCTATCTGGATATTAATATCACTTGTTTTTATTGATCTTGATCATCAATTACTCCCTGATAGCCTCACTTTA
GGGCTCTTATGGATTGGCCTCATTGCTAATACCCAGAATATATTTGTTAGTCTTGACGTTGCTGTATTAAGCTGCGCTGG
CGCCTATTTAGCCTTATGGTTATTCATTAATCTGTTTTATCTGATGACAGGTAAAGTAGGTATGGGACATGGGGATTTCA
AATTATTTGCAGCATTTGGTGCATGGCTTGGATGGATGTATTTACCATTAATTCTACTAATATCTTCAATAACCGGGGCT
ATCATTGGTTTGATTTATTTAAAGATAAATGGTAAATCAAGAGATACTGCAATACCATTTGGCCCTTTTCTTTGCATTTC
TGGATTAATAGCTATGTTTTGGGGAGATTTAATTATCAATTGGTATATAGGTTATTGGATGTAA

Upstream 100 bases:

>100_bases
GGGATTGGTGGTGGCTATGTATTTACCTATTTTCAAACTGGGTTCTGCTGTTTAAACAGATCCTATTTAATTTAATCATA
CAATATAGGCAAACGTGTCA

Downstream 100 bases:

>100_bases
AATGTTGATTAGCTTAGCGCAACCCAACAATAAATAGAGACCACACGCCTAAAATGACATGAGAAACGGATACTCCCAAA
AGAGATTTCTGTCTGTGAAA

Product: type 4 (IV) prepilin-like protein leader peptide processing enzyme PilD

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM

Sequences:

>Translated_287_residues
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM
>Mature_287_residues
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKA
WQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
GLLWIGLIANTQNIFVSLDVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=272, Percent_Identity=36.7647058823529, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=43.1818181818182, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32801; Mature: 32801

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKI
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLY
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHH
ASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNIFVSLDV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCEEEEHHH
AVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM
HHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWKEQCCELFQLEQRKEKI
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSPISIRYPFVETLTTVLSLY
EEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHH
ASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNIFVSLDV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHCCCCEEEEHHH
AVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAAFGAWLGWMYLPLILLISSITGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGDLIINWYIGYWM
HHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9529113 [H]