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The map label for this gene is rumA [H]

Identifier: 148358986

GI number: 148358986

Start: 1670190

End: 1671524

Strand: Direct

Name: rumA [H]

Synonym: LPC_0871

Alternate gene names: 148358986

Gene position: 1670190-1671524 (Clockwise)

Preceding gene: 148358983

Following gene: 148358987

Centisome position: 46.7

GC content: 38.13

Gene sequence:

>1335_bases
ATGAGAAAAGTTAAGCCAAAATTGAATTTAACCTCACAAACCGCTCGGATCGTAAATTTAAGCCATGATGGAAGGGGGAT
AGCACGAGTTAATGGCAAAGCAACTTTTATTCAGGGAGCATTACCTGGTGAGGTGGTTGAATTTCAGTATACACGGATAA
AAAAGGATTTTGATGAAGGTAAATTATTATCAATTGTTGAGCCCTCTACTCTCAGGGTTGAACCAAAATGCCCTCATTAT
CAAATGTGTGGGGGCTGTTCTCTGCAACATATGAGTGCCGAGGAACAAATTCGTTTCAAACAATCCCATCTTTTGGATTT
ACTATCCCGGTATGGCCATACTGAACCTCAATCAGTGTTGTCGCCTTTAACAAGCCATCATTGGAATTATAGAAATAAAG
CGCGCTTAAGTACTCGCTTTGTAGAAAAGAAACAAAGCACCATGGTTGGATTTAGAGAGAGGAATAATCCAAGGTTCATT
ACAGAAATAAATCAATGTCCTATTCTTAATTCAAAAATAGATACTGATATTGTCCATTTGAGAAAATTAATCGATACGAT
GGAAGATAAGCACTGTATCGCACAAATTGAAGTGGCTGCAGGTGATAATGAAGTGGCGCTGATTTTCCGCAATCTATCTC
CATTAACAGAGCAAGATGAGTTGAAAATCAGGGAATTTGCGCAACAATTCCAATACAAGGTATTCCTGCAGCCAGGTGGA
CTTGATTCGGTTTTTTGTTTTTATCCTTCAGATGCTCATGCTTATCTGAGTTATGAGCTGCCTGATTATCAAATAACTTT
TCAATTTCATCCCAACGATTTTACTCAGGTAAACGCCGAGTTAAACAGAAAGATGGTAACGCAGGCAATTCAGTTAATGG
AATTAAAAAATTCTGATATCGTGCTAGACTTATTTTGTGGACTGGGTAATTTTTCGCTTCCCATGGCTAAACATTGCTCT
CGAGTTATCGGTGTCGAAGGCAATAAGAACATGGTTGAAAGAGCCTATATGAATGCCAAGTCTAATCATATTACCAATGT
GGATTTTTATGCCGCTAATCTTGATGATGTGATGGAAGTTAGGAATCTGGTTAATACGTCTTTTAGCAAGGTTTTAATCG
ATCCTCCCCGGTCTGGTGCGCTTGAAATTGTAAAACAAATTGATTCAATTGATCCCGAGCGTATAGTATATGTATCTTGT
AACCCGATAACATTAGCTCGAGATACAGATATATTGGTTAATCAAAAAGGTTACGTTTTGATAACAGCTGGGGTAATGGA
TATGTTTCCTCATACAGCGCATGTCGAGTCTATTGCCTTGTTTCAAAAAGGATAA

Upstream 100 bases:

>100_bases
TGGCCCTTTATTCGGGCATTTTTTGTTTTTTATATCCATACAACATTCTAAAAATGTAGCATGAGAATTTTGTAATTGAT
AATTATTTGAGAATCATTTC

Downstream 100 bases:

>100_bases
TATGTATGGTAAGAGTAAAAGATACGACTCCGTTGATGCCAGATGGCAGTATCGACGTAGAGATGTGGTTACATCATCTT
GGTTCAAAAGGATACTTGGA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEVVEFQYTRIKKDFDEGKLLSIVEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSVLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQDELKIREFAQQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG

Sequences:

>Translated_444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEVVEFQYTRIKKDFDEGKLLSIVEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSVLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQDELKIREFAQQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG
>Mature_444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEVVEFQYTRIKKDFDEGKLLSIVEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSVLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQDELKIREFAQQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=264, Percent_Identity=30.6818181818182, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI51173878, Length=264, Percent_Identity=30.6818181818182, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI269784680, Length=257, Percent_Identity=25.2918287937743, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI269784676, Length=257, Percent_Identity=25.2918287937743, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI20149688, Length=257, Percent_Identity=25.2918287937743, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI269784678, Length=257, Percent_Identity=25.2918287937743, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789148, Length=433, Percent_Identity=36.0277136258661, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790403, Length=177, Percent_Identity=32.2033898305085, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787083, Length=180, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=34.3434343434343, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50498; Mature: 50498

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEVVEFQYTRIKKDFDEG
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCCCCEEEEEHHHHHCCCCCC
KLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSVL
CEEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFITEINQCPILNSKIDTDIVHL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
RKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQDELKIREFAQQFQYKVFLQPGG
HHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCC
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDI
CCCEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCCE
VLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEV
EEEEEECCCCCCCCHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
RNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSCNPITLARDTDILVNQKGYVL
HHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCEEE
ITAGVMDMFPHTAHVESIALFQKG
EEECHHHHCCCHHHHHEEEEEECC
>Mature Secondary Structure
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEVVEFQYTRIKKDFDEG
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCCCCEEEEEHHHHHCCCCCC
KLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSVL
CEEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFITEINQCPILNSKIDTDIVHL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
RKLIDTMEDKHCIAQIEVAAGDNEVALIFRNLSPLTEQDELKIREFAQQFQYKVFLQPGG
HHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCC
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDI
CCCEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCCE
VLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEV
EEEEEECCCCCCCCHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
RNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSCNPITLARDTDILVNQKGYVL
HHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCEEE
ITAGVMDMFPHTAHVESIALFQKG
EEECHHHHCCCHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA